Capric Acid Behaves Agonistic Effect on Calcitriol to Control Inflammatory Mediators in Colon Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. Effect of CA and Cal on Virtual Binding of Apoptotic and Inflammatory Markers COX-2 and NF-κB
2.2. Antiproliferative Effect of CA and Cal on HCT116 Cancer Cell Lines
2.3. Effect of CA and Cal on LPS-Induced HCT116 Cancer Cell Lines
2.4. Synergistic Effect of CA and Cal on Cell Migration, Invasion and Sphere Formation Properties of HCT116
2.5. Synergistic Effect of CA and Cal on MMP-2 and MMP-9 Inhibition in LPS-Stimulated HCT116 Tumorigenic Cells
2.6. Synergistic Effect of CA and Cal Inhibit Inflammation in HCT116 via COX-2/ NF-κB Pathway
3. Discussion
4. Materials and Methods
4.1. Ligand Preparation and In Silico Docking Analysis
4.2. Cell Culture and MTT Assay
4.3. In Vitro Wound-Healing Assay (Scratch Wound Method)
4.4. Matrigel Invasion Assay
4.5. Spheroid Formation Assay
4.6. Zymographic Assays for MMP-2 and MMP-9
4.7. Effect of CA and Cal on Inflammatory mRNA Mediators of HCT116
4.8. Effect of CA and Cal on Inflammatory Protein Mediators of HCT116
4.9. COX-2 and NF-κB Immunofluorescence
4.10. Statistics Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Negm, A.; Sedky, A.; Elsawy, H. Capric Acid Behaves Agonistic Effect on Calcitriol to Control Inflammatory Mediators in Colon Cancer Cells. Molecules 2022, 27, 6624. https://doi.org/10.3390/molecules27196624
Negm A, Sedky A, Elsawy H. Capric Acid Behaves Agonistic Effect on Calcitriol to Control Inflammatory Mediators in Colon Cancer Cells. Molecules. 2022; 27(19):6624. https://doi.org/10.3390/molecules27196624
Chicago/Turabian StyleNegm, Amr, Azza Sedky, and Hany Elsawy. 2022. "Capric Acid Behaves Agonistic Effect on Calcitriol to Control Inflammatory Mediators in Colon Cancer Cells" Molecules 27, no. 19: 6624. https://doi.org/10.3390/molecules27196624