Purification Process and In Vitro and In Vivo Bioactivity Evaluation of Pectolinarin and Linarin from Cirsium japonicum

Round 1

Reviewer 1 Report

This manuscript was well-written and suitable for publication in molecules. The MS quality will benefit from minor corrections, appended below.

-       Why was water used for the extraction of flavonoids in this study?

-       TFC method showed be appropriately referenced

-       In my opinion, when selecting a resin, a higher desorption rate of resin shouldn't just supersede the adsorption capacity. Moreover, Statistical analyses should be included. As I can see, NKA-9 and AB-8resin show similar D_r with D101. In fact, AB-8 could have been a better choice. More explanation should be provided!

 

-       Authors should check the files uploaded (Supplementary and nonpublished files were duplicated)

-       State the nmb replications used for experimental determinations

-       Improve Keywords

 

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Comments for author File: Comments.pdf

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

The authors evaluated "Purification process and in vitro and in vivo bioactivity evaluation of pectolinarin and linarin from Cirsium japonicum". Overall, the topic is interesting and time relevant. However, the main concern to accept this manuscript for publication is mentioned in the pdf file as attached.

Comments for author File: Comments.pdf

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

The authors revised satisfactorily

Author Response

Thanks a lot for your comments and patience. Your constructive comments have helped us greatly to improve our manuscript.