Characterization and Antioxidant Activity of Mannans from Saccharomyces cerevisiae with Different Molecular Weight
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Scanning Electron Microscope (SEM)
2.3. Molecular Weight Characterization of Mannans
2.4. FTIR
2.5. Measurements of Free Radical Scavenging Activity of Mannans
2.5.1. Measurements of Reducing Power of Mannans
2.5.2. Measure of DPPH Radicals Scavenging Activity of Mannans
2.5.3. Measure of Hydroxyl Radicals Scavenging Activity of Mannans
2.6. Cytotoxicity of Mannans in Caco-2 Cells
2.7. Determination of Hemolytic Activity
2.8. Statistical Analysis
3. Results and Discussions
3.1. Morphology of Mannans
3.2. Molecular Weight of Native Mannans
3.3. Molecular Structure of Mannans
3.4. Antioxidant of Mannans In Vitro
3.4.1. Effect on Ferric Reducing Power
3.4.2. Effect of Scavenging on DPPH Radicals
3.4.3. Effect of Scavenging on Hydroxyl Radicals
3.5. Cytotoxicity of Mannans on Caco-2 Cells
3.6. Hemolytic Activity of Mannans
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Mannan Samples | Molecular Weight (Da) | Filtration Volume (mL) | Partition Coefficient (Kav) |
---|---|---|---|
native YM50 | 172,901 | 112.5 | 0.0917 |
native YM70 | 87,096 | 144 | 0.209 |
native YM90 | 54,050 | 166.5 | 0.294 |
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Zhao, Y.; Wang, J.; Fu, Q.; Zhang, H.; Liang, J.; Xue, W.; Zhao, G.; Oda, H. Characterization and Antioxidant Activity of Mannans from Saccharomyces cerevisiae with Different Molecular Weight. Molecules 2022, 27, 4439. https://doi.org/10.3390/molecules27144439
Zhao Y, Wang J, Fu Q, Zhang H, Liang J, Xue W, Zhao G, Oda H. Characterization and Antioxidant Activity of Mannans from Saccharomyces cerevisiae with Different Molecular Weight. Molecules. 2022; 27(14):4439. https://doi.org/10.3390/molecules27144439
Chicago/Turabian StyleZhao, Yingyuan, Jiaqi Wang, Qianzhen Fu, Huiru Zhang, Jin Liang, Wenjie Xue, Guoqun Zhao, and Hiroaki Oda. 2022. "Characterization and Antioxidant Activity of Mannans from Saccharomyces cerevisiae with Different Molecular Weight" Molecules 27, no. 14: 4439. https://doi.org/10.3390/molecules27144439