Polygonatum sibiricum Polysaccharides Attenuate Lipopoly-Saccharide-Induced Septic Liver Injury by Suppression of Pyroptosis via NLRP3/GSDMD Signals
Abstract
:1. Introduction
2. Results
2.1. PSP Increased Survival Rates of Septic Mice
2.2. PSP Alleviated Acute Liver Injury of Septic Mice
2.3. PSP Suppressed MPO Activity and Inflammatory Cytokine Secretion of Septic Mice
2.4. PSP Ameliorated Hepatocyte Pyroptosis of Septic Mice
2.5. PSP Mitigated the Pyroptosis by Inhibiting NLRP3 Inflammasome Signaling
3. Discussion
4. Materials and Methods
4.1. Animals and Treatments
4.2. Specimen Collection
4.3. Histological Analysis
4.4. TUNEL Assay
4.5. Biochemical Measurements
4.6. Total RNA Extraction and Quantitative RT-PCR Assay
4.7. Western Blot Assay
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Gene | Forward Primer (5′-3′) | Reverse Primer (5′-3′) | Gene ID |
---|---|---|---|
IL-1β | TCGCAGCAGCACATCAACAAGAG | TGCTCATGTCCTCATCCTGGAAGG | NM_008361.4 |
IL-18 | ATGCTTTCTGGACTCCTGCC | AGTCTTCTGACATGGCAGCC | NM_008360.2 |
NLRP3 | GAGCTGGACCTCAGTGACAATGC | ACCAATGCGAGATCCTGACAACAC | NM_001359638.1 |
ASC | GCAACTGCGAGAAGGCTATG | GTGAGCTCCAAGCCATACGA | NM_023258.4 |
Caspase-1 | ACAACCACTCGTACACGTCTTGC | CCAGATCCTCCAGCAGCAACTTC | NM_009807.2 |
β-actin | CCTAGGCACCAGGGTGTGAT | TCCATGTCGTCCCAGTTGGT | NM_007393.5 |
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Xiao, L.; Qi, L.; Zhang, G.; Liu, H.; Gu, Y.; Zhang, L.; Zhang, M.; Wu, H. Polygonatum sibiricum Polysaccharides Attenuate Lipopoly-Saccharide-Induced Septic Liver Injury by Suppression of Pyroptosis via NLRP3/GSDMD Signals. Molecules 2022, 27, 5999. https://doi.org/10.3390/molecules27185999
Xiao L, Qi L, Zhang G, Liu H, Gu Y, Zhang L, Zhang M, Wu H. Polygonatum sibiricum Polysaccharides Attenuate Lipopoly-Saccharide-Induced Septic Liver Injury by Suppression of Pyroptosis via NLRP3/GSDMD Signals. Molecules. 2022; 27(18):5999. https://doi.org/10.3390/molecules27185999
Chicago/Turabian StyleXiao, Linxia, Liang Qi, Guozhe Zhang, Hongxia Liu, Yaqin Gu, Lihu Zhang, Mingguang Zhang, and Hongyan Wu. 2022. "Polygonatum sibiricum Polysaccharides Attenuate Lipopoly-Saccharide-Induced Septic Liver Injury by Suppression of Pyroptosis via NLRP3/GSDMD Signals" Molecules 27, no. 18: 5999. https://doi.org/10.3390/molecules27185999