Next Article in Journal
Crystal Structure and Intermolecular Energy for Some Nandrolone Esters
Previous Article in Journal
Steric and Energetic Studies on the Synergetic Enhancement Effect of Integrated Polyaniline on the Adsorption Properties of Toxic Basic and Acidic Dyes by Polyaniline/Zeolite-A Composite
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 28
Issue 20
10.3390/molecules28207178
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Current and Potential Applications of Monoterpenes and Their Derivatives in Oral Health Care

by
Wiktoria Potocka
1,2,*,
Zainab Assy
1,2,
Floris J. Bikker
1 and
Marja L. Laine
2
1
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands
2
Department of Periodontology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands
*
Author to whom correspondence should be addressed.
Molecules 2023, 28(20), 7178; https://doi.org/10.3390/molecules28207178
Submission received: 27 September 2023 / Revised: 13 October 2023 / Accepted: 16 October 2023 / Published: 19 October 2023
Browse Figure
Versions Notes

Abstract

:
Plant products have been employed in medicine for centuries. As the world becomes more health-conscious, there is a growing interest in natural and minimally processed products for oral health care. This has led to an increase in research into the bioactive compounds found in plant products, particularly monoterpenes. Monoterpenes are known to have beneficial biological properties, but the specific mechanisms by which they exert their effects are not yet fully understood. Despite this, some monoterpenes are already being used in oral health care. For example, thymol, which has antibacterial properties, is an ingredient in varnish used for caries prevention. In addition to this, monoterpenes have also demonstrated antifungal, antiviral, and anti-inflammatory properties, making them versatile for various applications. As research continues, there is potential for even more discoveries regarding the benefits of monoterpenes in oral health care. This narrative literature review gives an overview of the biological properties and current and potential applications of selected monoterpenes and their derivatives in oral health care. These compounds demonstrate promising potential for future medical development, and their applications in future research are expected to expand.

1. Introduction

Plants, as well as their derived compounds have been utilized in medicine and dentistry for centuries. For example, chew sticks, such as miswak, are thought to have been used in Babylonia as early as 3500 B.C. [1]. These sticks were made from twigs typically derived from aromatic trees such as the toothbrush tree (Salvadora persica) and applied as a toothbrush-like tool to mechanically remove (inter)dental debris. In addition, during brushing and chewing, the oils of the chew sticks were applied to the tooth surface and released into the saliva, potentially providing antimicrobial benefits and supporting oral health [2,3]. In turn, clove (Syzygium aromaticum) has been used as a natural anesthetic for treating toothache [4]. In addition to its analgesic properties, clove oil was found to possess anti-inflammatory and antioxidant properties and to aid in the wound-healing process [5,6]. In line with this, the practice of using clove oil to treat dental caries has been common since the sixteenth century, and in 1834, eugenol, which is the main active ingredient of clove oil, was extracted [4]. Eugenol was later established to belong to the class of terpenes [7].
Terpenes are a large and diverse group of naturally occurring compounds, generally found in plants. Over 30,000 terpenes have been described in the literature so far [8]. They are also termed isoprenoids, due to their structure comprising 2-methylbutane residues, often referred to as isoprene units, ((C5)n) [8]. Monoterpenes, belonging to a class of terpenes, consist of two linked isoprene units, which can be cyclized and oxidized in several ways. Monoterpenes and their derivatives can be found in plants like true lavender (Lavandula angustifolia) or basil (Ocimum basilicum). Depending on their structure, they are classified as acyclic, monocyclic, or bicyclic [7,9,10]. With over 1500 monoterpenes documented, their diverse biological and therapeutic properties make them promising candidates for use in medicine and dentistry [11].
The demand for natural and organic products has seen a steady rise, with the global market value for natural cosmetics and personal care items expected to grow by 40% between 2021 and 2027 [12,13]. This growing trend also extends to oral care products [14]. Consumers are showing a keen interest in products such as turmeric toothpaste [15,16] and oil-pulling blends enriched with volatile oils [17]. In line with this, interest in plant- and, in particular, monoterpene-based products is emerging, which often stems from the traditional use of home remedies that have been passed down through generations. Despite the recent surge in interest, many of these monoterpenes remain relatively unexplored in terms of their potential benefits and applications. Overall, the global market’s shift towards natural and organic options, particularly in the cosmetics, personal care, and oral care sectors, signifies a growing preference for products that are perceived as healthier and more environmentally friendly. This trend also presents exciting opportunities for the further exploration and development of novel monoterpene-based products.
In this review, the biological properties, practical uses, and applications of monoterpenes in the context of oral health care will be explored. These findings will provide an overview of the current literature and reveal the potential benefits of using monoterpenes in oral health care settings.

2. Biological Properties

The extraction of monoterpenes and their derivatives (monoterpenoids) from plant products has led to the discovery of their wide range of biological properties, some of which can be seen in Table 1. In this review, we classified these molecules based on their biological activity: antimicrobial and antiseptic, anti-cancer, anti-inflammatory, and analgesic and antinociceptive activities.

2.1. Antimicrobial and Antiseptic Activity

Numerous studies have demonstrated the antimicrobial potency of monoterpenes and monoterpenoids. Monoterpenoids are modified monoterpenes containing different functional groups such as alcohols, carboxylic acids, ketones, aldehydes, and phenols. Some of these compounds exhibit a broad-spectrum antimicrobial effect [96,97]. With an increasing prevalence of multi-drug-resistant pathogens, these compounds have drawn attention as alternative therapeutic agents. Taking into consideration that over 700 species of bacteria occupy the oral cavity [98], their eubiosis is imperative for the host’s oral health. Microbial dysbiosis has been involved in a broad range of oral diseases including caries, periodontal disease, and halitosis but also conditions such as odontogenic maxillary sinusitis, dentoalveolar abscesses, periradicular pathosis, jaw osteonecrosis, and jaw osteomyelitis [23]. Even systemic diseases, such as Alzheimer’s, cancer, cardiovascular disease, and diabetes, have been linked to oral microbial dysbiosis [99,100].

2.1.1. Antibacterial Activity

For centuries, plant-origin compounds have been employed in the treatment of oral bacterial infections [4,86,101]. For example, a screening of medicinal plants occurring on the Isle of Arran in Scotland, based on Meddygion Myddvai (a 14th-century Welsh manuscript used to treat conditions related to microbial infections), found that Juniper berries (Juniperus communis L.) exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli [86]. The antibacterial properties of this plant were attributed to the activity of sabinene, which is a natural bicyclic monoterpene found in juniper berries. To investigate the antibacterial properties of sabinene, a research group in Korea assessed the compound in vitro against the cariogenic Streptococcus mutans [89]. High concentrations of sabinene inhibited bacterial growth and biofilm formation and caused the downregulation of genes necessary for biofilm formation [89]. Another in vitro study investigated the synergistic effect of eugenol applied in combination with either ampicillin or gentamicin against the Streptococcus species S. mutans, Streptococcus sanguinis, Streptococcus sobrinus, Streptococcus ratti, Streptococcus criceti, Streptococcus anginosus, Streptococcus gordonii and the periodontitis-associated bacterial species Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Prevotella intermedia, and Porphyromonas gingivalis [55]. It was found that eugenol exhibited bactericidal and bacteriostatic activity. In like manner, hinokitiol (β-thujaplicin), a volatile oil component from a tropolone-derived monoterpenoid found naturally in cypress plants, inhibited the growth of oral bacteria, such as S. mutans, S. sobrinus, P. gingivalis, A. actinomycetemcomitans, F. nucleatum, and P. intermedia in vitro [64]. Furthermore, hinokitiol had a bactericidal effect against S. mutans, S. sobrinus, A. actinomycetemcomitans, F. nucleatum, and P. intermedia. Monoterpenoid thymol was also found to have antibacterial potential against S. mutans both in a single infection model and in co-infection with Candida albicans in vitro and in vivo [92].

2.1.2. Antifungal Activity

The prevalence of invasive fungal infections is estimated to be 1 billion annually, resulting in 1.5 million deaths worldwide [102,103]. The opportunistic pathogenic yeast C. albicans, responsible for causing candidiasis in the mouth, throat, and esophagus, has recently been included in the World Health Organization (WHO) fungal priority pathogen list due to its high antifungal resistance and the number of deaths caused [103]. Patients with dry mouth symptoms are particularly susceptible to oral candidiasis due to reduced salivary secretion [104]. Given the severity of the problem, novel therapies are being sought to control the infection spread and drug resistance.
The results of a screening of the antifungal properties of 16 monoterpenes against Candida species, including C. albicans, Candida dubliniensis, Candida glabrata, Candida guilliermondii, Candida krusei, and Candida tropicalis, revealed that (±)-limonene, (+)-α-pinene, and (±)-citronellol were the most effective inhibitors of fungal growth [24]. The inhibition of the growth of Candida auris was tested by combining phenolic monoterpenes with antifungal agents [95,105]. Carvacrol, found in oils from oregano (Origanum vulgare) and thyme (Thymus vulgaris), decreased the surface adherence levels and proteinase production of C. auris [105]. Furthermore, carvacrol exhibited either synergistic or additive activity when used together with antifungals such as fluconazole, amphotericin B, nystatin, and caspofungin. Thymol was also found to have anti-candidal activity. In vitro, thymol reduced the ability of Candida tropicalis and C. albicans to form biofilms, resulting in the disaggregation and deformation of fungal cells and decreased hyphae formation [95]. Furthermore, the presence of thymol reduced the integrity of pre-formed biofilms of both Candida species. In combination with the antifungal drug fluconazole, thymol had a synergistic effect on the biofilm and planktonic modes of growth of C. albicans and C. tropicalis. In turn, the monoterpene hinokitiol exhibited inhibitory activity against fluconazole-sensitive and fluconazole-resistant Candida species by impeding the fungal respiration and chelating the fungal intracellular iron in vitro [66]. As iron homeostasis is crucial for fungal survival, its perturbation is an efficient strategy for antifungal treatment [106]. Additionally, hinokitiol lowered the fungal burden in the Galleria mellonella larvae model infected with a C. albicans strain, compared to control and fluconazole treatment [66]. Further investigation revealed that hinokitiol chelated intracellular iron in C. albicans and inhibited mitochondrial respiration, by which the fungal growth was impeded.

2.1.3. Antiviral Activity

Viral infections of the oral mucosa are often present as blistering or ulceration. For example, herpes simplex virus type 1 (HSV-1) is a common infection transmitted through oral-to-oral contact, causing oral herpes in and around the mouth. Other viral infections that can cause blistering or ulceration of the oral mucosa include those caused by the varicella-zoster virus, coxsackievirus, and cytomegalovirus [107].
In a therapeutic screening of selected monoterpenes against HSV-1 in vitro, 1 h incubation of HSV-1 with monoterpenes resulted in 80–90% reduction of viral plaque when treated with α-terpinene, γ-terpinene, α-pinene, p-cymene, terpinen-4-ol, α-terpineol, thymol, citral, or eucalyptol (1,8-cineole) [26]. However, in an assay using the host African green monkey kidney RC-37 cell line treated with the above-mentioned monoterpenes prior to HSV-1 infection, only α-pinene caused a reduction in viral plaque by approximately 35%. α-Pinene likely killed the virus either by interfering with the virion envelope or by masking necessary viral structures [26]. In another experiment designed to impede viral replication cycles, only the presence of either α-pinene or eucalyptol reduced the viral plaque [26]. Further screenings against HSV-1 demonstrated that β-pinene and limonene had inhibitory potential against HSV-1 in RC-37 cells in vitro, possibly via interference with the virion envelope or by masking necessary viral structures [35]. On the other hand, isoborneol, a stereoisomer of borneol and eucalyptol, exhibited virucidal activity against HSV-1 in an in vitro assay. Isoborneol inhibited viral replication through the glycosylation of viral polypeptides of HSV-1 in Vero cells (African green monkey kidney epithelial cells) [69]. Furthermore, the Greek sage (Salvia fruticosa) components eucalyptol, camphor, and thujone (α- and β-thujone diastereomers mixture) exhibited viral inhibition when applied to HSV-1-infected Vero cells, with thujone having the highest activity [30]. Two monoterpenoids, perillyl alcohol and perillic acid, were also found to exhibit anti-HSV-1 potential by inhibiting the release of infective virion particles from Vero cells [82].
Another group of viruses that can cause oral ulcerations is that of the coxsackieviruses [107]. Some strains of coxsackievirus A and B can cause herpangina, characterized by aphthous-like ulcerations in the soft palate. Menthol was found to inhibit the coxsackievirus B3 in HeLa cells (epithelial carcinoma) and C57BL/6 mice [76]. Menthol showed antiviral effects by impairing mitochondrial fission in vitro and by decreasing the pancreatic viral titers measured by plaque assays in pancreatic homogenates in vivo. Furthermore, two enantiomers of limonene, R-(+)-limonene and S-(−)-limonene, showed antiviral potency against the coxsackievirus B3 and B4 in HEp-2 (epithelial carcinoma) cells [74].

2.2. Anti-Cancer Activity

Over 377,700 cases of lip and oral cavity cancers were reported in 2020 globally, making them the 16th most common malignancies [108]. In the United States in 2022, the five-year survival rate following the diagnosis of oral cavity or pharynx cancer was 67% [109]. Surgery and radiotherapy are the primary treatment modalities for oral cancers; however, these interventions are invasive and affect the daily functioning of the individual treated. Therefore, less-invasive pharmacological therapies are being explored.
In a study assessing the anti-cancer potential of compounds against oral cancer cell lines, hinokitiol was observed to be cytotoxic towards HS3, SAS, and SCC4 human tongue squamous carcinoma cell lines, while exhibiting lower cytotoxicity towards normal human oral keratinocytes [62]. On the other hand, (−)-β-pinene displayed comparable levels of cytotoxicity upon application to SCC9 and SCC25 human tongue squamous carcinoma cells and primary human fibroblasts [34]. Treatment with (−)-β-pinene resulted in increased cytotoxicity against cancerous cells when compared to dimethyl sulfoxide (DMSO) control treatment and treatments with caspase inhibitors. Furthermore, SCC9 cells had a significantly higher number of pyknotic nuclei than the control.
The effects of thymol on cell proliferation were evaluated in vitro using Cal27, SCC4, and SCC9 oral squamous cell carcinoma lines, where thymol was able to significantly decrease cell proliferation by mitochondria-mediated apoptosis [94]. On the other hand, an in vivo experiment in mice with Cal27 tumor xenografts demonstrated that thymol decreased tumor volume, including both cancerous as well as normal cells, with a significant reduction after 16 days. Additionally, thymol increased the number of apoptotic tumor cells when compared to the control. Another terpene, acyclic monoterpene geraniol, reduced the protein levels of molecules associated with tumors, such as p65 subunit nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB-p65) in the nucleus, tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS/NOS2) in a Wistar rat model of 4-nitroquinoline-1-oxide (4NQO)-induced tongue carcinogenesis when compared to the control [59]. As the upregulation of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway was associated with tumor promotion, the ability of geraniol to decrease the NF-κB-p65 levels in the nucleus would suggest its potential anti-tumor capacity.

2.3. Anti-Inflammatory Activity

Anti-inflammatory drugs are essential for mitigating inflammation and oxidative stress in tissues. Nonsteroidal anti-inflammatory drugs are frequently utilized in dentistry for pain relief; however, they can induce some negative side effects, including gastrointestinal problems, kidney disease, headaches, dizziness, and cardiovascular problems [110]. Because of the potential side effects, new compounds are being studied to substitute the commonly employed anti-inflammatory drugs.
Sage (Salvia officinalis L.) extracts have been traditionally employed as home remedies for the relief of inflammation and discomfort associated with gingivitis [31]. Evidence suggests that sage possesses analgesic and anti-inflammatory properties. Sage is rich in monoterpenes such as eucalyptol, borneol, camphor, and α-/β-thujone. The in vitro application of these monoterpenes to human gingival fibroblasts reduced interleukin-6 (IL-6) receptor and interleukin-8 (IL-8) receptor release. Elevated levels of IL-6 and IL-8 are often associated with periodontitis [111,112,113]. α-Terpineol has a characteristic smell reminiscent of lilac and can be isolated from pine and bitter orange oils, as well as lime fruits, in small quantities. An in vitro study using a carcinoma buccal (KB) cell line reported a reduction in the intracellular production of IL-6 in KB cells treated with α-terpineol when compared to the control [29]. Furthermore, it was found to decrease the gene expression of the IL-6 receptor.

2.4. Analgesic and Antinociceptive Activity

The term orofacial pain encompasses any pain experienced in the mouth, jaws, or face and is frequently caused by dental conditions. Despite recent advancements in research, the management of dental-related pain remains challenging and can significantly impact an individual’s quality of life [114]. The effective treatment of orofacial pain requires a comprehensive understanding of the underlying causes and an accurate diagnosis.
An in vivo study evaluated the analgesic effects of a β-cyclodextrin-containing carvacrol complex (CARV-βCD) in a Swiss mouse model of induced orofacial nociception [43]. Nociception is often assessed by measuring either the number of rubbings or the time spent by the animals rubbing the injected area with their fore or hind paws [115]. It is assessed over the period of two phases, the first phase lasting most commonly from 0 to 5 min, and the second phase from 15 to 40 min after the injection. The research found that CARV-βCD significantly reduced the number of nociceptive behaviors in Swiss mice with formalin-induced nociception, whereas carvacrol alone did not [43]. Conversely, in cases of capsaicin- and glutamate-induced orofacial nociception, both CARV-βCD and carvacrol significantly decreased the number of face rubbings. The mode of action of the CARV-βCD complex is thought to be via opioid, vanilloid, or glutamate systems [43]. Another study assessed the ability of citronellal to alleviate pain induced by formaldehyde, capsaicin, and glutamate in Swiss mice [47]. Citronellal was found to reduce the number of face rubbings due to pain caused by all three agents, which are thought to either inhibit substance P release or directly block action of neurokinin-1 (NK-1) receptor [47]. In vivo research in Wister rats assessed the effects of the administration of α-pinene alone and in combination with either bicuculline or naloxone on capsaicin-induced dental pulp nociception. It was found that treatment with α-pinene alone decreased the cumulative time of nociceptive behaviors. The mechanism of modulation may to be related to interactions with γ-aminobutyric acid type A (GABAA) receptors [23]. On the other hand, myrtenol was only able to decrease the face rubbing time in the second phase of the formalin test [79]. Myrtenol is thought to reduce orofacial nociception and inflammation through cytokine inhibition and the p38 mitogen-activated protein kinase (p38-MAPK) signal transduction pathway in trigeminal ganglia. Myrtenol also decreased IL-1β and myeloperoxidase (MPO) activity, both being inflammatory indicators.

3. Use in Dentistry and Oral Health Care

Natural products, including monoterpenes, can be found in both professional and over-the-counter consumer products within all fields of oral health care (Figure 1). In this review, their applications were classified based on their utilization, such as oral hygiene, halitosis, dental treatments, periodontal disease, mucosal lesions, and dry mouth.

3.1. Oral Hygiene

Monoterpenes, monoterpenoids, and volatile oils are commonly used as flavorings and perfuming agents in oral health care products. To exemplify, menthol, citral, limonene, hinokitiol, and eugenol, among others, can often be found in toothpaste, mouthwashes, and oral gels. Although there are insufficient appropriate data to substantiate the health benefits of herbal components in oral health care products [116], monoterpenes are commonly added to everyday care products in the form of volatile oils. It is worth noting that many manufacturers do not report the specific compounds present in oral care products. For example, toothpaste can contain mint oil; yet, menthol, the main ingredient, would not be reported. Despite that, it is safe to assume that monoterpenes and monoterpenoids can be found in a wide range of oral care products as fragrances and flavorings.
Toothpaste frequently contains menthol or volatile oils rich in menthol, acting as flavors and fragrances. Other monoterpenes and monoterpenoids such as carvone (0.00005–0.35%) [117] and hinokitiol (0.01–0.20%) [118] can also be found in toothpaste ingredients acting as antibacterial agents. On the other hand, limonene, linalool, citral, geraniol, and citronellol are employed as fragrance ingredients [14]. Toothpaste tablets, which are an alternative to traditional toothpaste, contain low concentrations of monoterpenes and monoterpenoids including menthol, linalool, citral, citronellol, limonene, and geraniol [119,120]. Both toothpaste and toothpaste tablets often contain volatile oils like southern blue gum leaf (Eucalyptus globulus), peppermint (Mentha × piperita), and lemon (Citrus limon), which are abundant in monoterpenes and their derivatives, i.e., respectively, eucalyptol, menthol, and limonene [14]. Menthol and “fresh”-smelling monoterpenes may also be infused into the bristles of toothbrushes and interdental brushes.
Mouthwashes are a staple product line in oral care, with Listerine (McNeil Consumer Healthcare, United States) being one of the most well-known brands. It contains monoterpenes and monoterpenoids such as eucalyptol, menthol, and thymol [121]. Other brands also use carvone, citral, citronellol, eugenol, geraniol, limonene, and linalool [122]. Additionally, monoterpene- and monoterpenoid-rich volatile oils are used, including clove (S. aromaticum), rosemary (Salvia rosmarinus), common sage (S. officinalis), Spanish sage (Salvia lavandulaefolia), and common thyme (T. vulgaris).
Molecules 28 07178 g001
Figure 1. Application of selected monoterpenes and monoterpenoids in oral health care and dentistry. Chemical structures were obtained from PubChem [18], and images from PNGegg [123].
Figure 1. Application of selected monoterpenes and monoterpenoids in oral health care and dentistry. Chemical structures were obtained from PubChem [18], and images from PNGegg [123].
Molecules 28 07178 g001

3.2. Halitosis

Intra-oral halitosis, bad breath, is often a symptom of bad oral hygiene and/or health issues within the oral cavity and can negatively impact an individual’s social functioning and quality of life. It is estimated that around 30% of the population suffers from halitosis, ranging from mild to severe [124]. Treatment involves addressing the underlying causes, including oral hygiene, periodontal diseases, endodontic infections, and dry mouth [125].
The efficacy of an antibacterial hinokitiol-containing gel on the severity of oral malodor was assessed in patients with diagnosed intra-oral halitosis using an organoleptic test (OLT) and gas chromatography [118]. The treatment group using hinokitiol had significantly lower OLT scores than the control group. This finding was confirmed using gas chromatography; the levels of hydrogen sulfide and methyl mercaptan, which are compounds associated with intra-oral halitosis, were decreased after the hinokitiol treatment. Additionally, in the same study, the use of hinokitiol reduced the number of sites with bleeding on probing (BOP), the average probing pocket depth, and the plaque index after 28 days. Thymoquinone, a monoterpenic quinone found in black cumin oil, was shown to have deodorizing activity and to be able to reduce the smell of methyl mercaptan by 80% [126].
Citrus-like scents, such as those found in citronellal, limonene, or citral, were identified as potential candidates for masking malodor [127]. For this purpose, odor sensor mice were trained to distinguish the smell of dimethyl sulfide, a volatile sulfur compound involved in bad breath. The mice were unable to distinguish the smell of dimethyl sulfide when citronellal, limonene, or citral was applied to the malodorous sample. The potency of lemon oil, which contains limonene as its main constituent [71], was evaluated in saliva from patients with bad breath [128]. The lemon volatile oil was found to reduce the levels of volatile sulfur compounds and bacterial growth and inhibit biofilm formation. Finally, though a lemongrass oil mouth rinse was found to significantly decrease volatile sulfur compounds over time, it did not exhibit antimicrobial potential against halitosis-associated microorganisms despite containing high levels of the monoterpenoids α-citral, β-citral, and geraniol [129].

3.3. Dental Treatments

Caries is a widespread condition affecting most of the worldwide population. Over 2 billion people are affected by untreated dental caries in permanent dentition, making it the most prevalent condition in the world [130]. Severe untreated cases of early childhood caries (ECC) and the consequent inflammatory reactions from pulp infections are contributing factors to stunting and underweight in children [130].
The monoterpenoid thymol is currently being considered as a potential therapeutic for the management of ECC [92] due to its antibacterial properties [92,93]. In an in vitro analysis, thymol inhibited the growth of and killed the cariogenic S. mutans and opportunistic C. albicans. It is thought that there might be an association between ECC and S. mutans and C. albicans colonization [131,132,133,134]. In this study, thymol interrupted biofilm formation in the presence of human saliva, suppressed the transition of C. albicans cells to a hyphal form, and decreased the acid production in S. mutans [92]. Furthermore, genes associated with the virulence of these pathogens were downregulated. The application of a chlorhexidine–thymol-containing varnish in carious lesions was also analyzed in children [135,136,137]. Both thymol and chlorhexidine have antimicrobial effects [138]. A chlorhexidine–thymol varnish, Cervitec (Ivoclar, Liechtenstein), was more effective than a fluoride varnish in decreasing the levels of S. mutans [135,137]. However, due to high standard deviations, there was no significant difference in the occurrence of caries between the test and the control groups [135,136,137]. Comparable studies were carried out in elderly patients [139,140]. The application of the chlorhexidine–thymol varnish decreased the size of the lesions and increased the distance of the lesions from the gingival margin when compared to the fluoride varnish, thus decreasing the severity of root caries [139]. Furthermore, a study of the geriatric population found that the chlorhexidine–thymol varnish reduced the size of root caries lesions and decreased the occurrence of new root caries [140]. In an animal model, dental caries were induced in rats by S. sobrinus infection [141]. The treatment of gingivae and teeth with a limonene solution was found to reduce the colony-forming units (CFUs) of both total bacteria and S. sobrinus compared to the control. Additionally, the number of caries lesions on the smooth surfaces of rat molars was significantly lower in the limonene treatment group. Another in vitro study researched the impact of limonene on ion release, surface microhardness recovery, and hydroxyproline concentration using enamel slabs [142]. Limonene did not express a better action potential than sodium fluoride in preventing demineralization and promoting remineralization.
In dental pulp treatments, hinokitiol can be applied as an endodontic material [143,144]. This monoterpenoid expresses antibacterial properties against Enterococcus faecalis [143] and S. aureus [144]. Hinokitiol-treated human dental pulp cells had significantly lower COX-2 and interleukin-1 (IL-1) concentrations when compared to the control [143]. Furthermore, odontoblastic differentiation markers (alkaline phosphatase (ALP), dentin matrix protein 1 (DMP-1), and dentin sialophosphoprotein (DSP)) and calcium mineral deposits were positively affected by the presence of hinokitiol. The addition of hinokitiol together with tricalcium silicate-based materials to the calcium phosphate cement (CPC) resulted in reduced working and setting times and increased compressive strength compared to the treatment with CPC alone [144]. There was no improvement in these qualities when CPC was supplemented with hinokitiol only. The addition of hinokitiol significantly increased the radiopacity of the cement samples.

3.4. Periodontal Diseases

Severe forms of periodontitis are estimated to affect 19% of the global adult population [130], while less severe gingivitis is thought to be prevalent in 29–88% of the population depending on location, age, and health status [145,146,147]. Periodontitis can result in tooth loss if left untreated. The current treatments involve improving oral hygiene, decreasing bacterial load, and removing calculus by subgingival debridement.
Carvacrol showed various ameliorative effects in rat models [148,149,150]. For example, the use of carvacrol reduced alveolar bone loss in the Wistar rat model compared to the control [148]. The use of carvacrol applied together with chalcones [149], which are naturally occurring polyphenolic compounds that belong to the flavonoids family [151], significantly decreased alveolar bone loss. However, this effect was not observed after treatment with carvacrol alone. Additionally, it was found that both carvacrol alone [148] and carvacrol in combination with chalcones [149] reduced the levels of MPO activity and the number of periodontitis-associated bacteria. When tested in Sprague–Dawley rats with ligation-induced periodontitis, carvacrol treatment decreased alveolar bone loss and the relative mRNA expression of inflammatory indicators such as Tnfa, Il1b, Il6, and Nos2 [150]. On the other hand, hinokitiol was demonstrated to have anti-inflammatory properties in the BALB/c mouse model [61]. Ligature-induced experimental periodontitis was produced in the animals, and periodontal bone loss was measured in the presence of hinokitiol. The application of hinokitiol resulted in decreased periodontal bone loss and inhibited oral bacterial growth. Furthermore, the relative mRNA transcription in murine gingival tissue of Il6, Il1b, Tnf, and NLR family pyrin domain-containing 3 (Nlrp3), which are associated with pro-inflammatory cytokines, was decreased. Comparable findings were observed within the murine RAW264.7 macrophage cell line, where treatment with hinokitiol decreased the relative mRNA transcription of Il6, Il1b, and Tnf, but not of Nlrp3 in vitro [61]. A decrease in the levels of pro-inflammatory cytokines would suggest the anti-inflammatory properties of hinokitiol.
In human randomized controlled trial, antimicrobial thymol- and menthol-containing gels were applied as a treatment for gingivitis in orthodontic patients [152]. The patients in the experimental group used the gels between the first (T1) and the second (T2) orthodontic treatment visits and stopped their usage between the second (T2) and the third (T3) visits. The BOP, probing depth, and gingival index (GI) scores decreased in the treatment group while using the gel compared to the placebo control group. Furthermore, a significant difference was observed between the T1–T2 and the T2–T3 time periods for BOP and GI.
Hydrogels can serve as biodegradable scaffolds for periodontal therapy. A thymol-chitosan hydrogel was designed as an alternative periodontal treatment to improve drug delivery and reduce the dosing intervals [153]. An in vitro assessment of the scaffold demonstrated that the application of thymol to the chitosan hydrogel resulted in lower viability and no aggregation of S. mutans inside the scaffold when compared to the application of chitosan hydrogel blanks. Microsponges can offer another way of antimicrobial drug delivery during periodontal treatment [154,155]. Eugenyl methacrylate microsponges loaded with eugenol decreased GI and tooth mobility in vivo [154]. Comparable results were observed for thymol microsponges [155].

3.5. Mucosal Lesions

Mucosal lesions, commonly referred to as mouth ulcers, can result from various etiologies including oral Candida, HSV infections, and cancer. These lesions often cause pain and discomfort and are treated with corticosteroids and antimicrobials [156]. However, due to the side effects associated with these drug classes, less invasive approaches are desired.
To address this, a study was conducted utilizing chemotherapy-induced mucositis in a Wister rat model to determine the efficacy of an oral gel containing borneol [157]. Borneol can be extracted from plants such as wild carrot (Daucus carota), common thyme (T. vulgaris), common sage (S. officinalis), and rosemary (S. rosmarinus) and is thought to have anti-inflammatory properties [31,37]. Results demonstrated that in animals treated with a 2.4% borneol oral gel, the cicatrization process improved at the seven-day time point when compared to the control [157]. Additionally, increased collagen levels and decreased inflammatory cell counts were observed. Rats treated with 1.2% and 2.4% borneol lost less weight during a period of 14 days than the control due to improved wound healing. Comparable research was carried out in a Sprague–Dawley rat model using a common guava (Psidium guajava L.) extract [158]. Guava flower and leaf extracts are abundant in α-pinene, β-pinene, and limonene. Rats with experimentally induced oral buccal mucosa wounds were exposed to a common guava leaf extract mouthwash, which resulted in a decrease in IL-6 levels in male rats at days 7 and 10, but not at day 14 when compared to the group with induced wounds and no intervention. The same relationship was only observed in female rats on day 14. No significant differences were observed between the control group and the guava treatment groups, except for female rats at day 10. Furthermore, histopathological changes were significantly more severe in the wounded animals’ intervention group when compared to the guava treatment group.

3.6. Dry Mouth

A dry mouth can be diagnosed subjectively and objectively; xerostomia is a sensation of having oral dryness, while hyposalivation is characterized by absolute reduced salivation. These symptoms can occur either separately or together, as hyposalivation-induced xerostomia. Treatment involves the use of either products stimulating the salivary flow, or, when the salivary glands have completely lost their function, saliva substitutes such as gels, sprays, or mouthwashes [159,160]. However, these substitutes often have a short-term effect and unpleasant texture and taste, resulting in many patients discontinuing their use [161]. One potential solution is the use of novel therapies based on monoterpenes and their derivatives, which offer a broad selection of flavors and fragrances due to their extensive profiles [162]. Sjögren’s disease patients affected by xerostomia indicated their preference for neutral and menthol-flavored saliva substitutes [163].
Ginger (Zingiber officinale) is known for possessing medicinal properties due to its chemical composition rich in terpenes and terpenoids, including monoterpenes and monoterpenoids such as (−)-β-phellandrene, geraniol, geranial (trans-citral), eucalyptol, and citral [164,165,166]. To exemplify, C57BL/6 mice were treated with ginger rhizome extracts dissolved in either ethanol or DMSO, and pilocarpine-stimulated salivary flow was measured [167]. The salivary flow rate was found to increase in the intervention groups when compared to their baselines. Furthermore, the ginger rhizome extracts significantly enhanced the salivary flow rate in the intervention groups when compared to the control [167]. In human participants, a study assessed the effectiveness of ginger infusion on salivary secretion in smokers with reduced salivary flow [168]. In this experiment, saliva secretion was determined by sialometry. Ginger infusion was found to increase the unstimulated and stimulated salivary volume after 28 days. In another study, ginger was applied as a saliva stimulant in a form of an oral spray for type II diabetes mellitus [169]. The results using a Schirmer test modified for salivary flow rate revealed an increase in salivary flow after treatment with the ginger spray. The patients also reported a decreased subjective feeling of dry mouth.
Mallow (Malva sylvestris) and hollyhock (Alcea digitata (Boiss)) are members of the plant family Malvaceae. The volatile oil of M. sylvestris is rich in monoterpenes and monoterpenoids, with a high percentage of eugenol [170], eucalyptol, α-terpinyl acetate, and menthol [171]. Mallow and hollyhock have both been used in traditional Persian medicine for treating dry mouth [172,173]. A study using the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire, Head and Neck Module (EORTC QLQ-H&N 35), found that head and neck cancer patients reported easier swallowing after using a combination drug of mallow and hollyhock compared to an artificial saliva substitute [173].
Mouthwashes are commonly used in individuals with xerostomia to relieve the feeling of a dry mouth. Licorice (Glycyrrhizae) spp. root is abundant in bioactive ingredients, including monoterpenes and their derivatives, such as α-caryophyllene, β-caryophyllene, myrcene, and α-pinene [174]. A study investigated the effects of a licorice mouthwash on salivary flow and xerostomia in hemodialysis patient [175]. It was found that the use of the licorice mouthwash resulted in an increase in unstimulated salivary flow rate compared to the control group. Additionally, the group using the licorice mouthwash reported a decreased feeling of dry mouth compared to both the control group and the group using a water-based mouthwash.
The impact of grapefruit oil, which is abundant in limonene [176], was evaluated on salivary secretion in a Sprague–Dawley rat model [177]. However, there was no significant difference in salivary flow between rats stimulated with grapefruit oil and the control group [177]. In human participants, the benefits of inhaling kuromoji (Lindera umbellata) oil and bergamot oil on the salivary function were analyzed [178]. The main volatile components of kuromoji volatile oil are linalool, eucalyptol, and D-limonene [179], while the main constituents of bergamot oil are limonene and linalool [180]. The bergamot volatile oil was found to increase the salivary flow both immediately and 25 min after inhalation, while the kuromoji oil resulted in increased salivation 25 min after inhalation, but not immediately. An organ scanning technology, scintigraphy, was used to measure salivary gland function in individuals following radioactive iodine therapy for thyroid cancer [181]. D-limonene and β-pinene were the primary monoterpene constituents in the applied lemon and ginger (2:1) oil. Aromatherapy with the volatile oil resulted in a significant increase in the accumulation ratio in the parotid and submandibular glands. Furthermore, there was a significant rise in the rate of secretion change before and after the treatment in the bilateral parotid glands. In another study, healthy volunteers were stimulated ortho-nasally with either mastic, which is a resin rich in α-pinene, or pure α-pinene in the form of volatile oil sticks [accepted] [182]. The stimulation with mastic and α-pinene resulted in increased salivary flow when compared to unstimulated participants. Mastic, but not α-pinene, significantly decreased spinnbarkeit, which is a measurement describing the rheology of saliva. These findings suggest that exposure to the mastic odor increased the production of serous saliva. Another human participant study analyzed the impact of a menthol mouth rinse on the salivary flow [183]. Menthol increased the salivary flow and total protein concentration in the treated volunteers compared to the unstimulated participants. Its mode of action is thought to be via an agonistic interaction with the transient receptor potential cation channel subfamily M (melastatin) member 8 channel (TRPM8).

4. Side Effects

4.1. Allergic Reactions

Monoterpenes are being investigated for their potential beneficial biomedical effects and applications. However, it is important to consider the potential for negative side effects. Eugenol, which used to be commonly employed as a root canal sealer in dentistry, was found to cause hypersensitivity, urticaria, and gingivitis, as well as allergic reactions [184,185,186,187,188,189]. Some reports indicated that eugenol allergy symptoms can resemble burning mouth syndrome [190,191]. Additionally, while uncommon, some toothpaste ingredients [192], including menthol [193] and carvone [194,195,196,197], can cause dermatological issues including contact dermatitis. In line with this, a retrospective study analyzed carvone patch tests results. Of the 4221 individuals tested, 3.48% (N = 147) were positive for carvone sensitization. Within the carvone-positive group, 73% of the participants reported oral signs, and 57% had oral lichens [198].

4.2. Toxicity

Monoterpenes and monoterpenoids possess various beneficial properties for the user and generally can be found in professional commodities and over-the-counter products and are used as home remedies. Many of these compounds have been approved for safe application by organs including the European Chemicals Agency (ECHA) [199] and designated as generally recognized as safe (GRAS) [200]. ECHA and the GRAS designation provide guidance and safe concentration ranges for different applications. For example, the maximum acceptable final concentration in products ranges between 0.25 and 7.30% for eugenol [201], 0.017 and 2.30% for citronellal [46], and 0.16 and 7.30% for α-pinene [202]. However, it is important to note that like any other substance, they can become toxic in relatively high concentrations (Table 2).
In a comparative in vitro study examining the impact of selected monoterpenes on human cells, the cell toxicity of camphor, eucalyptol, and thujone was assessed [203]. All three compounds were cytotoxic to MRC-5 fetal lung fibroblasts, with half maximal inhibitory concentration (IC50) values of 11 mM for camphor, 11 mM for eucalyptol, and 2.2 mM for thujone. Another form of toxicity, genotoxicity, describes the ability of substances to damage the genetic material in the cell. Camphor, eucalyptol, and thujone showed a genotoxic effect in MRC-5 and Vero cells. However, at concentrations up to 50 μM, the monoterpenes were found to induce a protective response in Vero cells against UV-induced mutagenesis and 4NQO-induced DNA strand breaks. In a comparable study, the results from trypan blue and MTT cytotoxicity assays revealed that citral had cytotoxic and genotoxic effects on peripheral blood mononuclear cells (PBMC) and the human hepatoma cell line HepG2 [205]. Citral was found to be genotoxic to both PBMC and HepG2 cells at a concentration above 50 μg/mL [205], while a camphor derivative, camphorquinone, was found to be genotoxic in the OKF6/TERT-2 telomerase-immortalized cell line above the concentration of 2.5 mM [206].
Various endodontics-focused research articles looked at the composition of root canal sealers. Zinc oxide eugenol root canal sealers were analyzed for their cytotoxicity in human periodontal ligament cells (PDLCs) and a permanent hamster V79 cell line [210]. Eugenol-containing root canal sealers were found to decrease the viability of both PDLCs and V79 cells. Comparable results were reported in human normal oral cells, primary gingival fibroblasts, PDLCs, and hemopoietic progenitor cells [204]. Eugenol was cytotoxic towards these cells with a 50% cytotoxic concentration (CC50) above 755 μM.
Injury to the liver tissue can be an outcome of chemical-driven monoterpene toxicity. Some monoterpenes have been proven or are thought to have potential hepatotoxicity in humans in high concentrations, including compounds such as citral, eugenol, camphor, D-limonene, geranial, menthofuran, neral, and pulegone [211,212]. For example, an in vivo analysis found pulegone to be hepatotoxic to rats at relatively high concentrations (400 mg/kg) [207], while limonene was harmful at 25 and 75 mg/kg [208].
Some monoterpenes can also cause neurotoxicity. For example, thujone, which occurs naturally in sage, rosemary, and thyme volatile oils, is used as a common food flavoring and is a constituent of the alcoholic drink absinthe. However, above certain levels, it can have poisonous effects and cause neurotoxicity. A mixture of α- and β-thujone had a median lethal dose 50 (LD50) of 192 mg/kg in rats, 230 mg/kg in mice, and 396 mg/kg in guinea pigs [213,214,215]. Another potentially neurotoxic monoterpene, α-terpinene, caused DNA damage, memory deficit, and had overall neurotoxic effects when applied orally in Wistar rats [209].

5. Materials and Methods

The review of the literature was performed using the databases of PubMed, Web of Science, Scopus, and Google Scholar until August 2023. The databases were searched for articles using keywords related to (monoterpene), (monoterpenes), (monoterpenoid), (monoterpenoids), (volatile compound), (volatile oil) AND (antimicrobial), (antiseptic), (antibacterial), (antiviral), (antifungal), (anti-cancer), (anti-inflammatory), (analgesic), (anti-nociceptive), (oral hygiene), (oral health), (oral), (mouth), (halitosis), (dentistry), (dental), (dental treatment), (periodontal diseases), (periodontitis), (gingivitis), (mucosal lesions), (dry mouth), (xerostomia), (saliva), (salivary flow), (salivation), (hyposalivation), (side effects), (allergy), (allergic reaction), (toxicity), (toxic). Further manual search was carried out to enroll other potentially relevant articles, which could not be found with the electronic search. Two authors (W.P. and Z.A.) independently searched for articles and examined the title and abstract of all records identified. The authors assessed each of these articles to determine which met the inclusion criteria for this review. For all articles that initially met the inclusion criteria, the full text was read. The inclusion criteria used for the present study were:
  • Availability of the full text;
  • Appropriate methodology;
  • Research regarding pure monoterpenes or monoterpenoids or mixed compounds containing monoterpenes or monoterpenoids.

6. Conclusions and Prospects

Monoterpenes and their derivatives are a unique and diverse class of compounds that have only partially been explored. As presented above, they possess significant biological properties, ranging from antimicrobial and anti-inflammatory effects to anticancer activities. However, the true potential of these compounds may lie in their ability to work in combination with modern treatments, potentially enhancing their efficacy and reducing their side effects. Hence, given their potential health benefits for humans, monoterpenes and monoterpenoids represent a promising source for the development of novel compounds that could be applied in general health care, but also specifically in oral health. For example, monoterpenes could be used as natural and effective alternatives to traditional antimicrobial agents. The discovery of molecular targets will lead to a better understanding of the mechanisms of action of the monoterpenes and monoterpenoids of interest and has the potential to support the discovery of new agents showing an improved therapeutic effect and decreased toxic properties. Furthermore, the unique properties of monoterpenes, such as their wide availability, natural origin, and being safe to use, make them attractive candidates for developing new drug (delivery) systems, which could improve the bioavailability and effectiveness of existing therapeutic agents.
However, as monoterpenes have only recently gained attention as potential therapeutic agents in modern health care, there are gaps in the literature that need to be addressed. These include a general lack of knowledge about the optimal dosages, routes of administration, and potential side effects of these compounds. Some of the literature cited in this review suffers from study design flaws, highlighting the need for more rigorous and well-designed studies in the future. New research plans need to be devised to gain a better understanding of how monoterpenes can be utilized in the field of oral health and beyond.

Author Contributions

Conceptualization, W.P., Z.A., F.J.B. and M.L.L.; methodology, W.P. and Z.A.; validation, W.P., Z.A., F.J.B. and M.L.L.; formal analysis, W.P. and Z.A.; investigation, W.P. and Z.A.; resources, F.J.B. and M.L.L.; data curation, W.P. and Z.A.; writing—original draft preparation, W.P.; writing—review and editing, W.P., Z.A., F.J.B. and M.L.L.; visualization, W.P.; supervision, F.J.B. and M.L.L.; project administration, F.J.B. and M.L.L. funding acquisition, F.J.B. and M.L.L. All authors have read and agreed to the published version of the manuscript.

Funding

This research was partly funded by the Nederlandse Organisatie voor Wetenschappelijk Onderzoek/Netherlands Organization for Applied Scientific research (NWO) grant number 19217. The APC was funded by the NWO.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

During the preparation of this work, the authors used Notion AI to improve readability and language. After using this tool/service, the authors reviewed and edited the content as needed and take full responsibility for the content of the publication.

Conflicts of Interest

The authors declare no conflict of interest.

Sample Availability

Not applicable.

References

  1. Zhou, Z.-R.; Yu, H.-Y.; Zheng, J.; Qian, L.-M.; Yan, Y. Dental Biotribology; Springer: New York, NY, USA, 2013; ISBN 978-1-4614-4549-4. [Google Scholar]
  2. Alali, F.; Hudaib, M.; Aburjai, T.; Khairallah, K.; Al-Hadidi, N. GC-MS Analysis and Antimicrobial Activity of the Essential Oil from the Stem of the Jordanian Toothbrush Tree Salvadora persica. Pharm. Biol. 2005, 42, 577–580. [Google Scholar] [CrossRef]
  3. Farag, M.; Abdel-Mageed, W.M.; El Gamal, A.A.; Basudan, O.A. Salvadora persica L.: Toothbrush Tree with Health Benefits and Industrial Applications—An Updated Evidence-Based Review. Saudi Pharm. J. 2021, 29, 751–763. [Google Scholar] [CrossRef] [PubMed]
  4. Medico-Dental History of Cloves. Nature 1943, 151, 194. [CrossRef]
  5. Alqareer, A.; Alyahya, A.; Andersson, L. The Effect of Clove and Benzocaine versus Placebo as Topical Anesthetics. J. Dent. 2006, 34, 747–750. [Google Scholar] [CrossRef]
  6. Jirovetz, L.; Buchbauer, G.; Stoilova, I.; Stoyanova, A.; Krastanov, A.; Schmidt, E. Chemical Composition and Antioxidant Properties of Clove Leaf Essential Oil. J. Agric. Food Chem. 2006, 54, 6303–6307. [Google Scholar] [CrossRef] [PubMed]
  7. Zielińska-Błajet, M.; Feder-Kubis, J. Monoterpenes and Their Derivatives—Recent Development in Biological and Medical Applications. Int. J. Mol. Sci. 2020, 21, 7078. [Google Scholar] [CrossRef] [PubMed]
  8. Breitmaier, E. Terpenes: Importance, General Structure, and Biosynthesis. In Terpenes; WILEY-VCH: Weinheim, Germany, 2006; pp. 1–9. ISBN 978-3-527-60994-9. [Google Scholar]
  9. Loza-Tavera, H. Monoterpenes in Essential Oils: Biosynthesis and Properties. Adv. Exp. Med. Biol. 1999, 464, 49–62. [Google Scholar] [CrossRef]
  10. Breitmaier, E. Hemi- and Monoterpenes. In Terpenes; WILEY-VCH: Weinheim, Germany, 2006; pp. 10–23. ISBN 978-3-527-60994-9. [Google Scholar]
  11. Alcántara, A.R.; Hernaiz, M.-J.; Sinisterra, J.-V. 3.28—Biocatalyzed Production of Fine Chemicals. In Comprehensive Biotechnology, 2nd ed.; Elsevier: Amsterdam, The Netherlands, 2011; pp. 309–331. ISBN 978-0-08-088504-9. [Google Scholar]
  12. Sinclair, L. Consumers Demand Natural Oral Hygiene Products. Available online: https://www.colgateprofessional.com/students-faculty/practice-management/consumers-demand-natural-oral-hygiene-products (accessed on 16 January 2023).
  13. Howarth, J. Top 9 Dental Industry Trends (2023–2025). Available online: https://explodingtopics.com/blog/dental-industry-trends (accessed on 20 January 2023).
  14. Mazur, M.; Ndokaj, A.; Bietolini, S.; Duś-Ilnicka, I.; Ottolenghi, L. Green Dentistry: Organic Toothpaste Formulations. A Literature Review. Dent. Med. Probl. 2022, 59, 461–474. [Google Scholar] [CrossRef]
  15. Colgate Naturals Turmeric Gum Protect Whitening Toothpaste. Available online: https://www.colgate.com/en-ph/products/toothpaste/colgate-naturals-turmeric-gum-protect (accessed on 25 January 2023).
  16. Turmeric for Your Teeth. Available online: https://www.colgate.com/en-us/oral-health/teeth-whitening/turmeric-for-teeth-will-a-spice-whiten-your-smile (accessed on 4 April 2023).
  17. Vedshakti Oil Pulling—Uses and Benefits. Available online: https://www.colgate.com/en-in/products/colgate-vedshakti-oil-pulling (accessed on 25 January 2023).
  18. PubChem PubChem. Available online: https://pubchem.ncbi.nlm.nih.gov/ (accessed on 9 May 2023).
  19. Allenspach, M.; Steuer, C. α-Pinene: A Never-Ending Story. Phytochemistry 2021, 190, 112857. [Google Scholar] [CrossRef]
  20. (+)-α-Pinene. Available online: https://www.wikidata.org/wiki/Q2095629 (accessed on 1 May 2023).
  21. (−)-α-Pinene. Available online: https://www.wikidata.org/wiki/Q300852 (accessed on 1 May 2023).
  22. α-Pinene. Available online: https://www.wikidata.org/wiki/Q27104380 (accessed on 1 May 2023).
  23. Rahbar, I.; Abbasnejad, M.; Haghani, J.; Raoof, M.; Kooshki, R.; Esmaeili-Mahani, S. The Effect of Central Administration of Alpha-Pinene on Capsaicin-Induced Dental Pulp Nociception. Int. Endod. J. 2019, 52, 307–317. [Google Scholar] [CrossRef]
  24. Iraji, A.; Yazdanpanah, S.; Alizadeh, F.; Mirzamohammadi, S.; Ghasemi, Y.; Pakshir, K.; Yang, Y.; Zomorodian, K. Screening the Antifungal Activities of Monoterpenes and Their Isomers against Candida Species. J. Appl. Microbiol. 2020, 129, 1541–1551. [Google Scholar] [CrossRef]
  25. Bomfim de Barros, D.; de Oliveira e Lima, L.; Alves da Silva, L.; Cavalcante Fonseca, M.; Ferreira, R.C.; Diniz Neto, H.; da Nóbrega Alves, D.; da Silva Rocha, W.P.; Scotti, L.; de Oliveira Lima, E.; et al. α-Pinene: Docking Study, Cytotoxicity, Mechanism of Action, and Anti-Biofilm Effect against Candida Albicans. Antibiotics 2023, 12, 480. [Google Scholar] [CrossRef] [PubMed]
  26. Astani, A.; Reichling, J.; Schnitzler, P. Comparative Study on the Antiviral Activity of Selected Monoterpenes Derived from Essential Oils. Phytother. Res. 2010, 24, 673–679. [Google Scholar] [CrossRef] [PubMed]
  27. Khaleel, C.; Tabanca, N.; Buchbauer, G. α-Terpineol, a Natural Monoterpene: A Review of Its Biological Properties. Open Chem. 2018, 16, 349–361. [Google Scholar] [CrossRef]
  28. α-Terpineol. Available online: https://www.wikidata.org/wiki/Q27109437 (accessed on 1 May 2023).
  29. Held, S.; Schieberle, P.; Somoza, V. Characterization of α-Terpineol as an Anti-Inflammatory Component of Orange Juice by in Vitro Studies Using Oral Buccal Cells. J. Agric. Food Chem. 2007, 55, 8040–8046. [Google Scholar] [CrossRef] [PubMed]
  30. Sivropoulou, A.; Nikolaou, C.; Papanikolaou, E.; Kokkini, S.; Lanaras, T.; Arsenakis, M. Antimicrobial, Cytotoxic, and Antiviral Activities of Salvia Fructicosa Essential Oil. J. Agric. Food Chem. 1997, 45, 3197–3201. [Google Scholar] [CrossRef]
  31. Ehrnhöfer-Ressler, M.M.; Fricke, K.; Pignitter, M.; Walker, J.M.; Walker, J.; Rychlik, M.; Somoza, V. Identification of 1,8-Cineole, Borneol, Camphor, and Thujone as Anti-Inflammatory Compounds in a Salvia officinalis L. Infusion Using Human Gingival Fibroblasts. J. Agric. Food Chem. 2013, 61, 3451–3459. [Google Scholar] [CrossRef]
  32. (−)-α-Thujone. Available online: https://www.wikidata.org/wiki/Q421838 (accessed on 1 May 2023).
  33. β-Pinene. Available online: https://www.wikidata.org/wiki/Q300928 (accessed on 1 May 2023).
  34. Machado, T.Q.; Felisberto, J.R.S.; Guimarães, E.F.; Queiroz, G.A.D.; Fonseca, A.C.C.D.; Ramos, Y.J.; Marques, A.M.; Moreira, D.d.L.; Robbs, B.K. Apoptotic Effect of β-Pinene on Oral Squamous Cell Carcinoma as One of the Major Compounds from Essential Oil of Medicinal Plant Piper Rivinoides Kunth. Nat. Prod. Res. 2022, 36, 1636–1640. [Google Scholar] [CrossRef]
  35. Astani, A.; Schnitzler, P. Antiviral Activity of Monoterpenes Beta-Pinene and Limonene against Herpes Simplex Virus In Vitro. Iran. J. Microbiol. 2014, 6, 149–155. [Google Scholar]
  36. β-Thujone. Available online: https://www.wikidata.org/wiki/Q27121861 (accessed on 2 May 2023).
  37. Borneol. Available online: https://www.wikidata.org/wiki/Q27103499 (accessed on 2 May 2023).
  38. Croteau, R.; Lee Hooper, C.; Felton, M. Biosynthesis of Monoterpenes: Partial Purification and Characterization of a Bicyclic Monoterpenol Dehydrogenase from Sage (Salvia officinalis). Arch. Biochem. Biophys. 1978, 188, 182–193. [Google Scholar] [CrossRef]
  39. Camphor. Available online: https://www.wikidata.org/wiki/Q181559 (accessed on 2 May 2023).
  40. Lee, S.-H.; Kim, D.-S.; Park, S.-H.; Park, H. Phytochemistry and Applications of Cinnamomum Camphora Essential Oils. Molecules 2022, 27, 2695. [Google Scholar] [CrossRef] [PubMed]
  41. Carvacrol. Available online: https://www.wikidata.org/wiki/Q225543 (accessed on 2 May 2023).
  42. De Vincenzi, M.; Stammati, A.; De Vincenzi, A.; Silano, M. Constituents of Aromatic Plants: Carvacrol. Fitoterapia 2004, 75, 801–804. [Google Scholar] [CrossRef] [PubMed]
  43. Silva, J.C.; Almeida, J.R.G.S.; Quintans, J.S.S.; Gopalsamy, R.G.; Shanmugam, S.; Serafini, M.R.; Oliveira, M.R.C.; Silva, B.A.F.; Martins, A.O.B.P.B.; Castro, F.F.; et al. Enhancement of Orofacial Antinociceptive Effect of Carvacrol, a Monoterpene Present in Oregano and Thyme Oils, by β-Cyclodextrin Inclusion Complex in Mice. Biomed. Pharmacother. 2016, 84, 454–461. [Google Scholar] [CrossRef] [PubMed]
  44. (RS)-Citronellal. Available online: https://www.wikidata.org/wiki/Q61651085 (accessed on 2 May 2023).
  45. Mahalwal, V.S.; Ali, M. Volatile Constituents of Cymbopogon nardus (Linn.) Rendle. Flavour Fragr. J. 2003, 18, 73–76. [Google Scholar] [CrossRef]
  46. Api, A.M.; Belsito, D.; Biserta, S.; Botelho, D.; Bruze, M.; Burton, G.A.; Buschmann, J.; Cancellieri, M.A.; Dagli, M.L.; Date, M.; et al. RIFM Fragrance Ingredient Safety Assessment, Citronellal, CAS Registry Number 106-23-0. Food Chem. Toxicol. 2021, 149, 111991. [Google Scholar] [CrossRef]
  47. Quintans-Júnior, L.J.; Melo, M.S.; De Sousa, D.P.; Araujo, A.A.S.; Onofre, A.C.S.; Gelain, D.P.; Gonçalves, J.C.R.; Araújo, D.A.M.; Almeida, J.R.G.S.; Bonjardim, L.R. Antinociceptive Effects of Citronellal in Formalin-, Capsaicin-, and Glutamate-Induced Orofacial Nociception in Rodents and Its Action on Nerve Excitability. J. Orofac. Pain 2010, 24, 305–312. [Google Scholar]
  48. (±)-β-Citronellol. Available online: https://www.wikidata.org/wiki/Q27122080 (accessed on 2 May 2023).
  49. Fekri, N.; El Amir, D.; Owis, A.; AbouZid, S. Studies on Essential Oil from Rose-Scented Geranium, Pelargonium graveolens L’Hérit. (Geraniaceae). Nat. Prod. Res. 2021, 35, 2593–2597. [Google Scholar] [CrossRef]
  50. Silva, D.; Diniz-Neto, H.; Cordeiro, L.; Silva-Neta, M.; Silva, S.; Andrade-Júnior, F.; Leite, M.; Nóbrega, J.; Morais, M.; Souza, J.; et al. (R)-(+)-β-Citronellol and (S)-(−)-β-Citronellol in Combination with Amphotericin B against Candida spp. Int. J. Mol. Sci. 2020, 21, 1785. [Google Scholar] [CrossRef]
  51. Sharma, Y.; Rastogi, S.K.; Perwez, A.; Rizvi, M.A.; Manzoor, N. β-Citronellol Alters Cell Surface Properties of Candida Albicans to Influence Pathogenicity Related Traits. Med. Mycol. 2020, 58, 93–106. [Google Scholar] [CrossRef]
  52. Eucalyptol. Available online: https://www.wikidata.org/wiki/Q161572 (accessed on 2 May 2023).
  53. Eugenol. Available online: https://www.wikidata.org/wiki/Q423357 (accessed on 2 May 2023).
  54. Mallavarapu, G.R.; Ramesh, S.; Chandrasekhara, R.S.; Rajeswara Rao, B.R.; Kaul, P.N.; Bhattacharya, A.K. Investigation of the Essential Oil of Cinnamon Leaf Grown at Bangalore and Hyderabad. Flavour Fragr. J. 1995, 10, 239–242. [Google Scholar] [CrossRef]
  55. Moon, S.-E.; Kim, H.-Y.; Cha, J.-D. Synergistic Effect between Clove Oil and Its Major Compounds and Antibiotics against Oral Bacteria. Arch. Oral Biol. 2011, 56, 907–916. [Google Scholar] [CrossRef] [PubMed]
  56. Ulanowska, M.; Olas, B. Biological Properties and Prospects for the Application of Eugenol—A Review. Int. J. Mol. Sci. 2021, 22, 3671. [Google Scholar] [CrossRef] [PubMed]
  57. Geraniol. Available online: https://www.wikidata.org/wiki/Q410836 (accessed on 2 May 2023).
  58. Martin, D.M.; Bohlmann, J. Identification of Vitis Vinifera (-)-Alpha-Terpineol Synthase by in Silico Screening of Full-Length cDNA ESTs and Functional Characterization of Recombinant Terpene Synthase. Phytochemistry 2004, 65, 1223–1229. [Google Scholar] [CrossRef] [PubMed]
  59. Madankumar, A.; Tamilarasi, S.; Premkumar, T.; Gopikrishnan, M.; Nagabhishek, N.; Devaki, T. Geraniol Attenuates 4NQO-Induced Tongue Carcinogenesis through Downregulating the Activation of NF-κB in Rats. Mol. Cell. Biochem. 2017, 434, 7–15. [Google Scholar] [CrossRef] [PubMed]
  60. β-Thujaplicin. Available online: https://www.wikidata.org/wiki/Q2705171 (accessed on 2 May 2023).
  61. Hiyoshi, T.; Domon, H.; Maekawa, T.; Yonezawa, D.; Kunitomo, E.; Tabeta, K.; Terao, Y. Protective Effect of Hinokitiol against Periodontal Bone Loss in Ligature-Induced Experimental Periodontitis in Mice. Arch. Oral Biol. 2020, 112, 104679. [Google Scholar] [CrossRef] [PubMed]
  62. Shih, Y.-H.; Chang, K.-W.; Hsia, S.-M.; Yu, C.-C.; Fuh, L.-J.; Chi, T.-Y.; Shieh, T.-M. In Vitro Antimicrobial and Anticancer Potential of Hinokitiol against Oral Pathogens and Oral Cancer Cell Lines. Microbiol. Res. 2013, 168, 254–262. [Google Scholar] [CrossRef]
  63. Lin, H.-C.; Wang, C.-C.; Wu, C.-F.; Lin, Y.-H.; Lee, W.-C.; Chen, P.-J.; Chang, Y.U.; Su, Y.-C. Hinokitiol Inhibits the Viability of Oral Squamous Carcinoma Cells by Inducing Apoptosis and Autophagy. Anticancer Res. 2023, 43, 1167–1173. [Google Scholar] [CrossRef]
  64. Domon, H.; Hiyoshi, T.; Maekawa, T.; Yonezawa, D.; Tamura, H.; Kawabata, S.; Yanagihara, K.; Kimura, O.; Kunitomo, E.; Terao, Y. Antibacterial Activity of Hinokitiol against Both Antibiotic-Resistant and -Susceptible Pathogenic Bacteria That Predominate in the Oral Cavity and Upper Airways. Microbiol. Immunol. 2019, 63, 213–222. [Google Scholar] [CrossRef]
  65. Morita, Y.; Sakagami, Y.; Okabe, T.; Ohe, T.; Inamori, Y.; Ishida, N. The Mechanism of the Bactericidal Activity of Hinokitiol. Biocontrol Sci. 2007, 12, 101–110. [Google Scholar] [CrossRef]
  66. Jin, X.; Zhang, M.; Lu, J.; Duan, X.; Chen, J.; Liu, Y.; Chang, W.; Lou, H. Hinokitiol Chelates Intracellular Iron to Retard Fungal Growth by Disturbing Mitochondrial Respiration. J. Adv. Res. 2021, 34, 65–77. [Google Scholar] [CrossRef]
  67. (+)-Isoborneol. Available online: https://www.wikidata.org/wiki/Q27270411 (accessed on 3 May 2023).
  68. (−)-Isoborneol. Available online: https://www.wikidata.org/wiki/Q15644616 (accessed on 3 May 2023).
  69. Armaka, M.; Papanikolaou, E.; Sivropoulou, A.; Arsenakis, M. Antiviral Properties of Isoborneol, a Potent Inhibitor of Herpes Simplex Virus Type 1. Antivir. Res. 1999, 43, 79–92. [Google Scholar] [CrossRef] [PubMed]
  70. (±)-Limonene. Available online: https://www.wikidata.org/wiki/Q278809 (accessed on 3 May 2023).
  71. Evans, W.C.; Evans, D. Volatile Oils and Resins. In Trease and Evans’ Pharmacognosy; Elsevier: Amsterdam, The Netherlands, 2009; pp. 263–303. ISBN 978-0-7020-2933-2. [Google Scholar]
  72. Thakre, A.; Zore, G.; Kodgire, S.; Kazi, R.; Mulange, S.; Patil, R.; Shelar, A.; Santhakumari, B.; Kulkarni, M.; Kharat, K.; et al. Limonene Inhibits Candida Albicans Growth by Inducing Apoptosis. Med. Mycol. 2018, 56, 565–578. [Google Scholar] [CrossRef] [PubMed]
  73. Ahmedi, S.; Pant, P.; Raj, N.; Manzoor, N. Limonene Inhibits Virulence Associated Traits in Candida Albicans: In-Vitro and in-Silico Studies. Phytomed. Plus 2022, 2, 100285. [Google Scholar] [CrossRef]
  74. Hamdi, A.; Halouani, A.; Aouf, I.; Viaene, J.; Marzouk, B.; Kraiem, J.; Jaïdane, H.; Heyden, Y.V. Cytotoxicity and Antiviral Activities of Haplophyllum Tuberculatum Essential Oils, Pure Compounds, and Their Combinations against Coxsackievirus B3 and B4. Planta Med. 2021, 87, 827–835. [Google Scholar] [CrossRef] [PubMed]
  75. L-Menthol. Available online: https://www.wikidata.org/wiki/Q407418 (accessed on 9 May 2023).
  76. Taylor, D.J.R.; Hamid, S.M.; Andres, A.M.; Saadaeijahromi, H.; Piplani, H.; Germano, J.F.; Song, Y.; Sawaged, S.; Feuer, R.; Pandol, S.J.; et al. Antiviral Effects of Menthol on Coxsackievirus B. Viruses 2020, 12, 373. [Google Scholar] [CrossRef]
  77. Myrtenol. Available online: https://www.wikidata.org/wiki/Q15425810 (accessed on 3 May 2023).
  78. Varlet, V.; Augsburger, M. Monitoring of Aglycons of Yew Glycosides (3,5-Dimethoxyphenol, Myrtenol and 1-Octen-3-Ol) as First Indicator of Yew Presence. Drug Test. Anal. 2013, 5, 474–479. [Google Scholar] [CrossRef]
  79. Oliveira, J.P.; Abreu, F.F.; Bispo, J.M.M.; Cerqueira, A.R.A.; Dos Santos, J.R.; Correa, C.B.; Costa, S.K.P.; Camargo, E.A. Myrtenol Reduces Orofacial Nociception and Inflammation in Mice Through P38-MAPK and Cytokine Inhibition. Front. Pharmacol. 2022, 13, 910219. [Google Scholar] [CrossRef]
  80. Perillic Acid. Available online: https://www.wikidata.org/wiki/Q27104388 (accessed on 9 May 2023).
  81. Wang, X.-F.; Li, H.; Jiang, K.; Wang, Q.-Q.; Zheng, Y.-H.; Tang, W.; Tan, C.-H. Anti-Inflammatory Constituents from Perilla frutescens on Lipopolysaccharide-Stimulated RAW264.7 Cells. Fitoterapia 2018, 130, 61–65. [Google Scholar] [CrossRef]
  82. Mello, C.P.; Quirico-Santos, T.; Amorim, L.F.; Silva, V.G.; Fragel, L.M.; Bloom, D.C.; Paixão, I.P. Perillyl Alcohol and Perillic Acid Exert Efficient Action upon HSV-1 Maturation and Release of Infective Virus. Antivir. Ther. 2020, 25, 1–11. [Google Scholar] [CrossRef]
  83. Opdyke, D.L.J. Monographs on Fragrance Raw Materials. Food Cosmet. Toxicol. 1981, 19, 237–254. [Google Scholar] [CrossRef]
  84. Karp, F.; Mihaliak, C.A.; Harris, J.L.; Croteau, R. Monoterpene Biosynthesis: Specificity of the Hydroxylations of (−)-Limonene by Enzyme Preparations from Peppermint (Mentha piperita), Spearmint (Mentha spicata), and Perilla (Perilla frutescens) Leaves. Arch. Biochem. Biophys. 1990, 276, 219–226. [Google Scholar] [CrossRef]
  85. Perillyl Alcohol. Available online: https://www.wikidata.org/wiki/Q15391928 (accessed on 9 May 2023).
  86. Wagner, C.S.; De Gezelle, J.; Robertson, M.; Robertson, K.; Wilson, M.; Komarnytsky, S. Antibacterial Activity of Medicinal Plants from The Physicians of Myddvai, a 14th Century Welsh Medical Manuscript. J. Ethnopharmacol. 2017, 203, 171–181. [Google Scholar] [CrossRef] [PubMed]
  87. Dosoky, N.S.; Satyal, P.; Barata, L.M.; da Silva, J.K.R.; Setzer, W.N. Volatiles of Black Pepper Fruits (Piper nigrum L.). Molecules 2019, 24, 4244. [Google Scholar] [CrossRef] [PubMed]
  88. Sabinene. Available online: https://www.wikidata.org/wiki/Q421278 (accessed on 3 May 2023).
  89. Park, B.-I.; Kim, B.-S.; Kim, K.-J.; You, Y.-O. Sabinene Suppresses Growth, Biofilm Formation, and Adhesion of Streptococcus Mutans by Inhibiting Cariogenic Virulence Factors. J. Oral Microbiol. 2019, 11, 1632101. [Google Scholar] [CrossRef]
  90. Thymol. Available online: https://www.wikidata.org/wiki/Q408883 (accessed on 3 May 2023).
  91. Salehi, B.; Mishra, A.P.; Shukla, I.; Sharifi-Rad, M.; Contreras, M.D.M.; Segura-Carretero, A.; Fathi, H.; Nasrabadi, N.N.; Kobarfard, F.; Sharifi-Rad, J. Thymol, Thyme, and Other Plant Sources: Health and Potential Uses. Phytother. Res. 2018, 32, 1688–1706. [Google Scholar] [CrossRef] [PubMed]
  92. Priya, A.; Selvaraj, A.; Divya, D.; Karthik Raja, R.; Pandian, S.K. In Vitro and In Vivo Anti-Infective Potential of Thymol Against Early Childhood Caries Causing Dual Species Candida Albicans and Streptococcus Mutans. Front. Pharmacol. 2021, 12, 760768. [Google Scholar] [CrossRef]
  93. Khan, S.T.; Khan, M.; Ahmad, J.; Wahab, R.; Abd-Elkader, O.H.; Musarrat, J.; Alkhathlan, H.Z.; Al-Kedhairy, A.A. Thymol and Carvacrol Induce Autolysis, Stress, Growth Inhibition and Reduce the Biofilm Formation by Streptococcus Mutans. AMB Express 2017, 7, 49. [Google Scholar] [CrossRef]
  94. De La Chapa, J.J.; Singha, P.K.; Lee, D.R.; Gonzales, C.B. Thymol Inhibits Oral Squamous Cell Carcinoma Growth via Mitochondria-Mediated Apoptosis. J. Oral Pathol. Med. Off. Publ. Int. Assoc. Oral Pathol. Am. Acad. Oral Pathol. 2018, 47, 674–682. [Google Scholar] [CrossRef]
  95. Jafri, H.; Ahmad, I. Thymus Vulgaris Essential Oil and Thymol Inhibit Biofilms and Interact Synergistically with Antifungal Drugs against Drug Resistant Strains of Candida Albicans and Candida Tropicalis. J. Mycol. Médicale 2020, 30, 100911. [Google Scholar] [CrossRef]
  96. Kotan, R.; Kordali, S.; Cakir, A. Screening of Antibacterial Activities of Twenty-One Oxygenated Monoterpenes. Z. Für Naturforschung C 2007, 62, 507–513. [Google Scholar] [CrossRef]
  97. Pattnaik, S.; Subramanyam, V.R.; Bapaji, M.; Kole, C.R. Antibacterial and Antifungal Activity of Aromatic Constituents of Essential Oils. Microbios 1997, 89, 39–46. [Google Scholar] [PubMed]
  98. Kilian, M.; Chapple, I.L.C.; Hannig, M.; Marsh, P.D.; Meuric, V.; Pedersen, A.M.L.; Tonetti, M.S.; Wade, W.G.; Zaura, E. The Oral Microbiome—An Update for Oral Healthcare Professionals. Br. Dent. J. 2016, 221, 657–666. [Google Scholar] [CrossRef] [PubMed]
  99. Al-Manei, K.; Ghorbani, M.; Naud, S.; Al-Manei, K.K.; Sobkowiak, M.J.; Lund, B.; Hazirolan, G.; Sällberg Chen, M.; Özenci, V. Clinical Microbial Identification of Severe Oral Infections by MALDI-TOF Mass Spectrometry in Stockholm County: An 11-Year (2010 to 2020) Epidemiological Investigation. Microbiol. Spectr. 2022, 10, e02487-22. [Google Scholar] [CrossRef]
  100. de Jongh, C.A.; de Vries, T.J.; Bikker, F.J.; Gibbs, S.; Krom, B.P. Mechanisms of Porphyromonas Gingivalis to Translocate over the Oral Mucosa and Other Tissue Barriers. J. Oral Microbiol. 2023, 15, 2205291. [Google Scholar] [CrossRef]
  101. Bifulco, M.; Amato, M.; Gangemi, G.; Marasco, M.; Caggiano, M.; Amato, A.; Pisanti, S. Dental Care and Dentistry Practice in the Medieval Medical School of Salerno. Br. Dent. J. 2016, 221, 87–89. [Google Scholar] [CrossRef] [PubMed]
  102. Bongomin, F.; Gago, S.; Oladele, R.O.; Denning, D.W. Global and Multi-National Prevalence of Fungal Diseases—Estimate Precision. J. Fungi 2017, 3, 57. [Google Scholar] [CrossRef]
  103. WHO. Fungal Priority Pathogens List to Guide Research, Development and Public Health Action; World Health Organization: Geneva, Switzerland, 2022.
  104. Jensena, J.L.; Barkvoll, P. Clinical Implications of the Dry Mouth: Oral Mucosal Diseases. Ann. N. Y. Acad. Sci. 1998, 842, 156–162. [Google Scholar] [CrossRef]
  105. Shaban, S.; Patel, M.; Ahmad, A. Improved Efficacy of Antifungal Drugs in Combination with Monoterpene Phenols against Candida Auris. Sci. Rep. 2020, 10, 1162. [Google Scholar] [CrossRef]
  106. Fourie, R.; Kuloyo, O.O.; Mochochoko, B.M.; Albertyn, J.; Pohl, C.H. Iron at the Centre of Candida Albicans Interactions. Front. Cell. Infect. Microbiol. 2018, 8, 185. [Google Scholar] [CrossRef]
  107. Neville, B.W.; Damm, D.D.; Allen, C.M.; Chi, A.C. Chapter 7 Viral Infections. In Oral and Maxillofacial Pathology; Elsevier Health Sciences: Amsterdam, The Netherlands, 2015; ISBN 978-1-4557-7052-6. [Google Scholar]
  108. Mouth and Oral Cancer Statistics. Available online: https://www.wcrf.org/cancer-trends/mouth-and-oral-cancer-statistics/ (accessed on 14 February 2023).
  109. Cancer Facts & Figures 2022; American Cancer Society: Atlanta, GA, USA, 2022.
  110. NSAIDs. Available online: https://www.nhs.uk/conditions/nsaids/ (accessed on 15 February 2023).
  111. Finoti, L.S.; Nepomuceno, R.; Pigossi, S.C.; Corbi, S.C.; Secolin, R.; Scarel-Caminaga, R.M. Association between Interleukin-8 Levels and Chronic Periodontal Disease: A PRISMA-Compliant Systematic Review and Meta-Analysis. Medicine 2017, 96, e6932. [Google Scholar] [CrossRef]
  112. Caldeira, F.I.D.; Hidalgo, M.A.R.; De Carli Dias, M.L.; Scarel-Caminaga, R.M.; Pigossi, S.C. Systematic Review of Ratios between Disease /Health Periodontitis Modulators and Meta-Analysis of Their Levels in Gingival Tissue and Biological Fluids. Arch. Oral Biol. 2021, 127, 105147. [Google Scholar] [CrossRef] [PubMed]
  113. Plemmenos, G.; Evangeliou, E.; Polizogopoulos, N.; Chalazias, A.; Deligianni, M.; Piperi, C. Central Regulatory Role of Cytokines in Periodontitis and Targeting Options. Curr. Med. Chem. 2021, 28, 3032–3058. [Google Scholar] [CrossRef]
  114. Pau, A.; Croucher, R.; Marcenes, W.; Leung, T. Development and Validation of a Dental Pain-Screening Questionnaire. Pain 2005, 119, 75–81. [Google Scholar] [CrossRef]
  115. Luccarini, P.; Childeric, A.; Gaydier, A.-M.; Voisin, D.; Dallel, R. The Orofacial Formalin Test in the Mouse: A Behavioral Model for Studying Physiology and Modulation of Trigeminal Nociception. J. Pain 2006, 7, 908–914. [Google Scholar] [CrossRef] [PubMed]
  116. Janakiram, C.; Venkitachalam, R.; Fontelo, P.; Iafolla, T.J.; Dye, B.A. Effectiveness of Herbal Oral Care Products in Reducing Dental Plaque & Gingivitis—A Systematic Review and Meta-Analysis. BMC Complement. Med. Ther. 2020, 20, 43. [Google Scholar] [CrossRef]
  117. Kroona, L.; Warfvinge, G.; Isaksson, M.; Ahlgren, C.; Dahlin, J.; Sörensen, Ö.; Bruze, M. Quantification of L-Carvone in Toothpastes Available on the Swedish Market. Contact Dermat. 2017, 77, 224–230. [Google Scholar] [CrossRef]
  118. Iha, K.; Suzuki, N.; Yoneda, M.; Takeshita, T.; Hirofuji, T. Effect of Mouth Cleaning with Hinokitiol-Containing Gel on Oral Malodor: A Randomized, Open-Label Pilot Study. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. 2013, 116, 433–439. [Google Scholar] [CrossRef]
  119. Smyle Toothpaste Tabs Ingredients. Available online: https://wesmyle.com/pages/ingredients (accessed on 5 April 2023).
  120. Refresher Toothpaste Tabs. Available online: https://www.lushusa.com/face/oral-care/refresher/9999961260.html (accessed on 2 March 2023).
  121. Adams, D.; Addy, M. Mouthrinses. Adv. Dent. Res. 1994, 8, 291–301. [Google Scholar] [CrossRef]
  122. Radzki, D.; Wilhelm-Węglarz, M.; Pruska, K.; Kusiak, A.; Ordyniec-Kwaśnica, I. A Fresh Look at Mouthwashes-What Is Inside and What Is It for? Int. J. Environ. Res. Public Health 2022, 19, 3926. [Google Scholar] [CrossRef]
  123. PNGEgg—Image Hosting. Available online: https://www.pngegg.com/ (accessed on 7 August 2023).
  124. Silva, M.F.; Leite, F.R.M.; Ferreira, L.B.; Pola, N.M.; Scannapieco, F.A.; Demarco, F.F.; Nascimento, G.G. Estimated Prevalence of Halitosis: A Systematic Review and Meta-Regression Analysis. Clin. Oral Investig. 2018, 22, 47–55. [Google Scholar] [CrossRef]
  125. Kapoor, U.; Sharma, G.; Juneja, M.; Nagpal, A. Halitosis: Current Concepts on Etiology, Diagnosis and Management. Eur. J. Dent. 2016, 10, 292–300. [Google Scholar] [CrossRef] [PubMed]
  126. Nakasugi, T.; Murakawa, T.; Shibuya, K.; Morimoto, M. Deodorizing Substance in Black Cumin (Nigella sativa L.) Seed Oil. J. Oleo Sci. 2017, 66, 877–882. [Google Scholar] [CrossRef]
  127. Osada, K.; Hanawa, M.; Tsunoda, K.; Izumi, H. Evaluation of the Masking of Dimethyl Sulfide Odors by Citronellal, Limonene and Citral through the Use of Trained Odor Sensor Mice. Chem. Senses 2013, 38, 57–65. [Google Scholar] [CrossRef]
  128. Ma, L.; Pang, C.; Yan, C.; Chen, J.; Wang, X.; Hui, J.; Zhou, L.; Zhang, X. Effect of Lemon Essential Oil on Halitosis. Oral Dis. 2022, 29, 1845–1854. [Google Scholar] [CrossRef] [PubMed]
  129. Satthanakul, P.; Taweechaisupapong, S.; Paphangkorakit, J.; Pesee, M.; Timabut, P.; Khunkitti, W. Antimicrobial Effect of Lemongrass Oil against Oral Malodour Micro-Organisms and the Pilot Study of Safety and Efficacy of Lemongrass Mouthrinse on Oral Malodour. J. Appl. Microbiol. 2015, 118, 11–17. [Google Scholar] [CrossRef] [PubMed]
  130. Global Oral Health Status Report: Towards Universal Health Coverage for Oral Health by 2030; World Health Organization: Geneva, Switzerland, 2022.
  131. Xiao, J.; Huang, X.; Alkhers, N.; Alzamil, H.; Alzoubi, S.; Wu, T.T.; Castillo, D.A.; Campbell, F.; Davis, J.; Herzog, K.; et al. Candida Albicans and Early Childhood Caries: A Systematic Review and Meta-Analysis. Caries Res. 2018, 52, 102–112. [Google Scholar] [CrossRef]
  132. Parisotto, T.M.; Steiner-Oliveira, C.; Silva, C.M.; Rodrigues, L.K.; Nobre-dos-Santos, M. Early Childhood Caries and Mutans Streptococci: A Systematic Review. Oral Health Prev. Dent. 2010, 8, 59–70. [Google Scholar]
  133. Kirthiga, M.; Murugan, M.; Saikia, A.; Kirubakaran, R. Risk Factors for Early Childhood Caries: A Systematic Review and Meta-Analysis of Case Control and Cohort Studies. Pediatr. Dent. 2019, 41, 95–112. [Google Scholar]
  134. Orlova, E.; Dudding, T.; Chernus, J.M.; Alotaibi, R.N.; Haworth, S.; Crout, R.J.; Lee, M.K.; Mukhopadhyay, N.; Feingold, E.; Levy, S.M.; et al. Association of Early Childhood Caries with Bitter Taste Receptors: A Meta-Analysis of Genome-Wide Association Studies and Transcriptome-Wide Association Study. Genes 2022, 14, 59. [Google Scholar] [CrossRef]
  135. Flamee, S.; Gizani, S.; Caroni, C.; Papagiannoulis, L.; Twetman, S. Effect of a Chlorhexidine/Thymol and a Fluoride Varnish on Caries Development in Erupting Permanent Molars: A Comparative Study. Eur. Arch. Paediatr. Dent. 2015, 16, 449–454. [Google Scholar] [CrossRef]
  136. Splieth, C.; Steffen, H.; Rosin, M.; Welk, A. Caries Prevention with Chlorhexidine-Thymol Varnish in High Risk Schoolchildren. Community Dent. Oral Epidemiol. 2000, 28, 419–423. [Google Scholar] [CrossRef] [PubMed]
  137. Ben Khadra, G.M.; Arrag, E.A.; Alammori, M.; AlKadi, M.F. The Effect of Chlorhexidine-Thymol and Fluoride Varnishes on the Levels of Streptococcus Mutans in Saliva in Children Aged 6–8 Years. Indian J. Dent. Res. Off. Publ. Indian Soc. Dent. Res. 2019, 30, 67–72. [Google Scholar] [CrossRef]
  138. Marchese, A.; Orhan, I.E.; Daglia, M.; Barbieri, R.; Di Lorenzo, A.; Nabavi, S.F.; Gortzi, O.; Izadi, M.; Nabavi, S.M. Antibacterial and Antifungal Activities of Thymol: A Brief Review of the Literature. Food Chem. 2016, 210, 402–414. [Google Scholar] [CrossRef] [PubMed]
  139. Brailsford, S.R.; Fiske, J.; Gilbert, S.; Clark, D.; Beighton, D. The Effects of the Combination of Chlorhexidine/Thymol- and Fluoride-Containing Varnishes on the Severity of Root Caries Lesions in Frail Institutionalised Elderly People. J. Dent. 2002, 30, 319–324. [Google Scholar] [CrossRef]
  140. Baca, P.; Clavero, J.; Baca, A.P.; González-Rodríguez, M.P.; Bravo, M.; Valderrama, M.J. Effect of Chlorhexidine-Thymol Varnish on Root Caries in a Geriatric Population: A Randomized Double-Blind Clinical Trial. J. Dent. 2009, 37, 679–685. [Google Scholar] [CrossRef]
  141. Liu, Y.; Liu, P.; Wang, L.; Shi, Y.; Chen, J.; Wang, H.; Zhang, X. Inhibitory Effects of Citrus Lemon Oil and Limonene on Streptococcus Sobrinus—Induced Dental Caries in Rats. Arch. Oral Biol. 2020, 118, 104851. [Google Scholar] [CrossRef]
  142. Ma, L.; Chen, J.; Han, H.; Liu, P.; Wang, H.; Lin, S.; Zhang, Q.; Lu, D.; Zhang, X. Effects of Lemon Essential Oil and Limonene on the Progress of Early Caries: An In Vitro Study. Arch. Oral Biol. 2020, 111, 104638. [Google Scholar] [CrossRef]
  143. Huang, M.-H.; Shen, Y.-F.; Hsu, T.-T.; Huang, T.-H.; Shie, M.-Y. Physical Characteristics, Antimicrobial and Odontogenesis Potentials of Calcium Silicate Cement Containing Hinokitiol. Mater. Sci. Eng. C 2016, 65, 1–8. [Google Scholar] [CrossRef]
  144. Shieh, T.-M.; Hsu, S.-M.; Chang, K.-C.; Chen, W.-C.; Lin, D.-J. Calcium Phosphate Cement with Antimicrobial Properties and Radiopacity as an Endodontic Material. Materials 2017, 10, 1256. [Google Scholar] [CrossRef]
  145. Liu, X.; Xu, J.; Li, S.; Wang, X.; Liu, J.; Li, X. The Prevalence of Gingivitis and Related Risk Factors in Schoolchildren Aged 6-12 Years Old. BMC Oral Health 2022, 22, 623. [Google Scholar] [CrossRef]
  146. Erchick, D.J.; Rai, B.; Agrawal, N.K.; Khatry, S.K.; Katz, J.; LeClerq, S.C.; Reynolds, M.A.; Mullany, L.C. Oral Hygiene, Prevalence of Gingivitis, and Associated Risk Factors among Pregnant Women in Sarlahi District, Nepal. BMC Oral Health 2019, 19, 2. [Google Scholar] [CrossRef]
  147. Kassab, M.M.; Cohen, R.E. The Etiology and Prevalence of Gingival Recession. J. Am. Dent. Assoc. 2003, 134, 220–225. [Google Scholar] [CrossRef] [PubMed]
  148. Botelho, M.A.; Martins, J.G.; Ruela, R.S.; Rachid, I.; Santos, J.A.; Soares, J.B.; França, M.C.; Montenegro, D.; Ruela, W.S.; Barros, L.P.; et al. Protective Effect of Locally Applied Carvacrol Gel on Ligature-Induced Periodontitis in Rats: A Tapping Mode AFM Study. Phytother. Res. 2009, 23, 1439–1448. [Google Scholar] [CrossRef]
  149. Botelho, M.A.; Rao, V.S.; Montenegro, D.; Bandeira, M.A.M.; Fonseca, S.G.C.; Nogueira, N.A.P.; Ribeiro, R.A.; Brito, G.A.C. Effects of a Herbal Gel Containing Carvacrol and Chalcones on Alveolar Bone Resorption in Rats on Experimental Periodontitis. Phytother. Res. 2008, 22, 442–449. [Google Scholar] [CrossRef] [PubMed]
  150. Kuo, P.-J.; Hung, T.-F.; Lin, C.-Y.; Hsiao, H.-Y.; Fu, M.-W.; Hong, P.-D.; Chiu, H.-C.; Fu, E. Carvacrol Ameliorates Ligation-Induced Periodontitis in Rats. J. Periodontol. 2017, 88, e120–e128. [Google Scholar] [CrossRef]
  151. Zhuang, C.; Zhang, W.; Sheng, C.; Zhang, W.; Xing, C.; Miao, Z. Chalcone: A Privileged Structure in Medicinal Chemistry. Chem. Rev. 2017, 117, 7762–7810. [Google Scholar] [CrossRef] [PubMed]
  152. Martin, B.J.; Campbell, P.M.; Rees, T.D.; Buschang, P.H. A Randomized Controlled Trial Evaluating Antioxidant–Essential Oil Gel as a Treatment for Gingivitis in Orthodontic Patients. Angle Orthod. 2016, 86, 407–412. [Google Scholar] [CrossRef]
  153. Alvarez Echazú, M.I.; Olivetti, C.E.; Anesini, C.; Perez, C.J.; Alvarez, G.S.; Desimone, M.F. Development and Evaluation of Thymol-Chitosan Hydrogels with Antimicrobial-Antioxidant Activity for Oral Local Delivery. Mater. Sci. Eng. C 2017, 81, 588–596. [Google Scholar] [CrossRef]
  154. Patole, V.C.; Chaudhari, S.P. Eugenyl Methacrylate Microsponges Loaded with Eugenol Incorporated In Situ Gel for Treatment of Periodontitis. J. Pharm. Innov. 2021, 16, 408–418. [Google Scholar] [CrossRef]
  155. Patole, V.C.; Chaudhari, S.P. Development of Thymol Microsponges Loaded in Situ Gel for the Treatment of Periodontitis. Curr. Drug Deliv. 2021, 18, 71–87. [Google Scholar] [CrossRef]
  156. Weinberg, M.A.; Maloney, W.J. Treatment of Common Oral Lesions. Available online: https://www.uspharmacist.com/article/treatment-of-common-oral-lesions (accessed on 27 March 2023).
  157. do Nascimento-Júnior, B.J.; Brito, L.S.; Barros, W.N.; Gonçalves, D.M.; Matos, L.S.; Nascimento, C.R.B.; Ribeiro, L.A.A.; de Lima, R.S.; Silva-Júnior, R.G.C.; Reis, S.A.G.B.; et al. Anti-Inflammatory and Healing Action of Oral Gel Containing Borneol Monoterpene in Chemotherapy-Induced Mucositis in Rats (Rattus Norvegicus). Braz. J. Pharm. Sci. 2017, 53, e00081. [Google Scholar] [CrossRef]
  158. Ghaderi, F.; Ebrahimi, E.; Sari Aslani, F.; Koohi-Hosseinabadi, O.; Koohpeyma, F.; Irajie, C.; Tanideh, N.; Iraji, A. The Effect of Hydroalcoholic Extract of Psidium guajava L. on Experimentally Induced Oral Mucosal Wound in Rat. BMC Complement. Med. Ther. 2022, 22, 201. [Google Scholar] [CrossRef] [PubMed]
  159. Al Hamad, A.; Lodi, G.; Porter, S.; Fedele, S.; Mercadante, V. Interventions for Dry Mouth and Hyposalivation in Sjögren’s Syndrome: A Systematic Review and Meta-Analysis. Oral Dis. 2019, 25, 1027–1047. [Google Scholar] [CrossRef] [PubMed]
  160. Furness, S.; Worthington, H.V.; Bryan, G.; Birchenough, S.; McMillan, R. Interventions for the Management of Dry Mouth: Topical Therapies. Cochrane Database Syst. Rev. 2011, 12, CD008934. [Google Scholar] [CrossRef] [PubMed]
  161. Brand, H.S.; Ouzzine, R.; Bots, C.P. Sticky Saliva Products. Br. Dent. J. 2013, 214, 95. [Google Scholar] [CrossRef]
  162. Niklander, S.E.; Martínez, M.; Miranda, A.; Rodriguez, M. Treatment Alternatives for Dry Mouth: A Scoping Review. J. Clin. Exp. Dent. 2022, 14, e846–e853. [Google Scholar] [CrossRef]
  163. Assy, Z.; Bikker, F.J.; Mashhour, E.; Asadi, M.; Brand, H.S. Preferences of Sjögren’s Syndrome Patients Regarding Potential New Saliva Substitutes. Clin. Oral Investig. 2022, 26, 6245–6252. [Google Scholar] [CrossRef]
  164. Zhang, M.; Zhao, R.; Wang, D.; Wang, L.; Zhang, Q.; Wei, S.; Lu, F.; Peng, W.; Wu, C. Ginger Zingiber officinale and Its Bioactive Components Are Potential Resources for Health Beneficial Agents. Phytother. Res. 2021, 35, 711–742. [Google Scholar] [CrossRef]
  165. Wohlmuth, H.; Smith, M.K.; Brooks, L.O.; Myers, S.P.; Leach, D.N. Essential Oil Composition of Diploid and Tetraploid Clones of Ginger (Zingiber officinale Roscoe) Grown in Australia. J. Agric. Food Chem. 2006, 54, 1414–1419. [Google Scholar] [CrossRef]
  166. Gupta, S.; Pandotra, P.; Ram, G.; Anand, R.; Gupta, A.P.; Husain, M.K.; Bedi, Y.S.; Mallavarapu, G.R. Composition of a Monoterpenoid-Rich Essential Oil from the Rhizome of Zingiber officinale from North Western Himalayas. Nat. Prod. Commun. 2011, 6, 1934578X1100600122. [Google Scholar] [CrossRef]
  167. Kan, C.Y.; H’ng, J.X.; Goh, A.; Smales, F.; Tan, E.L.; Zhang, S.; Pichika, M.R.; Parolia, A. Effect of Sustained Systemic Administration of Ginger (Z officinale) Rhizome Extracts on Salivary Flow in Mice. Int. Dent. J. 2023, 73, 63–70. [Google Scholar] [CrossRef] [PubMed]
  168. Mesquita, M.; Silva, M.; Moncada, M.; Bernardo, M.; Silva, M.; Proença, L. Effect of a Ginger Infusion in Smokers with Reduced Salivary Flow Rate. Int. J. Clin. Res. Trials 2018, 3, 121. [Google Scholar] [CrossRef]
  169. Mardani, H.; Ghannadi, A.; Rashnavadi, B.; Kamali, R. The Effect of Ginger Herbal Spray on Reducing Xerostomia in Patients with Type II Diabetes. Avicenna J. Phytomed. 2017, 7, 308–316. [Google Scholar]
  170. Delfine, S.; Marrelli, M.; Conforti, F.; Formisano, C.; Rigano, D.; Menichini, F.; Senatore, F. Variation of Malva Sylvestris Essential Oil Yield, Chemical Composition and Biological Activity in Response to Different Environments across Southern Italy. Ind. Crops Prod. 2017, 98, 29–37. [Google Scholar] [CrossRef]
  171. Sharifi-Rad, J.; Melgar-Lalanne, G.; Hernández-Álvarez, A.J.; Taheri, Y.; Shaheen, S.; Kregiel, D.; Antolak, H.; Pawlikowska, E.; Brdar-Jokanović, M.; Rajkovic, J.; et al. Malva Species: Insights on Its Chemical Composition towards Pharmacological Applications. Phytother. Res. 2020, 34, 546–567. [Google Scholar] [CrossRef] [PubMed]
  172. Ameri, A.; Heydarirad, G.; Rezaeizadeh, H.; Choopani, R.; Ghobadi, A.; Gachkar, L. Evaluation of Efficacy of an Herbal Compound on Dry Mouth in Patients with Head and Neck Cancers: A Randomized Clinical Trial. J. Evid.-Based Complement. Altern. Med. 2016, 21, 30–33. [Google Scholar] [CrossRef] [PubMed]
  173. Heydarirad, G.; Rezaeizadeh, H.; Choopani, R.; Mosavat, S.H.; Ameri, A. Efficacy of a Traditional Persian Medicine Preparation for Radiation-Induced Xerostomia: A Randomized, Open-Label, Active-Controlled Trial. J. Integr. Med. 2017, 15, 201–208. [Google Scholar] [CrossRef]
  174. Firoznejhad, M.; Ghasemi Pirbalouti, A.; Abdoosi, V.; Aliahmadi, A. Chemical Composition of Essential Oils from the Underground Parts of Glycyrrhiza echinata L. Accessions Growing Wild in Northern Iran. Nat. Prod. Res. 2021, 35, 162–166. [Google Scholar] [CrossRef]
  175. Yu, I.-C.; Tsai, Y.-F.; Fang, J.-T.; Yeh, M.-M.; Fang, J.-Y.; Liu, C.-Y. Effects of Mouthwash Interventions on Xerostomia and Unstimulated Whole Saliva Flow Rate among Hemodialysis Patients: A Randomized Controlled Study. Int. J. Nurs. Stud. 2016, 63, 9–17. [Google Scholar] [CrossRef]
  176. Deng, W.; Liu, K.; Cao, S.; Sun, J.; Zhong, B.; Chun, J. Chemical Composition, Antimicrobial, Antioxidant, and Antiproliferative Properties of Grapefruit Essential Oil Prepared by Molecular Distillation. Molecules 2020, 25, 217. [Google Scholar] [CrossRef]
  177. Suda, K.; Takahashi, Y.; Matsuki-Fukushima, M.; Satoh, K. Effect of the Scent from Grapefruit Oil on Salivary Secretion in Rats. Int. J. Oral-Med. Sci. 2010, 9, 31–35. [Google Scholar] [CrossRef]
  178. Yamagami, A.; Imura, H.; Shibuya, K.; Murakawa, T.; Kono, Y.; Sugimoto, K. Effects of aroma on saliva secretion and activities of autonomic nerves and brain; Essential oil of kuromoji and bergamot. Jpn. J. Taste Smell Res. 2012, 19, 453–456. [Google Scholar]
  179. Nanashima, N.; Kitajima, M.; Takamagi, S.; Fujioka, M.; Tomisawa, T. Comparison of Chemical Composition between Kuromoji (Lindera Umbellata) Essential Oil and Hydrosol and Determination of the Deodorizing Effect. Molecules 2020, 25, 4195. [Google Scholar] [CrossRef] [PubMed]
  180. Sawamura, M.; Onishi, Y.; Ikemoto, J.; Tu, N.T.M.; Phi, N.T.L. Characteristic Odour Components of Bergamot (Citrus bergamia Risso) Essential Oil. Flavour Fragr. J. 2006, 21, 609–615. [Google Scholar] [CrossRef]
  181. Nakayama, M.; Okizaki, A.; Takahashi, K. A Randomized Controlled Trial for the Effectiveness of Aromatherapy in Decreasing Salivary Gland Damage Following Radioactive Iodine Therapy for Differentiated Thyroid Cancer. BioMed Res. Int. 2016, 2016, 9509810. [Google Scholar] [CrossRef]
  182. Faruque, M.R.J.; Nazmi, K.; van Splunter, A.; Laine, M.L.; Bikker, F.J. Sialogogic Effects through Olfactory Stimulation with Mastic Resin and α-Pinene Volatiles In Vivo. Biomed. Pharmacother. 2023; accepted. [Google Scholar]
  183. Houghton, J.W.; Carpenter, G.; Hans, J.; Pesaro, M.; Lynham, S.; Proctor, G. Agonists of Orally Expressed TRP Channels Stimulate Salivary Secretion and Modify the Salivary Proteome. Mol. Cell. Proteom. MCP 2020, 19, 1664–1676. [Google Scholar] [CrossRef]
  184. Sarrami, N.; Pemberton, M.N.; Thornhill, M.H.; Theaker, E.D. Adverse Reactions Associated with the Use of Eugenol in Dentistry. Br. Dent. J. 2002, 193, 257–259. [Google Scholar] [CrossRef]
  185. Barkin, M.E.; Boyd, J.P.; Cohen, S. Acute Allergic Reaction to Eugenol. Oral Surg. Oral Med. Oral Pathol. 1984, 57, 441–442. [Google Scholar] [CrossRef]
  186. Deshpande, A.; Verma, S.; Macwan, C. Allergic Reaction Associated with the Use of Eugenol Containing Dental Cement in a Young Child. Austin J. Dent. 2014, 1, 1007. [Google Scholar]
  187. Tammannavar, P.; Pushpalatha, C.; Jain, S.; SV, S. An Unexpected Positive Hypersensitive Reaction to Eugenol. BMJ Case Rep. 2013, 2013, bcr2013009464. [Google Scholar] [CrossRef] [PubMed]
  188. Silvestre, J.F.; Albares, M.P.; Blanes, M.; Pascual, J.C.; Pastor, N. Allergic Contact Gingivitis Due to Eugenol Present in a Restorative Dental Material. Contact Dermat. 2005, 52, 341. [Google Scholar] [CrossRef] [PubMed]
  189. Bhalla, M.; Thami, G.P. Acute Urticaria Due to Dental Eugenol. Allergy 2003, 58, 158. [Google Scholar] [CrossRef] [PubMed]
  190. Bui, T.N.P.T.; Mose, K.F.; Andersen, F. Eugenol Allergy Mimicking Burning Mouth Syndrome. Contact Dermat. 2019, 80, 54–55. [Google Scholar] [CrossRef] [PubMed]
  191. Navarro Triviño, F.J.; Cuenca-Barrales, C.; Ruiz-Villaverde, R. Eugenol Allergy Mimicking Recurrent Aphthous Stomatitis and Burning Mouth Syndrome. Contact Dermat. 2019, 81, 462–463. [Google Scholar] [CrossRef]
  192. de Groot, A. Contact Allergy to (Ingredients of) Toothpastes. Dermatitis 2017, 28, 95–114. [Google Scholar] [CrossRef]
  193. Bourgeois, P.; Goossens, A. Allergic Contact Cheilitis Caused by Menthol in Toothpaste and Throat Medication: A Case Report. Contact Dermat. 2016, 75, 113–115. [Google Scholar] [CrossRef]
  194. Worm, M.; Jeep, S.; Sterry, W.; Zuberbier, T. Perioral Contact Dermatitis Caused by L-Carvone in Toothpaste. Contact Dermat. 1998, 38, 338. [Google Scholar] [CrossRef]
  195. Hansson, C.; Bergendorff, O.; Wallengren, J. Contact Urticaria Caused by Carvone in Toothpaste. Contact Dermat. 2011, 65, 362–364. [Google Scholar] [CrossRef]
  196. Corazza, M.; Levratti, A.; Virgili, A. Allergic Contact Cheilitis Due to Carvone in Toothpastes. Contact Dermat. 2002, 46, 366–367. [Google Scholar] [CrossRef]
  197. Kroona, L.; Ahlgren, C.; Dahlin, J.; Isaksson, M.; Bruze, M. Use Test with L-Carvone in Toothpaste on Sensitized Individuals. Contact Dermat. 2023, 88, 463–471. [Google Scholar] [CrossRef] [PubMed]
  198. Kroona, L.; Isaksson, M.; Ahlgren, C.; Dahlin, J.; Bruze, M.; Warfvinge, G. Carvone Contact Allergy in Southern Sweden: A 21-Year Retrospective Study. Acta Derm. Venereol. 2018, 98, 938–942. [Google Scholar] [CrossRef] [PubMed]
  199. European Chemicals Agency (ECHA). Available online: https://echa.europa.eu (accessed on 10 May 2023).
  200. Generally Recognized as Safe (GRAS). Available online: https://www.fda.gov/food/food-ingredients-packaging/generally-recognized-safe-gras (accessed on 10 May 2023).
  201. Api, A.M.; Belsito, D.; Botelho, D.; Bruze, M.; Burton, G.A.; Buschmann, J.; Cancellieri, M.A.; Dagli, M.L.; Date, M.; Dekant, W.; et al. Update to RIFM Fragrance Ingredient Safety Assessment, Eugenol, CAS Registry Number 97-53-0. Food Chem. Toxicol. 2022, 163, 113027. [Google Scholar] [CrossRef]
  202. Api, A.M.; Belsito, D.; Botelho, D.; Bruze, M.; Burton, G.A.; Buschmann, J.; Cancellieri, M.A.; Dagli, M.L.; Date, M.; Dekant, W.; et al. RIFM Fragrance Ingredient Safety Assessment, α-Pinene, CAS Registry Number 80-56-8. Food Chem. Toxicol. 2022, 159, 112702. [Google Scholar] [CrossRef]
  203. Nikolić, B.; Vasilijević, B.; Mitić-Ćulafić, D.; Vuković-Gačić, B.; Knežević-Vukćević, J. Comparative Study of Genotoxic, Antigenotoxic and Cytotoxic Activities of Monoterpenes Camphor, Eucalyptol and Thujone in Bacteria and Mammalian Cells. Chem. Biol. Interact. 2015, 242, 263–271. [Google Scholar] [CrossRef]
  204. Koh, T.; Machino, M.; Murakami, Y.; Umemura, N.; Sakagami, H. Cytotoxicity of Dental Compounds towards Human Oral Squamous Cell Carcinoma and Normal Oral Cells. In Vivo 2013, 27, 85–95. [Google Scholar] [PubMed]
  205. Souza, A.C.S.; Silva, L.K.; Queiroz, T.B.; Marques, E.S.; Hiruma-Lima, C.A.; Gaivão, I.O.M.; Maistro, E.L. Citral Presents Cytotoxic and Genotoxic Effects in Human Cultured Cells. Drug Chem. Toxicol. 2020, 43, 435–440. [Google Scholar] [CrossRef] [PubMed]
  206. Wessels, M.; Rimkus, J.; Leyhausen, G.; Volk, J.; Geurtsen, W. Genotoxic Effects of Camphorquinone and DMT on Human Oral and Intestinal Cells. Dent. Mater. 2015, 31, 1159–1168. [Google Scholar] [CrossRef]
  207. Moorthy, B.; Madyastha, P.; Madyastha, K.M. Hepatotoxicity of Pulegone in Rats: Its Effects on Microsomal Enzymes, in Vivo. Toxicology 1989, 55, 327–337. [Google Scholar] [CrossRef]
  208. Ramos, C.A.F.; Sá, R.d.C.d.S.; Alves, M.F.; Benedito, R.B.; de Sousa, D.P.; Diniz, M.d.F.F.M.; Araújo, M.S.T.; de Almeida, R.N. Histopathological and Biochemical Assessment of D-Limonene-Induced Liver Injury in Rats. Toxicol. Rep. 2015, 2, 482–488. [Google Scholar] [CrossRef]
  209. Baldissera, M.D.; Souza, C.F.; Grando, T.H.; Sagrillo, M.R.; De Brum, G.F.; Nascimento, K.; Peres, D.S.; Maciel, M.F.; Silveira, S.O.; Da Luz, S.C.A.; et al. Memory Deficit, Toxic Effects and Activity of Na+, K+-ATPase and NTPDase in Brain of Wistar Rats Submitted to Orally Treatment with Alpha-Terpinene. Environ. Toxicol. Pharmacol. 2016, 46, 1–8. [Google Scholar] [CrossRef]
  210. Huang, F.-M.; Tai, K.-W.; Chou, M.-Y.; Chang, Y.-C. Cytotoxicity of Resin-, Zinc Oxide-Eugenol-, and Calcium Hydroxide-Based Root Canal Sealers on Human Periodontal Ligament Cells and Permanent V79 Cells. Int. Endod. J. 2002, 35, 153–158. [Google Scholar] [CrossRef] [PubMed]
  211. Wojtunik-Kulesza, K.A. Toxicity of Selected Monoterpenes and Essential Oils Rich in These Compounds. Molecules 2022, 27, 1716. [Google Scholar] [CrossRef] [PubMed]
  212. Zárybnický, T.; Boušová, I.; Ambrož, M.; Skálová, L. Hepatotoxicity of Monoterpenes and Sesquiterpenes. Arch. Toxicol. 2018, 92, 1–13. [Google Scholar] [CrossRef] [PubMed]
  213. Pelkonen, O.; Abass, K.; Wiesner, J. Thujone and Thujone-Containing Herbal Medicinal and Botanical Products: Toxicological Assessment. Regul. Toxicol. Pharmacol. 2013, 65, 100–107. [Google Scholar] [CrossRef]
  214. Zámboriné Németh, É.; Thi Nguyen, H. Thujone, a Widely Debated Volatile Compound: What Do We Know about It? Phytochem. Rev. 2020, 19, 405–423. [Google Scholar] [CrossRef]
  215. Committee on Herbal Medicinal Products (HMPC) Public Statement on the Use of Herbal Medicinal Products Containing Thujone; European Medicine Agency: London, UK, 2011.
Table 1. Biological properties and potential mechanisms of action of selected monoterpenes and monoterpenoids. Chemical structures were obtained from PubChem [18].
Table 1. Biological properties and potential mechanisms of action of selected monoterpenes and monoterpenoids. Chemical structures were obtained from PubChem [18].
Compound NameChemical
Structure		OccurrenceBiological
Properties		Potential
Mechanisms
α-PineneMolecules 28 07178 i001Cannabis sativa L.
Curcuma spp. (turmeric)
Daucus carota (wild carrot)
Juniperus spp. (junipers)
Pinophyta spp. (conifers)
Piper nigrum (black pepper) [19,20,21,22]		Analgesic and antinociceptive [23]Interaction with γ-aminobutyric acid type A (GABAA) receptor [23]
Antifungal [24]Ergosterol complexation [25]
Antiviral [26]Interference with the virion envelope or masking of necessary viral structures [26]
α-TerpineolMolecules 28 07178 i002Citrus aurantium ssp. amara (bitter orange)
Melaleuca spp. (tea trees)
Origanium vulgare L. (oregano)
Pinus spp. (pines)
Salvia rosmarinus (rosemary)
Vitex agnus-castus (chaste tree)
Zingiber officinale (ginger) [27,28]		Anti-inflammatory [29]IL-6 inhibition [29]
α-ThujoneMolecules 28 07178 i003Artemisia absinthium (wormwood)
Artemisia herba-alba (white wormwood)
Artemisia tridentata (big sagebrush)
Salvia fruticosa (Greek sage)
Salvia officinalis (common sage)
Tanacetum vulgare (tansy)
Thymus spp. (thymes) [30,31,32]		Anti-inflammatory [31]Inhibition of PMA/I-induced interleukin-6 (IL-6) and interleukin-8 (IL-8) release [31]
Antiviral [30]Unknown
β-PineneMolecules 28 07178 i004Anethum graveolens (dill)
Melaleuca alternifolia (narrow-leaved paperbark)
Pinus spp. (pines)
Salvia fruticosa (Greek sage)
Salvia officinalis (common sage)
Salvia rosmarinus (rosemary)
Vitex agnus-castus (chaste tree) [33]		Anti-cancer [34]Apoptosis [34]
Antiviral [35]Interference with the virion envelope or masking of necessary viral structures [26]
β-ThujoneMolecules 28 07178 i005Artemisia absinthium (wormwood)
Salvia fruticosa (Greek sage)
Salvia officinalis (common sage)
Thuja occidentalis (northern white cedar)
Thymus spp. (thymes) [30,31,36]		Anti-inflammatory [31]Inhibition of PMA/I-induced IL-6 and IL-8 release [31]
Antiviral [30]Unknown
BorneolMolecules 28 07178 i006Daucus carota (wild carrot)
Mentha spicata (spearmint)
Salvia officinalis (common sage)
Salvia rosmarinus (rosemary)
Thymus vulgaris (common thyme)
Zingiber spp. (true gingers) [31,37,38]		Anti-inflammatory [31]Inhibition of PMA/I-induced IL-6 and IL-8 release [31]
CamphorMolecules 28 07178 i007Artemisia annua (sweet wormwood)
Cinnamomum camphora (camphor tree)
Salvia fruticosa (Greek sage)
Salvia officinalis (common sage)
Salvia rosmarinus (rosemary)
Tanacetum vulgare (tansy) [31,38,39,40]		Anti-inflammatory [31]Inhibition of PMA/I-induced IL-6 and IL-8 release [31]
Antiviral [30]Unknown
CarvacrolMolecules 28 07178 i008Lippia origanoides
Origanum Dictamnus (dittany of Crete)
Origanum vulgare (oregano)
Satureja thymbra (savory of Crete)
Thymus capitatus (conehead thyme)
Thymus serpyllum (white thyme)
Thymus vulgaris (common thyme) [41,42]		Analgesic and antinociceptive [43]Interaction with opioid, vanilloid, and glutamate systems [43]
CitronellalMolecules 28 07178 i009Corymbia citriodora (lemon-scented gum)
Cymbopogon spp. (lemongrass)
Ocimum spp. (basil)
Zingiber spp. (gingers) [44,45,46]		Analgesic and antinociceptive [47]Inhibition of substance P release or neurokinin-1 (NK-1) receptor [47]
CitronellolMolecules 28 07178 i010Citrus × deliciosa (Mediterranean Mandarin)
Corymbia citriodora (lemon-scented gum)
Cymbopogon spp. (lemongrass)
Pelargonium spp. (geraniums)
Rosa × damascena (Damask rose)
Rosa gallica (Gallic rose)
Zingiber spp. (gingers) [48,49]		Antifungal [24]Fungal membrane disruption [50,51]
Eucalyptol (1,8-Cineole)Molecules 28 07178 i011Curcuma spp. (turmerics)
Eucalyptus spp. (eucalyptuses)
Mentha pulegium (pennyroyal)
Mentha × piperita (peppermint)
Salvia fruticosa (Greek sage)
Salvia officinalis (common sage) [30,31,52]		Anti-inflammatory [31]Inhibition of PMA/I-induced IL-6 and IL-8 release [31]
Antiviral [26,30]Interference with the virion envelope or masking of necessary viral structures [26]
EugenolMolecules 28 07178 i012Cinnamomum spp. (cinnamon)
Myristica fragrans (nutmeg)
Ocimum spp. (basils)
Pimenta dioica (allspice)
Syzygium aromaticum (clove) [4,53,54]		Antibacterial [55]Biofilm disruption [56]
GeraniolMolecules 28 07178 i013Camellia sinensis (tea plant)
Citrus × deliciosa (Mediterranean Mandarin)
Cymbopogon spp. (lemongrass)
Vitis vinifera (common grape)
Humulus lupulus (hops)
Pelargonium spp. (geraniums)
Rosa spp. (roses)
Zingiber officinale (ginger) [49,57,58]		Anti-cancer [59]Downregulation of nuclear factor kappa B (NF-κB) pathway [59]
Hinokitiol (β-thujaplicin)Molecules 28 07178 i014Chamaecyparis (false cypresses)
Cupressus spp. (cypresses)
Thuja spp. (thujas)
Thujopsis dolabrata [60]		Anti-inflammatory [61]Downregulation of mRNA transcription of proinflammatory cytokine-related genes [61]
Anti-cancer [62]Induction of apoptosis and autophagy [63]
Antibacterial [64]Inhibition of nutrient transport and cell respiration [65]
Antifungal [66]Chelating of fungal intracellular iron and respiration inhibition of fungal cells [66]
IsoborneolMolecules 28 07178 i015Artemisia annua (sweet wormwood)
Curcuma amada (mango ginger)
Cinnamomum camphora (camphor tree)
Salvia fruticosa (Greek sage)
Salvia officinalis (common sage)
Thymus vulgaris (common thyme)
Zingiber officinale (ginger) [67,68]		Antiviral [69]Inhibition of viral glycosylation [69]
LimoneneMolecules 28 07178 i016Anethum graveolens (dill)
Citrus spp. (citruses)
Eucalyptus globulus (southern blue gum)
Melaleuca alternifolia (narrow-leaved paperbark)
Salvia officinalis (common sage)
Salvia rosmarinus (rosemary)
Vitex agnus-castus (chaste tree) [70,71]		Antifungal [24]Disruption of cell wall and cell membrane, which leads to apoptosis [72]; inhibition of adhesion, enzyme secretion, and biofilm formation [73]
Antiviral [35,74]Interference with virion envelope structures or masking of necessary viral structures [35]
MentholMolecules 28 07178 i017Mentha spp. (mints) [75]Antiviral [76]Attenuation of infection by stimulating TRPM8, which blocks TRPV1-mediated mitochondrial fragmentation [76]
MyrtenolMolecules 28 07178 i018Achillea spp. (yarrows)
Eucalyptus spp. (eucalyptuses)
Myrtus communis (common myrtle)
Taxus spp. (yews) [77,78]		Analgesic and antinociceptive [79]Cytokine inhibition and p38 mitogen-activated protein kinase (p38-MAPK) signal transduction pathway in trigeminal ganglia [79]
Perillic acidMolecules 28 07178 i019Perilla frutescens (beefsteak plant) [80,81]Antiviral [82]Inhibition of maturation, fusion, and viral infectivity [82]
Perillyl alcoholMolecules 28 07178 i020Carum carvi (caraway)
Lavandula × intermedia (lavandin)
Mentha piperita (peppermint)
Mentha spicata (spearmint)
Perilla frutescens (beefsteak plant) [83,84,85]		Antiviral [82]Inhibition of maturation, fusion, and viral infectivity [82]
SabineneMolecules 28 07178 i021Artemisia annua (sweet wormwood)
Juniperus communis (common juniper)
Mesosphaerum suaveolens (pignut)
Piper nigrum (black pepper)
Salvia rosmarinus (rosemary)
Vitex agnus-castus (chaste tree)
Zingiber montanum [86,87,88]		Antibacterial [86,89]Inhibition of bacterial adherence, growth and biofilm formation [89]
ThymolMolecules 28 07178 i022Lippia spp.
Nigella sativa (black caraway)
Ocimum spp. (basils)
Origanum spp. (oreganos)
Satureja thymbra (savory of Crete)
Thymus spp. (thymes)
Trachyspermum ammi (ajowan caraway) [90,91]		Antibacterial [92]Biofilm disruption, decrease in virulence [92,93], reduction of viability and metabolic activity, autolysis [93]
Anti-cancer [94]Mitochondria-mediated apoptosis [94]
Antifungal [95]Disruption of cell membrane integrity [95]
Table 2. Overview of the toxicity of selected monoterpenes and their derivatives. IC50—half maximal inhibitory concentration, CC50—50% cytotoxic concentration, LD50—median lethal dose 50.
Table 2. Overview of the toxicity of selected monoterpenes and their derivatives. IC50—half maximal inhibitory concentration, CC50—50% cytotoxic concentration, LD50—median lethal dose 50.
Type of ToxicityCompound NameType of AssayCell Line/AnimalToxicity
CytotoxicityCamphorIn vitroMRC-5 fetal lung fibroblastsIC50 11 mM [203]
EucalyptolIn vitroMRC-5 fetal lung fibroblastsIC50 11 mM [203]
ThujoneIn vitroMRC-5 fetal lung fibroblastsIC50 2.2 mM [203]
EugenolIn vitroPrimary gingival fibroblasts, hemopoietic progenitor cells, human periodontal ligament cellsCC50 > 755 μM [204]
CitralIn vitroPeripheral blood mononuclear cells, human hepatoma cell line HepG2≥50 μg/mL [205]
GenotoxicityCamphorquinoneIn vitroOKF6/TERT-2 telomerase-immortalized cells≥2.5 mM [206]
PulegoneIn vivoMale albino rats (Indian Institute of Science strain)400 mg/kg [207]
HepatotoxicityLimoneneIn vivoMale Wistar rats25 mg/kg, 75 mg/kg [208]
NeurotoxicityThujoneIn vivoRats,
Mice,
Guinea Pigs		LD50 192 mg/kg (rats),
230 mg/kg (mice), 396 mg/kg guinea pigs [205]
α-TerpineneIn vivoFemale Wistar rats≥0.5 mL/kg [209]
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Potocka, W.; Assy, Z.; Bikker, F.J.; Laine, M.L. Current and Potential Applications of Monoterpenes and Their Derivatives in Oral Health Care. Molecules 2023, 28, 7178. https://doi.org/10.3390/molecules28207178

AMA Style

Potocka W, Assy Z, Bikker FJ, Laine ML. Current and Potential Applications of Monoterpenes and Their Derivatives in Oral Health Care. Molecules. 2023; 28(20):7178. https://doi.org/10.3390/molecules28207178

Chicago/Turabian Style

Potocka, Wiktoria, Zainab Assy, Floris J. Bikker, and Marja L. Laine. 2023. "Current and Potential Applications of Monoterpenes and Their Derivatives in Oral Health Care" Molecules 28, no. 20: 7178. https://doi.org/10.3390/molecules28207178

Article Metrics

Back to TopTop