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Review

G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling

by
Haoyi Ge
1,2,
Huixia Wang
1,
Benxun Pan
1,2,
Dandan Feng
1,2,
Canyong Guo
1,2,
Lingyun Yang
1,
Dongsheng Liu
1 and
Kurt Wüthrich
1,3,4,*
1
iHuman Institute, ShanghaiTech University, Shanghai 201210, China
2
School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China
3
Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, CA 92037, USA
4
Institute of Molecular Biology and Biophysics, ETH Zürich, Otto-Stern-Weg 5, 8093 Zürich, Switzerland
*
Author to whom correspondence should be addressed.
Molecules 2022, 27(9), 2658; https://doi.org/10.3390/molecules27092658
Submission received: 11 March 2022 / Revised: 14 April 2022 / Accepted: 14 April 2022 / Published: 20 April 2022
(This article belongs to the Special Issue Purinergic Signaling: Targeting GPCRs, Ion Channels and Enzymes—A Theme Issue in Honor of Dr. Kenneth A. Jacobson)
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Abstract

G protein-coupled receptors (GPCRs) are a large membrane protein family found in higher organisms, including the human body. GPCRs mediate cellular responses to diverse extracellular stimuli and thus control key physiological functions, which makes them important targets for drug design. Signaling by GPCRs is related to the structure and dynamics of these proteins, which are modulated by extrinsic ligands as well as by intracellular binding partners such as G proteins and arrestins. Here, we review some basics of using nuclear magnetic resonance (NMR) spectroscopy in solution for the characterization of GPCR conformations and intermolecular interactions that relate to transmembrane signaling.
Keywords: G protein-coupled receptors; 19F-NMR; membrane mimetics; stable-isotope labeling; in-membrane chemical modification; amino-acid-specific NMR labeling; sequence-specific NMR labeling G protein-coupled receptors; 19F-NMR; membrane mimetics; stable-isotope labeling; in-membrane chemical modification; amino-acid-specific NMR labeling; sequence-specific NMR labeling

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MDPI and ACS Style

Ge, H.; Wang, H.; Pan, B.; Feng, D.; Guo, C.; Yang, L.; Liu, D.; Wüthrich, K. G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling. Molecules 2022, 27, 2658. https://doi.org/10.3390/molecules27092658

AMA Style

Ge H, Wang H, Pan B, Feng D, Guo C, Yang L, Liu D, Wüthrich K. G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling. Molecules. 2022; 27(9):2658. https://doi.org/10.3390/molecules27092658

Chicago/Turabian Style

Ge, Haoyi, Huixia Wang, Benxun Pan, Dandan Feng, Canyong Guo, Lingyun Yang, Dongsheng Liu, and Kurt Wüthrich. 2022. "G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling" Molecules 27, no. 9: 2658. https://doi.org/10.3390/molecules27092658

APA Style

Ge, H., Wang, H., Pan, B., Feng, D., Guo, C., Yang, L., Liu, D., & Wüthrich, K. (2022). G Protein-coupled Receptor (GPCR) Reconstitution and Labeling for Solution Nuclear Magnetic Resonance (NMR) Studies of the Structural Basis of Transmembrane Signaling. Molecules, 27(9), 2658. https://doi.org/10.3390/molecules27092658

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