Next Article in Journal
Investigation of the Mechanism of Action of Periploca forrestii Schltr. Extract on Adjuvant Collagen Rats Based on UPLC-Q-Orbitrap-HRMS Non-Targeted Lipidomics
Next Article in Special Issue
Heme Spin Distribution in the Substrate-Free and Inhibited Novel CYP116B5hd: A Multifrequency Hyperfine Sublevel Correlation (HYSCORE) Study
Previous Article in Journal
Development and Evaluation of Topical Zinc Oxide Nanogels Formulation Using Dendrobium anosmum and Its Effect on Acne Vulgaris
Previous Article in Special Issue
Long-Term Characterization of Oxidation Processes in Graphitic Carbon Nitride Photocatalyst Materials via Electron Paramagnetic Resonance Spectroscopy
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 28
Issue 19
10.3390/molecules28196750
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Spin-Label Electron Paramagnetic Resonance Spectroscopy Reveals Effects of Wastewater Filter Membrane Coated with Titanium Dioxide Nanoparticles on Bovine Serum Albumin

by
Krisztina Sebők-Nagy
1,
Zoltán Kóta
1,
András Kincses
1,
Ákos Ferenc Fazekas
2,
András Dér
1,
Zsuzsanna László
2 and
Tibor Páli
1,*
1
Institute of Biophysics, Biological Research Centre Szeged, 6726 Szeged, Hungary
2
Department of Biosystems Engineering, Faculty of Engineering, University of Szeged, 6725 Szeged, Hungary
*
Author to whom correspondence should be addressed.
Molecules 2023, 28(19), 6750; https://doi.org/10.3390/molecules28196750
Submission received: 11 August 2023 / Revised: 19 September 2023 / Accepted: 20 September 2023 / Published: 22 September 2023
(This article belongs to the Special Issue Applied EPR Spectroscopy)
Browse Figures
Review Reports Versions Notes

Abstract

The accumulation of proteins in filter membranes limits the efficiency of filtering technologies for cleaning wastewater. Efforts are ongoing to coat commercial filters with different materials (such as titanium dioxide, TiO2) to reduce the fouling of the membrane. Beyond monitoring the desired effect of the retention of biomolecules, it is necessary to understand what the biophysical changes are in water-soluble proteins caused by their interaction with the new coated filter membranes, an aspect that has received little attention so far. Using spin-label electron paramagnetic resonance (EPR), aided with native fluorescence spectroscopy and dynamic light scattering (DLS), here, we report the changes in the structure and dynamics of bovine serum albumin (BSA) exposed to TiO2 (P25) nanoparticles or passing through commercial polyvinylidene fluoride (PVDF) membranes coated with the same nanoparticles. We have found that the filtering process and prolonged exposure to TiO2 nanoparticles had significant effects on different regions of BSA, and denaturation of the protein was not observed, neither with the TiO2 nanoparticles nor when passing through the TiO2-coated filter membranes.
Keywords: wastewater cleaning; polyvinylidene fluoride filter membrane; titanium dioxide nanoparticles; P25; serum albumin; spin-label EPR; fluorescence; dynamic light scattering wastewater cleaning; polyvinylidene fluoride filter membrane; titanium dioxide nanoparticles; P25; serum albumin; spin-label EPR; fluorescence; dynamic light scattering
Graphical Abstract

Share and Cite

MDPI and ACS Style

Sebők-Nagy, K.; Kóta, Z.; Kincses, A.; Fazekas, Á.F.; Dér, A.; László, Z.; Páli, T. Spin-Label Electron Paramagnetic Resonance Spectroscopy Reveals Effects of Wastewater Filter Membrane Coated with Titanium Dioxide Nanoparticles on Bovine Serum Albumin. Molecules 2023, 28, 6750. https://doi.org/10.3390/molecules28196750

AMA Style

Sebők-Nagy K, Kóta Z, Kincses A, Fazekas ÁF, Dér A, László Z, Páli T. Spin-Label Electron Paramagnetic Resonance Spectroscopy Reveals Effects of Wastewater Filter Membrane Coated with Titanium Dioxide Nanoparticles on Bovine Serum Albumin. Molecules. 2023; 28(19):6750. https://doi.org/10.3390/molecules28196750

Chicago/Turabian Style

Sebők-Nagy, Krisztina, Zoltán Kóta, András Kincses, Ákos Ferenc Fazekas, András Dér, Zsuzsanna László, and Tibor Páli. 2023. "Spin-Label Electron Paramagnetic Resonance Spectroscopy Reveals Effects of Wastewater Filter Membrane Coated with Titanium Dioxide Nanoparticles on Bovine Serum Albumin" Molecules 28, no. 19: 6750. https://doi.org/10.3390/molecules28196750

APA Style

Sebők-Nagy, K., Kóta, Z., Kincses, A., Fazekas, Á. F., Dér, A., László, Z., & Páli, T. (2023). Spin-Label Electron Paramagnetic Resonance Spectroscopy Reveals Effects of Wastewater Filter Membrane Coated with Titanium Dioxide Nanoparticles on Bovine Serum Albumin. Molecules, 28(19), 6750. https://doi.org/10.3390/molecules28196750

Article Metrics

Back to TopTop