5,2′-Dibromo-2,4′,5′-trihydroxydiphenylmethanone Inhibits LPS-Induced Vascular Inflammation by Targeting the Cav1 Protein
Abstract
:1. Introduction
2. Results
2.1. Effects of TDD on the Expression of TLR4 in LPS-Treated EA.hy926 Cells
2.2. Effects of TDD on the Expression of TLR4 in the Aortae of LPS-Treated Mice
2.3. Effects of TDD on Protein Expression
2.4. Validation of the Activity of the NF-κB Signaling
2.5. Effects of TDD on Selected Proteins
2.6. Effects of TDD on IL-6, IL-1β, MCP-1, and TNF-α Expression in LPS-Treated EA.hy926 Cells with Cav1 Knockdown
2.7. Effects of TDD on Inhibitor of NF-κB (IκB) and p65 Phosphorylation in LPS-Treated EA.hy926 Cells with Cav1 Knockdown
2.8. Biolayer Interference (BLI) Assay
2.9. UV-Vis Spectroscopy
2.10. Fluorescence Spectroscopy
2.11. Molecular Docking
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cell Culture
4.3. Animals and Treatments
4.4. Histopathological Evaluation
4.5. Enzyme-Linked Immunosorbent Assay (ELISA)
4.6. Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR)
4.7. Western Blot Analysis
4.8. Isobaric Tag for the Relative and Absolute Quantification Technique (iTRAQ)
4.9. Cav1 Adenovirus Infection
4.10. BLI Assay
4.11. UV-vis Absorption Spectra
4.12. Fluorescence Spectrum
4.13. Molecular Docking
4.14. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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T(K) | Ksv (L·mol−1) × 106 | Kq (L·mol−1·s−1) × 1014 | R2 |
---|---|---|---|
298 | 3.48 | 3.48 | 0.95 |
310 | 1.94 | 1.94 | 0.93 |
T(K) | Kb(L·mol−1) | n | R2 |
---|---|---|---|
298 | 1.09 × 109 | 1.3907 | 0.96 |
310 | 6.11 × 104 | 0.7436 | 0.96 |
Gene | Forward Primer Sequence (5′-3′) | Reverse Primer Sequence (5′-3′) |
---|---|---|
GAPDH (human) | TGGTGAAGACGCCAGTGGA | GCACCGTCAAGGCTGAGAAC |
TLR4 (human) | TAAGGTTGCCGCTTTCACTT | TGACCGAGCAGTTTCTGAGG |
MCP-1 (human) | TCAGCCAGATGCAATCAATG | AGATCTCCTTGGCCACAATG |
IL-6 (human) | GTGTGAAAGCAGCAAAGAG | CTCCAAAAGACCAGTGATG |
TNF-α (human) | CCTGTGAGGAGGACGAACAT | TTTGAGCCAGAAGAGGTTGAG |
IL-1β (human) | TGGCAGAAAGGGAACAGAAA | CTGGCTGATGGACAGGAGAT |
GAPDH (mouse) | AGGTCGGTGTGAACGGATTTG | TGTAGACCATGTAGTTGAGGTCA |
TLR4 (mouse) | GAGCCGGAAGGTTATTGTGGTAGTG | TCAAGGACAATGAAGATGATGCCAGAG |
MCP-1 (mouse) | CCACTCACCTGCTGCTACTCATTC | CTGCTGCTGGTGATCCTCTTGTAG |
IL-6 (mouse) | GATGGATGCTACCAAATGGA | CCAGGTAGCTATGGTACTCCAGAA |
TNF-α (mouse) | CCTCTAGCCCACGTCGTAGC | AGCAATGACTCCAAAGTAGACC |
IL-1β (mouse) | AGCAGCACATCAACAAGAGC | AGGTGCTCATGTCCTCATCC |
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Yuan, H.; Hou, Q.; Feng, X.; Zhang, Y.; Yang, F.; Ge, R.; Li, Q. 5,2′-Dibromo-2,4′,5′-trihydroxydiphenylmethanone Inhibits LPS-Induced Vascular Inflammation by Targeting the Cav1 Protein. Molecules 2022, 27, 2884. https://doi.org/10.3390/molecules27092884
Yuan H, Hou Q, Feng X, Zhang Y, Yang F, Ge R, Li Q. 5,2′-Dibromo-2,4′,5′-trihydroxydiphenylmethanone Inhibits LPS-Induced Vascular Inflammation by Targeting the Cav1 Protein. Molecules. 2022; 27(9):2884. https://doi.org/10.3390/molecules27092884
Chicago/Turabian StyleYuan, Hongxia, Qianyi Hou, Xiue Feng, Yuanlin Zhang, Fan Yang, Rui Ge, and Qingshan Li. 2022. "5,2′-Dibromo-2,4′,5′-trihydroxydiphenylmethanone Inhibits LPS-Induced Vascular Inflammation by Targeting the Cav1 Protein" Molecules 27, no. 9: 2884. https://doi.org/10.3390/molecules27092884