Molecular Pathological Diagnosis of Thyroid Tumors Using Spatially Resolved Metabolomics
Abstract
:1. Introduction
2. Results
2.1. Spatially Resolved Metabolic Profiling
2.2. Biomarkers for Discriminating Benign FA from Malignant PTC
2.3. Biomarkers for Discriminating Benign FA from Malignant FTC
2.4. Diagnostic Model and Discriminant Analysis
3. Discussion
4. Materials and Methods
4.1. Sample Collection and Preparation
4.2. AFADESI-MSI Experiment
4.3. Data Processing
4.4. Metabolite Identification
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Positive Mode | Negative Mode | Computational Prediction | ||||
---|---|---|---|---|---|---|
Class FA | Class FTC | Class FA | Class FTC | Class FA | Class FTC | |
TC13-P-1-A | 0.9262 | 0.0738 | 0.6859 | 0.3141 | 0.8060 | 0.1940 |
TC13-P-1-B | 0.9632 | 0.0368 | 0.7837 | 0.2163 | 0.8735 | 0.1265 |
TC13-P-1-C | 0.9195 | 0.0805 | 0.8438 | 0.1562 | 0.8817 | 0.1183 |
TC13-P-1-D | 0.8737 | 0.1263 | 0.7340 | 0.2660 | 0.8038 | 0.1962 |
TC13-P-2-E | 0.9160 | 0.0840 | 0.5535 | 0.4465 | 0.7347 | 0.2653 |
TC13-P-2-F | 0.7397 | 0.2603 | 0.7103 | 0.2897 | 0.7250 | 0.2750 |
TC13-P-2-G | 0.9898 | 0.0102 | 0.7180 | 0.2820 | 0.8539 | 0.1461 |
TC13-P-2-H | 1.5810 | −0.5810 | 0.6761 | 0.3239 | 1.1286 | −0.1286 |
TC13-P-3-I | 0.8098 | 0.1902 | 0.7215 | 0.2785 | 0.7656 | 0.2344 |
TC13-P-3-J | 0.8937 | 0.1063 | 0.8215 | 0.1785 | 0.8576 | 0.1424 |
TC13-P-3-K | 0.9020 | 0.0980 | 0.8260 | 0.1740 | 0.8640 | 0.1360 |
TC13-P-3-L | 0.8442 | 0.1558 | 0.7925 | 0.2075 | 0.8183 | 0.1817 |
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Huang, L.; Mao, X.; Sun, C.; Li, T.; Song, X.; Li, J.; Gao, S.; Zhang, R.; Chen, J.; He, J.; et al. Molecular Pathological Diagnosis of Thyroid Tumors Using Spatially Resolved Metabolomics. Molecules 2022, 27, 1390. https://doi.org/10.3390/molecules27041390
Huang L, Mao X, Sun C, Li T, Song X, Li J, Gao S, Zhang R, Chen J, He J, et al. Molecular Pathological Diagnosis of Thyroid Tumors Using Spatially Resolved Metabolomics. Molecules. 2022; 27(4):1390. https://doi.org/10.3390/molecules27041390
Chicago/Turabian StyleHuang, Luojiao, Xinxin Mao, Chenglong Sun, Tiegang Li, Xiaowei Song, Jiangshuo Li, Shanshan Gao, Ruiping Zhang, Jie Chen, Jiuming He, and et al. 2022. "Molecular Pathological Diagnosis of Thyroid Tumors Using Spatially Resolved Metabolomics" Molecules 27, no. 4: 1390. https://doi.org/10.3390/molecules27041390