Heliotropium procubens Mill: Taxonomic Significance and Characterization of Phenolic Compounds via UHPLC–HRMS- In Vitro Antioxidant and Enzyme Inhibitory Activities
, Antigoni Cheilari 1, Gokhan Zengin 2, Konstantia Graikou 1, Christos Ganos 1, George-Albert Karikas 3,* and Ioanna Chinou 1,*Round 1
Reviewer 1 Report
Manuscript Number: molecules-2138940
entitled: Heliotropium procubens Mill: Taxonomic significance and characterization of phenolic compounds via UHPLC-HRMS- In vitro antioxidant and enzyme inhibitory activities
This is an interesting scientific study. Therefore, the manuscript is suitable for Molecules after considering the below comments:
- Would you please show the structures of the main secondary metabolites mentioned in the manuscript?
- Table 1. All secondary metabolites should be described using lowercase, not capital letters, such as “Dihydroxybenzoic acid.”
- Plant material. It possibly will be nice to present the main photos of plants mentioned in the manuscript.
- Conclusion part. Please use the whole name of H. procumbens.
Author Response
This is an interesting scientific study. Therefore, the manuscript is suitable for Molecules after considering the below comments:
- Would you please show the structures of the main secondary metabolites mentioned in the manuscript?
- The structures of the isolated secondary metabolites have been introduced in the Supplementary file.
- Table 1. All secondary metabolites should be described using lowercase, not capital letters, such as “Dihydroxybenzoic acid.”
- Lowercase letters have been used for all secondary metabolites in Table
- Plant material. It possibly will be nice to present the main photos of plants mentioned in the manuscript.
- A photo of the aerial parts of the studied plant has been added in the Supplementary file
- Conclusion part. Please use the whole name of H. procumbens.
- The whole name Heliotropium procumbens has been used in the Conclusion part instead of the short procumbens.
Reviewer 2 Report
Structures of the compounds were elucidated using mass spectra and 3 isolated compounds' structures were confirmed by NMR.
# I suggest authors to insert a scheme (s) of those compounds which were structurally determined by mass with fragmentation pattern (Structure to fragments). please see the published articles
1. (supporting file of Separations 2022, 9(12), 400; https://doi.org/10.3390/separations9120400
2. Molecules 2020, 25(24), 5903; https://doi.org/10.3390/molecules25245903
# UPLC spectra should be inserted as a figure and indication should be given for all the compounds with numbering. and retention times.
Author Response
Structures of the compounds were elucidated using mass spectra and 3 isolated compounds' structures were confirmed by NMR.
# I suggest authors to insert a scheme (s) of those compounds which were structurally determined by mass with fragmentation pattern (Structure to fragments). please see the published articles
1.supporting file of Separations 2022, 9(12),400; https://doi.org/10.3390/separations9120400
- Molecules2020, 25(24), 5903; https://doi.org/10.3390/molecules25245903
- Structures of the compounds, which were isolated and structurally determined, with fragmentation pattern, have been added in the Supplementary file.
# UPLC spectra should be inserted as a figure and indication should be given for all the compounds with numbering. and retention times.
- The mass spectra have been inserted as figures and indication with numbering and retention times has been given for all the determined compounds in the Supplementary file.
Round 2
Reviewer 2 Report
The manuscript is now acceptable for publication.
