Extraction of Pectin from Satsuma Mandarin Peel: A Comparison of High Hydrostatic Pressure and Conventional Extractions in Different Acids
Round 1
Reviewer 1 Report
I have read the manuscript “Effects of high pressure and heating on the extraction of Satsuma mandarin peel pectin” which described a theoretical basis for high-value comprehensive utilization of by-products of Satsuma mandarin, and expand the application of HHP in the efficient extraction of pectin. I have examined the submitted paper very carefully and I recommend its publication after Authors consider changing the manuscript title under the next considerations:
In the title, the Authors claim that pressure and heating will be analyzed in this work. However, there is only one method analyzed (high hydrostatic pressure, HHP) against conventional extraction. What I can see is a good analysis using acidic conditions in both methods. So, I consider this the main objective in this work.
Author Response
I have read the manuscript “Effects of high pressure and heating on the extraction of Satsuma mandarin peel pectin” which described a theoretical basis for high-value comprehensive utilization of by-products of Satsuma mandarin, and expand the application of HHP in the efficient extraction of pectin. I have examined the submitted paper very carefully and I recommend its publication after Authors consider changing the manuscript title under the next considerations:
In the title, the Authors claim that pressure and heating will be analyzed in this work. However, there is only one method analyzed (high hydrostatic pressure, HHP) against conventional extraction. What I can see is a good analysis using acidic conditions in both methods. So, I consider this the main objective in this work.
Dear reviewer:
Thank you so much for your valuable comments and suggestions. We have revised the title accordingly. Please see Line 1-3. All the modifications in the revised manuscript were highlighted with red font.
Reviewer 2 Report
The manuscript "Effects of high pressure and heating on the extraction of Satsuma mandarin peel pectin" is devoted to high hydrostatic pressure assisted extraction of pectin from Satsuma mandarin peel. Citric or hydrochloric acids are used as extragents. Pectin was determined by the colorimetric method and was characterized by FTIR and NMR spectroscopy. Also, rheological and emulsion properties were studied. It has been obtained that hydrostatic pressure assisted extraction with citric acid solution more efficient in compare with conventional extraction and high pressure assisted extraction with hydrochloric acid. The results of this work may be useful for improving of plant material processing.
The manuscript is well structured, some needed information is presented in Supplementary materials, and methods of isolation are described in detail. I think the manuscript may be published in the Molecules journal after minor revision after taking into account some of the remarks described below
1. Line 130: “FTIR spectroscopy measurements were determined…” – I think, it would be better to say “… were performed…”
2. Figure 3: It would be better if the FTIR lines were presented in the stack-line graph with one Y-axis, but no with separate Y-axis. Peaks should be signed.
3. Section 3.1. Extraction yield of pectin: I think, Tukey HSD test should be performed for the extraction yield of pectins.
Author Response
The manuscript "Effects of high pressure and heating on the extraction of Satsuma mandarin peel pectin" is devoted to high hydrostatic pressure assisted extraction of pectin from Satsuma mandarin peel. Citric or hydrochloric acids are used as extragents. Pectin was determined by the colorimetric method and was characterized by FTIR and NMR spectroscopy. Also, rheological and emulsion properties were studied. It has been obtained that hydrostatic pressure assisted extraction with citric acid solution more efficient in compare with conventional extraction and high pressure assisted extraction with hydrochloric acid. The results of this work may be useful for improving of plant material processing.
The manuscript is well structured, some needed information is presented in Supplementary materials, and methods of isolation are described in detail. I think the manuscript may be published in the Molecules journal after minor revision after taking into account some of the remarks described below
Dear reviewer:
Thank you so much for your valuable comments and suggestions. We have revised the manuscript accordingly, and the detailed corrections are listed below point by point. English expression of the paper has been revised thoroughly by the commercial language editing service. All the modifications in the revised manuscript were highlighted with red font.
- Line 130: “FTIR spectroscopy measurements were determined…” – I think, it would be better to say “… were performed…”
A: Thank you so much for your valuable comments. It has been revised accordingly in the manuscript. Please see Line 133-134.
- Figure 3: It would be better if the FTIR lines were presented in the stack-line graph with one Y-axis, but no with separate Y-axis. Peaks should be signed.
A: Thank you so much for your valuable comments. It has been revised accordingly in the manuscript. Please see Figure 3.
- Section 3.1. Extraction yield of pectin: I think, Tukey HSD test should be performed for the extraction yield of pectins.
A: Thank you so much for your valuable comments. It has been revised accordingly in the manuscript. Please see Figure 1.
