Antiproliferative Evaluation of Novel 4-Imidazolidinone Derivatives as Anticancer Agent Which Triggers ROS-Dependent Apoptosis in Colorectal Cancer Cell
Abstract
:1. Introduction
2. Results
2.1. Anticancer Activity Evaluation of Compounds 9
2.2. Compound 9r Induces Apoptosis
2.3. Compound 9r Triggers Mitochondrial Apoptosis
2.4. Compound 9r Induces Caspase-Dependent Apoptosis
2.5. Compound 9r Induces ROS Production
2.6. Compound 9r Induces Apoptosis through ROS Generation Which Activates JNK Pathway
3. Discussion
4. Materials and Methods
4.1. Chemical Synthesis of Compounds 9
4.2. Cell Culture
4.3. Reagents and Antibodies
4.4. MTT Assay
4.5. Colony Formation Assay
4.6. DAPI/PI Staining Assay
4.7. Apoptosis Analysis
4.8. Mitochondrial Membrane Potential Assay
4.9. Measurement of Intracellular ROS
4.10. Western Blotting
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Huang, J.; Wang, J.; Song, G.; Hu, C.; Xu, Z.; Chen, Z.; Xu, C.; Yang, D. Antiproliferative Evaluation of Novel 4-Imidazolidinone Derivatives as Anticancer Agent Which Triggers ROS-Dependent Apoptosis in Colorectal Cancer Cell. Molecules 2022, 27, 8844. https://doi.org/10.3390/molecules27248844
Huang J, Wang J, Song G, Hu C, Xu Z, Chen Z, Xu C, Yang D. Antiproliferative Evaluation of Novel 4-Imidazolidinone Derivatives as Anticancer Agent Which Triggers ROS-Dependent Apoptosis in Colorectal Cancer Cell. Molecules. 2022; 27(24):8844. https://doi.org/10.3390/molecules27248844
Chicago/Turabian StyleHuang, Jiuhong, Juanli Wang, Guiting Song, Chunsheng Hu, Zhigang Xu, Zhongzhu Chen, Chuan Xu, and Donglin Yang. 2022. "Antiproliferative Evaluation of Novel 4-Imidazolidinone Derivatives as Anticancer Agent Which Triggers ROS-Dependent Apoptosis in Colorectal Cancer Cell" Molecules 27, no. 24: 8844. https://doi.org/10.3390/molecules27248844