Isolation of Mirificin and Other Bioactive Isoflavone Glycosides from the Kudzu Root Lyophilisate Using Centrifugal Partition and Flash Chromatographic Techniques
Abstract
:1. Introduction
2. Results and Discussion
2.1. Qualitative and Quantitative Analysis of Isoflavone Constituents of the Kudzu Root Lyophilisate
2.2. Purification and Concentration of the Polar Fraction of Isoflavones Using CPC
2.2.1. Selection and Optimization of the Biphasic Solvent System in CPC
2.2.2. Effectiveness of the CPC Method in Purification and Isolation of Polar Fraction of Kudzu Isoflavone Glycosides
2.3. Isolation of Mirificin and Related Polar Isoflavone Glycosides Using Flash Chromatography
2.4. Graphical Presentation of Preparative Extraction and Chromatographic Procedures
2.5. Molecular Structure Analysis of the Obtained Isoflavone Isolates Using Advanced Spectrometric and Spectroscopic Techniques
3. Materials and Methods
3.1. Solvents and Other Reagents
3.2. Plant Material and Obtaining a Vacuum-Dried Kudzu Root Extract
3.3. Centrifugal Partition Chromatography (CPC)
3.3.1. Determination of Partition Coefficients for Various Solvent Systems
3.3.2. Apparatus and Chromatographic Procedure
3.4. Flash Chromatography (FC)
3.5. RP-LC/PDA Protocol
3.6. Mass Spectrometry (MS) and Nuclear Magnetic Resonance (NMR)
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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No | Biphasic Solvent System /Solvent Composition in Volume Proportions | KD |
---|---|---|
1. | EtOAc-BuOH-H2O/1:1:1.5 | 1.99 |
2. | EtOAc-BuOH-H2O/1:1:2 | 1.56 |
3. | EtOAc-BuOH-H2O/1:1:1 | 1.35 |
4. | EtOAc-EtOH-H2O/5:1:5 | 1.01 |
5. | EtOAc-EtOH-H2O/5:2:4 | 0.96 |
6. | EtOAc-EtOH-H2O/10:1:10 + 1% (V/V) CH3COOH | 0.42 |
7. | EtOAc-EtOH-H2O/10:1:10 + 0.5% (V/V) CH3COOH | 0.36 |
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Maciejewska-Turska, M.; Pecio, Ł.; Zgórka, G. Isolation of Mirificin and Other Bioactive Isoflavone Glycosides from the Kudzu Root Lyophilisate Using Centrifugal Partition and Flash Chromatographic Techniques. Molecules 2022, 27, 6227. https://doi.org/10.3390/molecules27196227
Maciejewska-Turska M, Pecio Ł, Zgórka G. Isolation of Mirificin and Other Bioactive Isoflavone Glycosides from the Kudzu Root Lyophilisate Using Centrifugal Partition and Flash Chromatographic Techniques. Molecules. 2022; 27(19):6227. https://doi.org/10.3390/molecules27196227
Chicago/Turabian StyleMaciejewska-Turska, Magdalena, Łukasz Pecio, and Grażyna Zgórka. 2022. "Isolation of Mirificin and Other Bioactive Isoflavone Glycosides from the Kudzu Root Lyophilisate Using Centrifugal Partition and Flash Chromatographic Techniques" Molecules 27, no. 19: 6227. https://doi.org/10.3390/molecules27196227