Development of a Ladder-Shape Melting Temperature Isothermal Amplification (LMTIA) Assay for the Identification of Cassava Component in Sweet Potato Starch Noodles
Abstract
:1. Introduction
2. Results
2.1. Optimization of the LMTIA Reaction Temperature
2.2. Specificity Determination of the LMTIA Assay
2.3. Sensitivity Determination of the LMTIA Assay
2.4. Suitability Determination of the LMTIA Assay
2.5. Cassava Component Detection in Sweet Potato Starch Noodles
3. Discussion
4. Materials and Methods
4.1. Selection of the Target Sequences for LMTIA Primer Design
4.2. Primer Design for LMTIA Assay
4.3. Genomic DNA Extraction
4.4. Optimization of the LMTIA Reaction Temperature
4.5. Specificity Determination of the LMTIA Assay
4.6. Sensitivity Determination of the LMTIA Assay
4.7. Suitability Determination of the LMTIA Assay
4.8. Cassava Component Detection in Sweet Potato Starch Noodles
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
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Primer | Sequence (5′–3′) |
---|---|
P (P2–tttt–P1) | TGTCCGAGGGTCTTTTTTCACGGCTATCGGTGGT |
D (D2–tttt–D1) | GCCGTGGGCGAACTTTTGGTCCCGTTCGGCAGAC |
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Zhang, Y.; Wang, Y.; Ouyang, X.; Wang, D.; Xiao, F.; Sun, J. Development of a Ladder-Shape Melting Temperature Isothermal Amplification (LMTIA) Assay for the Identification of Cassava Component in Sweet Potato Starch Noodles. Molecules 2022, 27, 3414. https://doi.org/10.3390/molecules27113414
Zhang Y, Wang Y, Ouyang X, Wang D, Xiao F, Sun J. Development of a Ladder-Shape Melting Temperature Isothermal Amplification (LMTIA) Assay for the Identification of Cassava Component in Sweet Potato Starch Noodles. Molecules. 2022; 27(11):3414. https://doi.org/10.3390/molecules27113414
Chicago/Turabian StyleZhang, Yongqing, Yongzhen Wang, Xingmei Ouyang, Deguo Wang, Fugang Xiao, and Juntao Sun. 2022. "Development of a Ladder-Shape Melting Temperature Isothermal Amplification (LMTIA) Assay for the Identification of Cassava Component in Sweet Potato Starch Noodles" Molecules 27, no. 11: 3414. https://doi.org/10.3390/molecules27113414