Alkyl Gallates as Potential Antibiofilm Agents: A Review

Round 1

Reviewer 1 Report

The manuscript "Alkyl Gallates as Potential Antibiofilm Agents: A Review" is written as per the journal style. how ever a few minor corrections are required. Please check the attached PDF file with comments.

Several references are missing. Noticed around 123 references, extracted from Pubmed.org. Please update the manuscript using these references (attached pdf file 2).

Comments for author File: Comments.zip

Author Response

Dear reviewer#1

Thank you for the positive feedback on our manuscript. We are grateful for your insightful comments, as these comments helped us improve the quality of the manuscript. We revised the manuscript according to your comments/ suggestions as detailed below.

Comments and Suggestions for Authors

The manuscript "Alkyl Gallates as Potential Antibiofilm Agents: A Review" is written as per the journal style. however, a few minor corrections are required. Please check the attached PDF file with comments.

Authors’ answer: Based on your comments in the PDF file, we revised our manuscript “molecules-2106184” and made the corrections as follows:

• Page 3. We arranged all the OH groups in the chemical structure of figure 2 in the same way.
• Page 4 and Table 1. We double-checked the concentrations of gallic acid (3-5 mg/mL) and, according to the reference No. 21 [Kosuru et al. Curr Microbiol 2021, 78, (11), 3843-3852], they are provided correctly in our manuscript.
• Page 4. We abbreviated names of bacteria as follows: oralis, S. mitis, S. salivarius.
• Page 5. We abbreviated name of Escherichia coli as follows: coli.
• Page 5. We double-checked the concentration of gallic acid in brackets (10 mg/mL) and, according to the reference No. 33 [Teodoro et al. Front Microbiol 2018, 9, 647], it is provided correctly in our manuscript.
• Page 6, Table 1. We double-checked the concentrations of gallic acid (1.32-2.11 mg/mL) and, according to the reference No. 27 [Sherif et al. Front Microbiol 2021, 12, 716627], they are provided correctly in our manuscript.
• Page 6, Table 1. we double-checked the concentration of gallic acid (3 mg/mL) and, according to the reference No. 28 [Sowndarya et al. Curr Microbiol 2020, 77, (11), 545 3339-3354], it is provided correctly in our manuscript.
• Page 6, Table 1. We double-checked the concentration of gallic acid (1024 µg/mL) and, according to the reference No. 34 [Hossain et al. Pathogens 2020, 9, (10)], it is provided correctly in our manuscript.
• Page 6, Table 1. We double-checked the concentration of gallic acid (2.5 mg/mL) and, according to the reference No. 35 [Gobin et al. Int J Mol Sci 2022, 23, (13)], it is provided correctly in our manuscript.
• Page 7. We double-checked the concentration of methyl gallate (6.25 mg/mL) and, according to the reference No. 36 [Hossain et al. Sci Rep 2017, 7, (1), 10618], it is provided correctly in our manuscript.
• Page 11, Table 4. Thank you very much for paying attention to this typo fault. We corrected the unit of concentration mg/mL.

Several references are missing. Noticed around 123 references, extracted from Pubmed.org. Please update the manuscript using these references (attached pdf file 2).

Authors’ answer: The articles in this list either already cited or are for gallate derivatives that do not belong to the alkyl gallates (e.g., epigallocatechin gallate that is not considered alkyl gallate).

Reviewer 2 Report

The article by Rayan et al „Alkyl Gallates as Potential Antibiofilm Agents: A Review“ presents the latest pieces of information regarding the potential use of gallic acid and its esters against microbial biofilms. The submitted paper is well and professionally written, and I have only a few suggestions:

-        Tables: better format/location inside pages (e.g. Tables 1,4 and 5 are separated on two pages in one cell).

-        Table 1 - missing information for Mechanisms of Action. If there are no suggestions by the authors, add (-) with an explanation at the end of the Table - no suggested/reported mechanism in the reference/article.

-        lines 228 and 231 - Fix the taxonomy of S. Typhimurium to S. Typhimurium

Author Response

Dear reviewer#2

Thank you for the positive feedback on our manuscript. We are grateful for your insightful comments, as these comments helped us improve the quality of the manuscript. We revised the manuscript according to your comments/ suggestions as detailed below.

Comments and Suggestions for Authors

The article by Rayan et al „Alkyl Gallates as Potential Antibiofilm Agents: A Review“ presents the latest pieces of information regarding the potential use of gallic acid and its esters against microbial biofilms. The submitted paper is well and professionally written, and I have only a few suggestions:

-        Tables: better format/location inside pages (e.g. Tables 1,4 and 5 are separated on two pages in one cell).

Authors’ answer: Table 1 cannot fit on one page, while tables 4 and 5 can fit on one page. This issue will be dealt in the proof version.  

-        Table 1 - missing information for Mechanisms of Action. If there are no suggestions by the authors, add (-) with an explanation at the end of the Table - no suggested/reported mechanism in the reference/article.

Authors’ answer: we added (-) in table 1 were mechanism of action was not reported and added an explanation that (-) means mechanism of action was not reported in the reference.

-        lines 228 and 231 - Fix the taxonomy of S. Typhimurium to S. Typhimurium

Authors’ answer: We corrected the taxonomic name of S. Typhimurium to S. Typhimurium.