Inclusion Complex between Local Anesthetic/2-hydroxypropyl-β-cyclodextrin in Stealth Liposome

Round 1

Reviewer 1 Report

In the present work, Souza and his/her co-workers investigated on developing a formulation for local anesthetic 85 butamben (BTB) and ropivacaine (RVC) in a binary system through its complexation in 86 2-hydroxypropyl-β-cyclodextrin (HP-βCD), followed by encapsulation in stealth lipo-87 somes. I found this paper valuable for the journal readers after some minor revisions:

1- Don't use bulk references.

2- The quality of Fig. 2 should be improved.

3- There are some grammatical mistakes. The manuscript should be revised in the term of the English language by a native person.

4- The encapsulation mechanism of drugs inside the liposome vesicle should be described in detail in the introduction section.

5- I think it's better to show the x-axis in figure 1 in the log scale.

6- The x and y font label size in figure 1 should be increased.

7- The main outcome of this research should be validated by the previous studies.

8- The Materials and Methods section should be moved before the results section.

9- results section should be renamed to "Results and discussion".

10- All the equations need references.

11- The conclusion section is presented poorly. It should be improved.

12- The following references could help you to make a good story in the introduction section for the encapsulation process:

https://doi.org/10.3390/molecules26164920

https://doi.org/10.1038/s41598-021-98222-2

doi: 10.2147/IJN.S298699

Author Response

 Thank you for your valuable suggestion.

Author Response File: Author Response.pdf

Reviewer 2 Report

In this manuscript, the authors present very important information about the effective delivery of local anesthetics (ropivacaine and butamben) via cd-liposomal capsulated in ternary systems for prolonged anesthesia with reduced cytotoxicity. The manuscript is worth publishing in Molecules if the authors perform more experimental work. I have the following comments:

·         Authors claim sustained release of local anesthetic drugs from cyclodextrin encapsulated but however authors failed to perform drug release studies.

·         Authors have not performed any experiments to demonstrate the stability of cyclodextrin drug encapsulated liposomes. I suggest that others provide a brief description of the stability aspects of the drug encapsulated liposomes. Provide supportive references about similarly performed works.

·         Provide the slope and correlation coefficient (r2) value in Figure 1. Please also report the standard deviations of the plots.

Author Response

Thank you for your valuable suggestion.

Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

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