Synthesis and Biological Evaluation of PEGylated MWO4 Nanoparticles as Sonodynamic AID Inhibitors in Treating Diffuse Large B-Cell Lymphoma
Abstract
:1. Introduction
2. Results
2.1. Synthesis and Characterization of Small Molecule Inhibitors to AID
2.2. Molecular Dynamic Stimulation of the Interaction between ROS and AID
2.3. Application of MWO4-PEG NPs to Treat DLBCL
2.4. Inhibition of MWO4-PEG NPs to AID in DLBCL
2.5. Sonosensitivity and Toxicity of MWO4-PEG NPs
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Materials
5.2. Cell Culture and Construction of Stable Clones
5.3. Molecular Dynamics
5.4. Synthesis of MWO4 (M = Fe Mn Co Ni) Nanoparticles
5.5. Measurement of ROS Generation
5.6. Detection of Cell Apoptosis
5.7. Western Blotting
5.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Jiao, J.; Qian, Z.; Wang, Y.; Liu, M.; Fan, L.; Liu, M.; Hao, Z.; Jiao, J.; Lv, Z. Synthesis and Biological Evaluation of PEGylated MWO4 Nanoparticles as Sonodynamic AID Inhibitors in Treating Diffuse Large B-Cell Lymphoma. Molecules 2022, 27, 7143. https://doi.org/10.3390/molecules27217143
Jiao J, Qian Z, Wang Y, Liu M, Fan L, Liu M, Hao Z, Jiao J, Lv Z. Synthesis and Biological Evaluation of PEGylated MWO4 Nanoparticles as Sonodynamic AID Inhibitors in Treating Diffuse Large B-Cell Lymphoma. Molecules. 2022; 27(21):7143. https://doi.org/10.3390/molecules27217143
Chicago/Turabian StyleJiao, Junna, Zhuang Qian, Yurong Wang, Mei Liu, Liye Fan, Mengqing Liu, Zichen Hao, Junrong Jiao, and Zhuangwei Lv. 2022. "Synthesis and Biological Evaluation of PEGylated MWO4 Nanoparticles as Sonodynamic AID Inhibitors in Treating Diffuse Large B-Cell Lymphoma" Molecules 27, no. 21: 7143. https://doi.org/10.3390/molecules27217143