Flavonoids: Antiplatelet Effect as Inhibitors of COX-1
Abstract
:1. Introduction
2. Results
2.1. Studies of Antiaggregant Activity by Impedance Platelet Aggregometry
2.2. Studies of COX Activity by Measuring MDA Production
2.3. Study on Inhibition of Pure Human Recombinant Enzyme
2.4. TXB2 Levels as COX-1 Activity Indicator
3. Discussion
4. Materials and Methods
4.1. Materials
4.1.1. Selected Drugs
4.1.2. Volunteers
4.1.3. Ethical Approval
4.2. Blood Draws and Settings
4.2.1. WB Samples
4.2.2. PRP Samples
4.3. Impedance Aggregometry
4.4. Lipid Peroxidation Measurement
4.5. Study on Inhibition of Pure Human Recombinant Enzyme
4.6. Enzyme Immunoassay for the Quantitative Determination of TXB2
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
Abbreviations
AA | arachidonic acid |
ADP | adenosine diphosphate |
ATA | trichloroacetic |
CI | calcium ionophore |
COX | cyclooxygenase |
DMSO | dimethyl sulfoxide |
EU | enzyme unit |
GPIIb/IIIa | glycoprotein IIb/IIIa |
h-COX-1 | human recombinant enzyme |
MAPK/ERK1/2 | mitogen-activated protein kinases/extracellular signal-regulated kinase 1/2 |
MDA | malondialdehyde |
PG | prostaglandin |
PGG2 | prostaglandin G2 |
PGH2 | prostaglandin H2 |
PI3K/Akt/mTOR | phosphatidylinositol-3-kinase/serine/threonine kinase/mammalian target of rapamycin |
PRP | platelet-rich plasma |
PSS | physiological saline solution |
rpm | revolution per minute |
TBA | tiobarbituric acid |
TMPD | N,N,N′,N′-tetramethyl-p-phenylenediamine |
TXA2 | thromboxane A2 |
TXB2 | thromboxane B2 |
WB | whole blood |
SD | standard deviation |
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Zaragozá, C.; Álvarez-Mon, M.Á.; Zaragozá, F.; Villaescusa, L. Flavonoids: Antiplatelet Effect as Inhibitors of COX-1. Molecules 2022, 27, 1146. https://doi.org/10.3390/molecules27031146
Zaragozá C, Álvarez-Mon MÁ, Zaragozá F, Villaescusa L. Flavonoids: Antiplatelet Effect as Inhibitors of COX-1. Molecules. 2022; 27(3):1146. https://doi.org/10.3390/molecules27031146
Chicago/Turabian StyleZaragozá, Cristina, Miguel Ángel Álvarez-Mon, Francisco Zaragozá, and Lucinda Villaescusa. 2022. "Flavonoids: Antiplatelet Effect as Inhibitors of COX-1" Molecules 27, no. 3: 1146. https://doi.org/10.3390/molecules27031146