Prediction of Antibacterial Peptides against Propionibacterium acnes from the Peptidomes of Achatina fulica Mucus Fractions
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of Clindamycin Resistance P. acnes Isolates
2.2. Putative Antibacterial Peptide Screening Using Bioinformatics Prediction
2.3. The Inhibitory Effect of Selected Putative Antibacterial Peptides against P. acnes Isolates with Different Clindamycin Resistance Levels
2.4. The Side Effects of the Original and Modified APAP2-Biomimetic Peptides on Human Fibroblast Cell Line
3. Materials and Methods
3.1. Propionibacterium acnes Isolate Preparation
3.2. Bioinformatic Prediction of Multifunctional Antibacterial Peptides
3.3. Antibacterial Assay
3.4. Side Effect Measurement on Human Skin Fibroblast Cell Line
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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APAP-ID. | Number of Amino Acid Residues | Prediction Scores | |||
---|---|---|---|---|---|
iAMP | dPABBs | ToxinPred | MLCPP | ||
APAP1-original | 7 | 0.73 | 1.23 | −0.91 | 0.73 |
APAP1-modified | 7 | 0.97 | 2.65 | −0.81 | 0.97 |
APAP2-original | 8 | 0.61 | 0.11 | −1.17 | 0.61 |
APAP2-modified | 8 | 0.97 | 2.77 | −1.38 | 0.97 |
APAP3-original | 5 | 0.78 | 0.06 | −0.73 | 0.78 |
APAP3-modified | 5 | 0.97 | 1.88 | −0.83 | 0.97 |
ID | Sequence | Peptide Structure | Physicochemical Properties | |||||
---|---|---|---|---|---|---|---|---|
Hydrophobicity | Hydropathicity | Amphipathicity | Hydrophilicity | Charge | MW | |||
APAP1-original | K RATV YR | | −0.57 | −1.27 | 1.22 | 0.61 | 3 | 893.14 |
APAP1-modified | K RLHV I G | | −0.19 | 0.07 | 1.08 | 0.06 | 2.5 | 822.13 |
APAP2-original | L ATVTV PR | | −0.04 | 0.81 | 0.31 | −0.39 | 1 | 856.14 |
APAP2-modified | L A I V GH K R | | −0.13 | 0.29 | 0.95 | −0.01 | 2.5 | 893.22 |
APAP3-original | V I I AH | | 0.37 | 2.36 | 0.29 | −1.22 | 0.5 | 551.76 |
APAP3-modified | VRIKL | | −0.21 | 0.82 | 1.22 | 0.18 | 2 | 627.9 |
P. acnes Isolates | JB1 | JB9 | JB3 | JB6 | JB7 | JB13 | Mean ± SD | |
---|---|---|---|---|---|---|---|---|
Peptide ID | ||||||||
APAP2-original | 124.346 | 242.24 | 156.29 | 290.82 | 86.6996 | 143.451 | 105.5228 ± 31.41 | |
APAP2-modified | 155.301 | 96.1367 | 55.2911 | 31.7074 | 29.7781 | 24.9624 | 27.37025 ± 20.95 |
Replicates | 1 | 2 | 3 | 4 | Mean | SD | SE | |
---|---|---|---|---|---|---|---|---|
Peptide ID | ||||||||
APAP2-original | 73.89788 | 77.06946 | 90.70726 | 90.3901 | 83.01618 | 8.794591 | 4.397296 | |
APAP2-modified | 101.1735 | 92.29305 | 94.196 | 81.50967 | 92.29305 | 8.139709 | 4.069854 |
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Chalongkulasak, S.; E-kobon, T.; Chumnanpuen, P. Prediction of Antibacterial Peptides against Propionibacterium acnes from the Peptidomes of Achatina fulica Mucus Fractions. Molecules 2022, 27, 2290. https://doi.org/10.3390/molecules27072290
Chalongkulasak S, E-kobon T, Chumnanpuen P. Prediction of Antibacterial Peptides against Propionibacterium acnes from the Peptidomes of Achatina fulica Mucus Fractions. Molecules. 2022; 27(7):2290. https://doi.org/10.3390/molecules27072290
Chicago/Turabian StyleChalongkulasak, Suwapitch, Teerasak E-kobon, and Pramote Chumnanpuen. 2022. "Prediction of Antibacterial Peptides against Propionibacterium acnes from the Peptidomes of Achatina fulica Mucus Fractions" Molecules 27, no. 7: 2290. https://doi.org/10.3390/molecules27072290