Compound Capecitabine Colon-Targeted Microparticle Prepared by Coaxial Electrospray for Treatment of Colon Tumors
Abstract
:1. Introduction
2. Results
2.1. Formula Ratio
2.2. Preparation and Characterization of Microparticles
2.3. In Vitro Drug Release
2.4. Cellular Growth Inhibition
2.5. In Vivo Antitumor Effects of Microparticles
3. Materials and Methods
3.1. Materials
3.2. Fabrication of the Eudragit S100/CAP-OSI-PLGA Microparticles (COPMP), Eudragit S100/CAP-PLGA Microparticles (CPMP), Eudragit S100/OSI-PLGA Microparticles (OPMP), and Eudragit S100/PLGA Microparticles (PMP)
3.3. Structural Morphology and Size Distribution
3.4. Drug Loading (DL) and Encapsulation Efficiency (EE)
3.5. Physical Characterization
3.6. In Vitro Release
3.7. Cytotoxicity Assay
3.7.1. Formula Ratio
3.7.2. Cellular Growth Inhibition
3.8. In Vivo Antitumor Study
3.9. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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CAP:OSI | IC50,CAP (μM) | IC50,OSI (μM) | CI50 |
---|---|---|---|
Free CAP | 0.6990 | ||
CAP:OSI = 5:1 | 0.1651 | 0.3314 | 0.76 |
CAP:OSI = 2:1 | 0.1771 | 0.2851 | 0.70 |
CAP:OSI = 1:1 | 0.1901 | 0.1901 | 0.57 |
CAP:OSI = 1:2 | 0.3257 | 0.1643 | 0.72 |
CAP:OSI = 1:5 | 0.3571 | 0.1433 | 0.74 |
Free OSI | 0.6373 |
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Chen, R.; Zhai, R.; Wang, C.; Liang, S.; Wang, J.; Liu, Z.; Li, W. Compound Capecitabine Colon-Targeted Microparticle Prepared by Coaxial Electrospray for Treatment of Colon Tumors. Molecules 2022, 27, 5690. https://doi.org/10.3390/molecules27175690
Chen R, Zhai R, Wang C, Liang S, Wang J, Liu Z, Li W. Compound Capecitabine Colon-Targeted Microparticle Prepared by Coaxial Electrospray for Treatment of Colon Tumors. Molecules. 2022; 27(17):5690. https://doi.org/10.3390/molecules27175690
Chicago/Turabian StyleChen, Ruiqi, Ruidong Zhai, Chao Wang, Shulong Liang, Jing Wang, Zhepeng Liu, and Wenlin Li. 2022. "Compound Capecitabine Colon-Targeted Microparticle Prepared by Coaxial Electrospray for Treatment of Colon Tumors" Molecules 27, no. 17: 5690. https://doi.org/10.3390/molecules27175690