Identification of Antibacterial Sterols from Korean Wild Mushroom Daedaleopsis confragosa via Bioactivity- and LC-MS/MS Profile-Guided Fractionation
Abstract
:1. Introduction
2. Results and Discussion
2.1. Extraction of Metabolites from D. confragosa and Bioactivity-Guided Fractionation
2.2. LC–MS/MS Analysis of the Extract and Fractions
2.3. Isolation and Chemical Characterization of the Compounds
2.4. Evaluation of Antibacterial Activity of the Isolated Compounds against H. pylori
2.5. Distribution Analysis of Compound 1 in D. confragosa and Other Fungi
3. Materials and Methods
3.1. General Experimental Procedure
3.2. Mushroom Material
3.3. Extraction of D. Confragosa and Solvent Partition
3.4. LC–MS/MS Analysis
3.5. Isolation of Compounds from the Hexane and CH2Cl2 Fractions
3.6. Helicobacter Pylori Culture
3.7. Anti-Helicobacter Pylori Activity
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Sample | Concentrations | Inhibition (%) |
---|---|---|
Methanolic extract | 100 μg/mL | 22.4 |
n-hexane fraction | 38.2 | |
CH2Cl2 fraction | 23.3 | |
EtOAc fraction | 16.0 | |
BuOH fraction | 15.6 | |
Quercetin a | 100 μM | 22.2 |
Metronidazole a | 73.5 |
Compound | Concentrations | Inhibition (%) |
---|---|---|
1 | 100 μM | 33.9 |
2 | 8.6 | |
3 | 18.5 | |
4 | 6.8 | |
5 | 3.4 | |
Quercetin a | 100 μM | 22.2 |
Metronidazole a | 73.5 |
Dataset | Species | Family |
---|---|---|
MSV000084977 | Fusarium graminearum | Nectraiaceae |
MSV000085071 | Leucoagaricus atrodisca | Agaricaceae |
Lepiota spheniscuspora | ||
Amanita pantherinoides | Amanitaceae | |
Amanita protecta | ||
Phylloporus arenicola | Boletaceae | |
Suillelus amygdalinus | ||
Bondarzewia occidentalis | Bondarzewiaceae | |
Cantharellus californicus | Cantharellaceae | |
Cantharellus subalbidus | ||
Craterellus tubaeformis | ||
Clavariadelphus mucronatus | Clavariadelphaceae | |
Clavariadelphus occidentalis | ||
Clavulina cinerea | Clavulinaceae | |
Cortinarius alboglobosus | Cortinariaceae | |
Cortinarius albogfragrans | ||
Cortinarius alboviolaceus | ||
Cortinarius anomalus | ||
Cortinarius cinnamomeus | ||
Cortinarius collinitus | ||
Cortinarius caperatus | ||
Cortinarius causticus | ||
Cortinarius croceus | ||
Clitopilus hobsonii | Entolomataceae | |
Gomphus vlavatus | Gomphaceae | |
Ramaria araispora var. araiospora | ||
Ramaria formosa | ||
Ramaria gelatonosa var. oregonensis | ||
Ramaria rubripermanens | ||
Ramaria sandaracina var. sandaracina | ||
Hydnum umbilicatum | Hydnaceae | |
Armillaria solidipes | Physalacriaceae | |
Polyporus leptocephalus | Polyporaceae | |
Trametes hirsuta | ||
Trametes versicolor | ||
Coprinopsis atramentaria | Psathyrellaceae | |
Lactarius aestivus | Russulaceae | |
Lactarius rubrilacteus | ||
Lactarius scrobiculatus | ||
Russula americana | ||
Russula xerampelina | ||
Pholiota flammans | Strophariaceae | |
Clitocybe nuda | Tricholomataceae | |
Collybia cirrhata | ||
Leucopaxillus albissimus | ||
Tricholoma inamoenum | ||
Tricholoma mutabile | ||
Tricholoma pardinum | ||
Tricholoma portentosum | ||
MSV000085974 | Cerrena unicolor | Polyporaceae |
Coriolopsis strumose | ||
Daedaleopsis tricolor | ||
Microporus vernicipes | ||
Polyporus brumalis | ||
Trametes orientalis | ||
Trametes suaveolens | ||
Trametopsis cervina |
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Na, M.W.; Lee, E.; Kang, D.-M.; Jeong, S.Y.; Ryoo, R.; Kim, C.-Y.; Ahn, M.-J.; Kang, K.B.; Kim, K.H. Identification of Antibacterial Sterols from Korean Wild Mushroom Daedaleopsis confragosa via Bioactivity- and LC-MS/MS Profile-Guided Fractionation. Molecules 2022, 27, 1865. https://doi.org/10.3390/molecules27061865
Na MW, Lee E, Kang D-M, Jeong SY, Ryoo R, Kim C-Y, Ahn M-J, Kang KB, Kim KH. Identification of Antibacterial Sterols from Korean Wild Mushroom Daedaleopsis confragosa via Bioactivity- and LC-MS/MS Profile-Guided Fractionation. Molecules. 2022; 27(6):1865. https://doi.org/10.3390/molecules27061865
Chicago/Turabian StyleNa, Myung Woo, Eunjin Lee, Dong-Min Kang, Se Yun Jeong, Rhim Ryoo, Chul-Young Kim, Mi-Jeong Ahn, Kyo Bin Kang, and Ki Hyun Kim. 2022. "Identification of Antibacterial Sterols from Korean Wild Mushroom Daedaleopsis confragosa via Bioactivity- and LC-MS/MS Profile-Guided Fractionation" Molecules 27, no. 6: 1865. https://doi.org/10.3390/molecules27061865