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Article

Efficient Bioconversion of Stevioside and Rebaudioside A to Glucosylated Steviol Glycosides Using an Alkalihalobacillus oshimesis-Derived Cyclodextrin Glucanotransferase

by
Ruiqin Zhang
1,2,
Ruiqi Tang
3,
Jiahua Bi
2,
Shanshan Shen
2,
Qin Wu
2,
Qihe Chen
1,* and
Yanjun Li
1,2,*
1
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China
2
Key Laboratory of Food and Biological Engineering of Zhejiang Province, Research and Development Department, Hangzhou Wahaha Technology Co., Ltd., Hangzhou Wahaha Group Co., Ltd., Hangzhou 310018, China
3
Key Laboratory of Bioprocess Engineering of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, China
*
Authors to whom correspondence should be addressed.
Molecules 2023, 28(3), 1245; https://doi.org/10.3390/molecules28031245
Submission received: 30 November 2022 / Revised: 13 January 2023 / Accepted: 24 January 2023 / Published: 27 January 2023
(This article belongs to the Special Issue Production and Modification of Functional Compounds by Microbial Cell Factories)
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Abstract

The enzymatic transglycosylation of steviol glycosides can improve the edulcorant quality of steviol glycosides. Cyclodextrin glucanotransferase (CGTase) is one of the most popular glucanotransferases applied in this reaction. Herein, the CGTase-producing strain Alkalihalobacillus oshimensis CGMCC 23164 was isolated from Stevia planting soil. Using mass spectrometry-based secretome profiling, a high-efficiency CGTase that converted steviol glycosides to glucosylated steviol glycosides was identified and termed CGTase-13. CGTase-13 demonstrated optimal transglycosylation activity with 10 g/L steviol glycoside and 50 g/L soluble starch as substrates at <40 °C. Under the above conditions, the conversion rate of stevioside and rebaudioside A, two main components of steviol glycosides, reached 86.1% and 90.8%, respectively. To the best of our knowledge, this is the highest conversion rate reported to date. Compared with Toruzyme® 3.0 L, the commonly used commercial enzyme blends, glucosylated steviol glycosides produced using CGTase-13 exhibited weaker astringency and unpleasant taste, faster sweetness onset, and stronger sweetness intensity. Thus, CGTase provides a novel option for producing high-quality glucosylated steviol glycoside products and has great potential for industrial applications.
Keywords: Alkalihalobacillus oshimensis; cyclodextrin glucanotransferase (CGTase); steviol glycosides; glucosylated steviol glycosides; transglycosylation Alkalihalobacillus oshimensis; cyclodextrin glucanotransferase (CGTase); steviol glycosides; glucosylated steviol glycosides; transglycosylation

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MDPI and ACS Style

Zhang, R.; Tang, R.; Bi, J.; Shen, S.; Wu, Q.; Chen, Q.; Li, Y. Efficient Bioconversion of Stevioside and Rebaudioside A to Glucosylated Steviol Glycosides Using an Alkalihalobacillus oshimesis-Derived Cyclodextrin Glucanotransferase. Molecules 2023, 28, 1245. https://doi.org/10.3390/molecules28031245

AMA Style

Zhang R, Tang R, Bi J, Shen S, Wu Q, Chen Q, Li Y. Efficient Bioconversion of Stevioside and Rebaudioside A to Glucosylated Steviol Glycosides Using an Alkalihalobacillus oshimesis-Derived Cyclodextrin Glucanotransferase. Molecules. 2023; 28(3):1245. https://doi.org/10.3390/molecules28031245

Chicago/Turabian Style

Zhang, Ruiqin, Ruiqi Tang, Jiahua Bi, Shanshan Shen, Qin Wu, Qihe Chen, and Yanjun Li. 2023. "Efficient Bioconversion of Stevioside and Rebaudioside A to Glucosylated Steviol Glycosides Using an Alkalihalobacillus oshimesis-Derived Cyclodextrin Glucanotransferase" Molecules 28, no. 3: 1245. https://doi.org/10.3390/molecules28031245

APA Style

Zhang, R., Tang, R., Bi, J., Shen, S., Wu, Q., Chen, Q., & Li, Y. (2023). Efficient Bioconversion of Stevioside and Rebaudioside A to Glucosylated Steviol Glycosides Using an Alkalihalobacillus oshimesis-Derived Cyclodextrin Glucanotransferase. Molecules, 28(3), 1245. https://doi.org/10.3390/molecules28031245

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