Extract from Black Soybean Cultivar A63 Extract Ameliorates Atopic Dermatitis-like Skin Inflammation in an Oxazolone-Induced Murine Model
Abstract
:1. Introduction
2. Results
2.1. Analysis of Isoflavones and Flavonoids in Korean Black Soybean Cultivars
2.2. A63 Extract Attenuates Oxazolone-Induced AD-like Skin Inflammation in Mice
2.3. The Application of A63 Rescued the Expression of Keratinocyte Differentiation Markers
2.4. A63 Extract Suppresses the Expression of AD Hallmark Genes in Mouse Tissue
2.5. MAPK and STAT Signaling Activation was Hindered by Treatment with A63 Extract
2.6. A63 Extract Prevents Eosinophil Infiltration toward the Inflammatory Site via CCL26 Expression Downregulation and the Inactivation of JAK1/STAT6 Signaling
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Analysis of Isoflavones and Other Flavonoids
4.3. Animal Study
4.4. Immunohistochemistry
4.5. RT-qPCR Analysis
4.6. Cell Culture
4.7. Western Blot Analysis
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Dorjsembe, B.; Nho, C.W.; Choi, Y.; Kim, J.-C. Extract from Black Soybean Cultivar A63 Extract Ameliorates Atopic Dermatitis-like Skin Inflammation in an Oxazolone-Induced Murine Model. Molecules 2022, 27, 2751. https://doi.org/10.3390/molecules27092751
Dorjsembe B, Nho CW, Choi Y, Kim J-C. Extract from Black Soybean Cultivar A63 Extract Ameliorates Atopic Dermatitis-like Skin Inflammation in an Oxazolone-Induced Murine Model. Molecules. 2022; 27(9):2751. https://doi.org/10.3390/molecules27092751
Chicago/Turabian StyleDorjsembe, Banzragch, Chu Won Nho, Yongsoo Choi, and Jin-Chul Kim. 2022. "Extract from Black Soybean Cultivar A63 Extract Ameliorates Atopic Dermatitis-like Skin Inflammation in an Oxazolone-Induced Murine Model" Molecules 27, no. 9: 2751. https://doi.org/10.3390/molecules27092751