Comparison of the Content of Several Elements in Seawater, Sea Cucumber Eupentacta fraudatrix and Its High-Molecular-Mass Multiprotein Complex
Abstract
:1. Introduction
2. Results
2.1. Seawater, Cucumbers, and Multi-Protein Complex
2.2. Determination of Elements by TJP-AES
3. Discussion
3.1. The Content of Metal Ions in Sea Cucumber
3.2. Biological Role of Different Metal Ions
4. Materials and Methods
4.1. Reagents
4.2. Purification of Stable Complexes by Gel Filtration
4.3. Multi-Elemental Analysis of the Samples
4.4. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
- U.S. National Library of Medicine; National Institutes of Health; U.S. Department of Health & Human Services. Graphic Courtesy of the Scientific Consulting Group. Available online: nlm.nih.gov (accessed on 20 February 2018).
- Cicero, C.E.; Mostile, G.; Vasta, R.; Rapisarda, V.; Signorelli, S.S.; Ferrante, M.; Zappia, M.; Nicoletti, A. Metals and neurodegenerative diseases. A systematic review. Environ. Res. 2017, 159, 82–94. [Google Scholar] [CrossRef] [PubMed]
- Seneviratne, M.; Rajakaruna, N.; Rizwan, M.; Madawala, H.M.S.P.; Ok, Y.S.; Vithanage, M. Heavy metal-induced oxidative stress on seed germination and seedling development: A critical review. Environ. Geochem. Health 2017, 39, 1421–1439. [Google Scholar]
- Bargagli, E.; Lavorini, F.; Pistolesi, M.; Rosi, E.; Prasse, A.; Rota, E.; Voltolini, L. Trace metals in fluids lining the respiratory system of patients with idiopathic pulmonary fibrosis and diffuse lung diseases. J. Trace Elem. Med. Biol. 2017, 42, 39–44. [Google Scholar] [CrossRef] [PubMed]
- Mohammadifard, N.; Humphries, K.H.; Gotay, C.; Mena-Sánchez, G.; Salas-Salvadó, J.; Esmaillzadeh, A.; Ignaszewski, A.; Sarrafzadegan, N. Trace minerals intake: Risks and benefits for cardiovascular health. Crit. Rev. Food Sci. Nutr. 2017, 13, 1–13. [Google Scholar] [CrossRef]
- Homoky, W.B.; Weber, T.; Berelson, W.M.; Conway, T.M.; Henderson, G.M.; van Hulten, M.; Jeandel, C.; Severmann, S.; Tagliabue, A. Quantifying trace element and isotope fluxes at the ocean-sediment boundary: A review. Philos. Trans A Math. Phys. Eng. Sci. 2016, 374, 2081. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Taylor, A.; Day, M.P.; Hill, S.; Marshall, J.; Patriarca, M.; White, M. Atomic spectrometry update: Review of advances in the analysis of clinical and biological materials, foods and beverages. J. Anal. Spectrom. 2015, 30, 542–579. [Google Scholar] [CrossRef] [Green Version]
- Taylor, A.; Barlow, N.; Day, M.P.; Hill, S.; Patriarca, M.; White, M. Atomic spectrometry update: Review of advances in the analysis of clinical and biological materials, foods and beverages. J. Anal. Spectrom. 2017, 32, 432–476. [Google Scholar] [CrossRef]
- Zheenbaev, Z.Z.; Engelsht, V.S. Dvukhstruinyi Plazmatron (Two-Jet Plasmatron); Frunze: Ilim, Russia, 1983; 200p. [Google Scholar]
- Yudelevich, I.G.; Cherevko, A.S.; Engelsht, V.S.; Pikalov, V.V.; Tagiltsev, A.P.; Zheenbaev, Z.Z. A two-jet plasmatron for the spectrochemical analysis of geological samples. Spectrochim. Acta Part B 1984, 39, 777–785. [Google Scholar] [CrossRef]
- Zaksas, N.P. Solid Sampling in Analysis of Various Plants Using Two-Jet Plasma Atomic Emission Spectrometry. Appl. Spectrosc. 2019, 73, 870–878. [Google Scholar] [CrossRef]
- Zaksas, N.P.; Sultangazieva, T.T.; Korda, T.M. Using a two-jet arc plasmatron for determining the trace element composition of powdered biological samples. J. Anal. Chem. 2006, 61, 632–637. [Google Scholar] [CrossRef]
- Zaksas, N.P.; Nevinsky, G.A. Solid sampling in analysis of animal organs by two-jet plasma atomic emission spectrometry. Spectrochim. Acta Part B 2011, 66, 861–865. [Google Scholar] [CrossRef]
- Zaksas, N.P.; Gerasimov, V.A.; Nevinsky, G.A. Simultaneous determination of Fe, P, Ca, Mg, Zn and Cu in whole blood by two-jet plasma atomic emission spectrometry. Talanta 2010, 80, 2187–2190. [Google Scholar] [CrossRef]
- Zaksas, N.P.; Sultangazieva, T.T.; Gerasimov, V.A. Determination of trace elements in bone by two-jet plasma atomic emission spectrometry. Anal. Bioanal. Chem. 2008, 391, 687–693. [Google Scholar] [CrossRef] [PubMed]
- Zaksas, N.; Gluhcheva, Y.; Sedykh, S.; Madzharova, M.; Atanassova, N.; Nevinsky, G. Effect of CoCl(2) treatment on major and trace elements metabolism and protein concentration in mice. J. Trace Elem. Med. Biol. 2013, 27, 27–30. [Google Scholar] [CrossRef] [PubMed]
- Tolmacheva, A.S.; Zaksas, N.P.; Buneva, V.N.; Vasilenko, N.L.; Nevinsky, G.A. Oxidoreductase activities of polyclonal IgG from the sera of Wistar rats are better activated by combinations of different metal ions. J. Mol. Recognit. 2009, 22, 26–37. [Google Scholar] [CrossRef]
- Soboleva, S.E.; Zaksas, N.P.; Nevinsky, G.A. Comparison of Trace Elements in High-Molecular-Mass Multiprotein Complex and in Female Milk from Which It Was Obtained. Sci. World J. 2019, 2019, 9782635. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Alberts, B. The cell as a collection of protein machines: Preparing the next generation of molecular biologists. Cell 1998, 92, 291–294. [Google Scholar] [CrossRef] [Green Version]
- Eubel, H.; Braun, H.P.; Millar, A.H. Blue-native PAGE in plants: A tool in analysis of protein-protein interactions. Plant Methods 2005, 1, 11. [Google Scholar] [CrossRef] [Green Version]
- Soboleva, S.E.; Dmitrenok, P.S.; Verkhovod, T.D.; Buneva, V.N.; Sedykh, S.E.; Nevinsky, G.A. Very stable high molecular mass multiprotein complex with DNase and amylase activities in human milk. J. Mol. Recognit. 2015, 28, 20–34. [Google Scholar] [CrossRef]
- Burkova, E.E.; Dmitrenok, P.S.; Sedykh, S.E.; Buneva, V.N.; Soboleva, S.E.; Nevinsky, G.A. Extremely stable soluble high molecular mass multi-protein complex with DNase activity in human placental tissue. PLoS ONE 2014, 9, e111234. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Burkova, E.E.; Dmitrenok, P.S.; Bulgakov, D.V.; Ermakov, E.A.; Buneva, V.N.; Soboleva, S.E.; Nevinsky, G.A. Identification of major proteins of a very stable high molecular mass multi-protein complex of human placental tissue possessing nine different catalytic activities. Biochem. Anal. Biochem. 2018, 7, 351. [Google Scholar] [CrossRef]
- Soboleva, S.E.; Burkova, E.E.; Dmitrenok, P.S.; Bulgakov, D.V.; Menzorova, N.I.; Buneva, V.N.; Nevinsky, G.A. Extremely stable high molecular mass soluble multiprotein complex from eggs of sea urchin Strongylocentrotus intermedius with phosphatase activity. J. Mol. Recognit. 2018, 31, e2753. [Google Scholar] [CrossRef] [PubMed]
- Timofeeva, A.M.; Kostrikina, I.A.; Dmitrenok, P.S.; Soboleva, S.E.; Nevinsky, G.A. Very Stable Two Mega Dalton High-Molecular-Mass Multiprotein Complex from Sea Cucumber Eupentacta fraudatrix. Molecules 2021, 26, 5703. [Google Scholar] [CrossRef] [PubMed]
- Anspaugh, L.R.; Robison, W.L. Trace elements in biology and medicine. Prog. Med. 1971, 3, 63–138. [Google Scholar]
- Lakatos, B.; Szentmihályi, K.; Vinkler, P.; Balla, J.; Balla, G.O. The role of essential metal ions in the human organism and their oral supplementation to the human body in deficiency states, Review. Orv. Hetil. 2004, 145, 1315–1319. [Google Scholar] [PubMed]
- Dubina, T.L.; Leonov, V.A. Metals in the body and their role in the processes of aging. Usp. Sovrem. Biol. 1968, 66, 453–470. [Google Scholar] [PubMed]
- Forssén, A. Inorganic elements in the human body. I. Occurrence of Ba, Br, Ca, Cd, Cs, Cu, K, Mn, Ni, Sn, Sr, Y and Zn in the human body. Ann. Med. Exp. Biol. Fenn. 1972, 50, 99–162. [Google Scholar] [PubMed]
- Aggett, P.J. Physiology and metabolism of essential trace elements: An outline. Clin. Endocrinol. Metab. 1985, 14, 513–543. [Google Scholar] [CrossRef]
- Graham, R.D.; Stangoulis, J.C. Trace element uptake and distribution in plants. J. Nutr. 2003, 133, 1502S–1505S. [Google Scholar] [CrossRef] [Green Version]
- Uluisik, I.; Karakaya, H.C.; Koc, A.J. The importance of boron in biological systems. Trace Elem. Med. Biol. 2018, 45, 156–162. [Google Scholar] [CrossRef] [PubMed]
- Tejada-Jiménez, M.; Chamizo-Ampudia, A.; Galván, A.; Fernández, E.; Llamas, Á. Molybdenum metabolism in plants. Metallomics 2013, 5, 1191–1203. [Google Scholar] [CrossRef]
- Broadley, M.R.; White, P.J.; Hammond, J.P.; Zelko, I.; Lux, A. Zinc in plants. New Phytol. 2007, 173, 677–702. [Google Scholar] [CrossRef] [PubMed]
- Burkhead, J.L.; Gogolin Reynolds, K.A.; Abdel-Ghany, S.E.; Cohu, C.M.; Pilon, M. Copper homeostasis. New Phytol. 2009, 182, 799–816. [Google Scholar] [CrossRef] [PubMed]
- Li, J.; Jia, Y.; Dong, R.; Huang, R.; Liu, P.; Li, X.; Wang, Z.; Liu, G.; Chen, Z. Advances in the Mechanisms of Plant Tolerance to Manganese Toxicity. Review. Int. J. Mol. Sci. 2019, 20, 5096. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Malea, P.; Kevrekidis, T. Trace element patterns in marine macroalgae. Sci. Total Environ. 2014, 494–495, 144–157. [Google Scholar] [CrossRef] [PubMed]
- Malea, P.; Kevrekidis, T. Trace element (Al, As, B, Ba, Cr, Mo, Ni, Se, Sr, Tl, U and V) distribution and seasonality in compartments of the seagrass Cymodocea nodosa. Sci. Total Environ. 2013, 463–464, 611–623. [Google Scholar] [CrossRef] [PubMed]
- Bonanno, G.; Orlando-Bonaca, M. Trace elements in Mediterranean seagrasses and macroalgae. A review. Sci. Total Environ. 2018, 618, 1152–1159. [Google Scholar] [CrossRef]
- Pors, N.S. The biological role of strontium. Bone 2004, 35, 583–588. [Google Scholar] [CrossRef]
- Vincent, J.B. Elucidating a biological role for chromium at a molecular level. Acc. Chem. Res. 2000, 33, 503–510. [Google Scholar] [CrossRef]
- Losi, M.E.; Amrhein, C.; Frankenberger, W.T. Environmental biochemistry of chromium. Rev. Environ. Contam. Toxicol. 1994, 136, 91–121. [Google Scholar]
- Carlisle, E.M. Silicon as an essential trace element in animal nutrition. Ciba Found Symp. 1986, 121, 123–139. [Google Scholar]
- Tanaka, M.; Fujiwara, T. Physiological roles and transport mechanisms of boron: Perspectives from plants. Review. Pflugers Arch. 2008, 456, 671–677. [Google Scholar] [CrossRef] [PubMed]
- Carrano, C.J.; Schellenberg, S.; Amin, S.A.; Green, D.H.; Küpper, F.C. Boron and marine life: A new look at an enigmatic bioelement. Mar. Biotechnol. 2009, 11, 431–440. [Google Scholar] [CrossRef] [Green Version]
- Khaliq, H.; Juming, Z.; Ke-Mei, P. The Physiological Role of Boron on Health. Biol. Trace Elem. Res. 2018, 186, 31–51. [Google Scholar] [CrossRef]
- Zhu, W.; Richards, N.G.J. Biological functions controlled by manganese redox changes in mononuclear Mn-dependent enzymes. Essays Biochem. 2017, 61, 259–270. [Google Scholar] [PubMed] [Green Version]
- Thomas, J.W. Metabolism of iron and manganese. J. Dairy Sci. 1970, 53, 1107–1123. [Google Scholar] [CrossRef]
- Ingrao, G.; Santaroni, G.; Tomassi, G. Trace elements: Biological role and nutritional aspects for humans. Ann. Dell’Istituto Super. Sanità 1995, 31, 275–281. [Google Scholar] [PubMed]
- Boccio, J.; Salgueiro, J.; Lysionek, A.; Zubillaga, M.; Weill, R.; Goldman, C.; Caro, R. Current knowledge of iron metabolism. Biol. Trace Elem. Res. 2003, 92, 189–212. [Google Scholar] [CrossRef]
- Kravchenkom, J.; Darrah, T.H.; Miller, R.K.; Lyerly, H.K.; Vengosh, A. A review of the health impacts of barium from natural and anthropogenic exposure. Environ. Geochem. Health 2014, 36, 797–814. [Google Scholar] [CrossRef] [PubMed]
- Su, J.F.; Le, D.P.; Liu, C.H.; Lin, J.D.; Xiao, X.J. Critical care management of patients with barium poisoning: A case series. Chin. Med. J. 2020, 133, 724–725. [Google Scholar] [CrossRef] [Green Version]
- Frassinetti, S.; Bronzetti, G.; Caltavuturo, L.; Cini, M.; Croce, C.D. The role of zinc in life: A review. J. Environ. Pathol. Toxicol. Oncol. 2006, 25, 597–610. [Google Scholar] [CrossRef]
- Dastych, M. Copper-biochemistry, metabolism and physiologic function. Cas Lek. Cesk. 1997, 136, 670–673. [Google Scholar] [PubMed]
- Exley, C.; Mold, M.J. The binding, transport and fate of aluminium in biological cells. J. Trace Elem. Med. Biol. 2015, 30, 90–95. [Google Scholar] [CrossRef] [PubMed]
- Peto, M.V. Aluminium and iron in humans: Bioaccumulation, pathology, and removal. Rejuvenation Res. 2010, 13, 589–598. [Google Scholar] [CrossRef]
- Page, A.L.; Bingham, F.T. Cadmium residues in the environment. Residue Rev. 1973, 48, 1–44. [Google Scholar] [PubMed]
- Sastry, K.V.; Subhadra, K. Effect of cadmium on some aspects of carbohydrate metabolism in a freshwater catfish Heteropneustes fossilis. Toxicol. Lett. 1982, 14, 45–55. [Google Scholar] [CrossRef]
- Sastry, K.V.; Subhadra, K. In vivo effects of cadmium on some enzyme activities in tissues of the freshwater catfish, Heteropneustes fossilis. Environ. Res. 1985, 36, 32–45. [Google Scholar] [CrossRef]
- Zdrojewicz, Z.; Popowicz, E.; Winiarski, J. Nickel-role in human organism and toxic effects. Pol. Merkur Lekarski. 2016, 41, 115–118. [Google Scholar]
- Zambelli, B.; Ciurli, S. Nickel and human health. Met. Ions Life Sci. 2013, 13, 321–357. [Google Scholar]
- Alexander, J.W. History of the medical use of silver. Surg. Infect. 2009, 10, 289–292. [Google Scholar] [CrossRef] [Green Version]
- Leyssens, L.; Vinck, B.; Van Der Straeten, C.; Wuyts, F.; Maes, L. Cobalt toxicity in humans—A review of the potential sources and systemic health effects. Toxicology 2017, 387, 43–56. [Google Scholar] [CrossRef] [PubMed]
Element | Content of Elements, μg/g | |||||
---|---|---|---|---|---|---|
Seawater | Body of the Sea Cucumber | Stable Protein Complex | Ratio of 2 to 1 | Ratio of 3 to 1 | Ratio of 3 to 2 | |
1 | 2 | 3 | ||||
Si | 1.5 | 63.0 | 180 | 42.0 | 120 | 2.9 |
Sr | 1.7 | 780 | 15.0 | 4.6 × 102 | 8.8 | 0.019 (52.0) |
Cr | 0.13 | 9.7 | 4.1 | 74.6 | 31.5 | 0.42 (2.4) |
B | 5.0 | 61.0 | 4.5 | 13.6 | 0.9 (1.1) | 0.049 (20.3) |
Ca | 350 | 2.5 × 105 | 3200 | 714.3 | 9.1 | 0.0128 (78.1) |
Mg | 930 | 6400 | 44.0 | 6.9 | 0.047 (21.1) | 0.0068 (145.5) |
Mn | n/d (0.005) ** | 26.0 | 1.6 | >5200 | ≥320 | 0.062 (16.3) |
Cu | n/d (0.007) | 1.4 | 7.5 | ≥200 | ≥1071 | 5.4 |
Zn | n/d (0.05) | 13.0 | 51.0 | ≥260 | ≥1020 | 3.9 |
P | n/d (0.5) | 1100 | 290 | ≥2200 | ≥580 | 0.23 (3.8) |
Al | n/d (0.05) | 9.3 | 14.0 | ≥186 | ≥280 | 1.5 |
Ba | n/d (0.05) | 15.0 | 2.5 | ≥300 | ≥50 | 0.17 (6.0) |
Cd | n/d (0.005) | 0.3 | 0.7 | ≥60 | ≥140 | 2.3 |
Fe | n/d (0.05) | 47.0 | 23.0 | ≥940 | ≥460 | 0.49 (2.0) |
Mo | n/d (0.05) | 2.8 | n/d (2.0) | ≥56 | - | - |
Ni | n/d (0.05) | n/d (1.5) | 13.0 | - | ≥260 | - |
Co | n/d (0.03) | n/d (1.2) | 2.0 | - | ≥66.6 | - |
Ag | n/d (0.003) | n/d (0.12) | 0.2 | - | ≥66.6 | - |
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Zaksas, N.P.; Timofeeva, A.M.; Dmitrenok, P.S.; Soboleva, S.E.; Nevinsky, G. Comparison of the Content of Several Elements in Seawater, Sea Cucumber Eupentacta fraudatrix and Its High-Molecular-Mass Multiprotein Complex. Molecules 2022, 27, 1958. https://doi.org/10.3390/molecules27061958
Zaksas NP, Timofeeva AM, Dmitrenok PS, Soboleva SE, Nevinsky G. Comparison of the Content of Several Elements in Seawater, Sea Cucumber Eupentacta fraudatrix and Its High-Molecular-Mass Multiprotein Complex. Molecules. 2022; 27(6):1958. https://doi.org/10.3390/molecules27061958
Chicago/Turabian StyleZaksas, Natalia P., Anna M. Timofeeva, Pavel S. Dmitrenok, Svetlana E. Soboleva, and Georgy Nevinsky. 2022. "Comparison of the Content of Several Elements in Seawater, Sea Cucumber Eupentacta fraudatrix and Its High-Molecular-Mass Multiprotein Complex" Molecules 27, no. 6: 1958. https://doi.org/10.3390/molecules27061958