An ESIPT-Based Fluorescent Probe for Aqueous Cu+ Detection through Strip, Nanofiber and Living Cells
Abstract
:1. Introduction
2. Results
2.1. Structural Characterization
2.2. Spectroscopic Properties
2.2.1. Selectivity and Competition
2.2.2. Linear Relationship
2.2.3. Influence Parameters
2.3. Mechanism
2.4. Imaging Applications
2.4.1. Detection and Imaging of Cu+ by RBg on Test Strips
2.4.2. Detection and Imaging of Cu+ by RBg on Solid-State Nanofibers
2.4.3. Detection and Imaging of Cu+ by RBg in Living HeLa Cells
3. Discussion
4. Materials and Methods
4.1. Reagents and Equipments
4.2. Synthesis of Probe RBg
4.3. Spectroscopic Analysis
4.4. Colorimetric Imaging
4.5. Bioimaging
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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t-Test in Fluorescent Analyses (conc.: 10 μmol L−1 RBg, 40 μmol L−1 Metal Ions/Cu+, x: FI586/FI428) | |||||||
of RBg-Metal Ion | of RBg–Metal Ion–Cu+ | p Value | t | df | R2 | 95% Confidence Interval | Significantly Different (p < 0.05) |
0.004976 | 0.1681 | <0.0001 | 122.5 | 32 | 0.9979 | 0.1604–0.1658 | yes |
t-Test in UV-Vis Analyses (conc.: 10 μmol L−1 RBg, 40 μmol L−1 Metal Ions/Cu+, x: Absorbance) | |||||||
of RBg–Metal Ion | of RBg–Metal Ion–Cu+ | p Value | t | df | R2 | 95% Confidence Interval | Significantly Different (p < 0.05) |
3.761 | 44.68 | <0.0001 | 64.29 | 32 | 0.9923 | 39.62–42.21 | yes |
FI586/FI428 | |||||
Content (μmol L−1) | Added (μmol L−1) | Measured (μmol L−1) | Recovery(%) | Average Recovery(%) | RSD(%) |
1 | 1 | 2.0332 | 101.66% | 99.31% | 2.192% |
1 | 1 | 2.0604 | 103.02% | ||
1 | 1 | 1.9470 | 97.35% | ||
1 | 2 | 2.9187 | 97.29% | ||
1 | 2 | 2.9496 | 98.32% | ||
1 | 2 | 3.0051 | 100.17% | ||
1 | 4 | 4.9279 | 98.56% | ||
1 | 4 | 5.0325 | 100.65% | ||
1 | 4 | 4.8370 | 96.74% | ||
Absorbance | |||||
Content (μmol L−1) | Added (μmol L−1) | Measured (μmol L−1) | Recovery(%) | Average Recovery(%) | RSD(%) |
1 | 1 | 1.9577 | 97.88% | 100.25% | 2.094% |
1 | 1 | 200.14 | 100.07% | ||
1 | 1 | 208.56 | 104.28% | ||
1 | 2 | 3.0328 | 101.09% | ||
1 | 2 | 2.9613 | 98.71% | ||
1 | 2 | 2.9415 | 98.05% | ||
1 | 4 | 4.9723 | 99.45% | ||
1 | 4 | 5.0160 | 100.32% | ||
1 | 4 | 5.1220 | 102.44% |
RSD in RBg–Cu+ Fluorescent Analyses (conc.: 10 μmol L−1 RBg, 40 μmol L−1 Cu+, x: FI586/FI428) | |||||||
x1 | x2 | x3 | x4 | x5 | x6 | ± s | RSD% |
43.0265 | 43.2000 | 44.0230 | 45.1236 | 45.2350 | 45.2057 | 44.3023 ± 1.0278 | 2.3198% |
RSD in RBg–Cu+ UV-Vis Analyses (conc.: 10 μmol L−1 RBg, 40 μmol L−1 Cu+, x: Absorbance) | |||||||
x1 | x2 | x3 | x4 | x5 | x6 | ± s | RSD% |
0.1204 | 0.1256 | 0.1293 | 0.1295 | 0.1300 | 0.1317 | 0.1278 ± 0.004118 | 3.2232% |
t-Tests in RBg–Cu+ Fluorescent Analyses (conc.: 10 μmol L−1 RBg, Varied Cu+, x: FI586/FI428) | ||||||
1 μmol L−1 Cu+ | 3 μmol L−1 Cu+ | 5 μmol L−1 Cu+ | F | p Value | Statistically Significant (p < 0.05) | R2 |
x1 5.0413 | x1 19.5498 | x1 30.9045 | 4810 | 0.0002 | yes | 0.9996 |
x2 5.3454 | x2 18.9978 | x2 31.5612 | ||||
x3 5.0779 | x3 19.4712 | x3 31.1623 | ||||
t-Tests in RBg–Cu+ UV-Vis Analyses (conc.: 10 μmol L−1 RBg, Varied Cu+, x: Absorbance) | ||||||
1 μmol L−1 Cu+ | 3 μmol L−1 Cu+ | 5 μmol L−1 Cu+ | F | p Value | Significant Diff. among Means (p < 0.05) | R2 |
x1 0.0222 | x1 0.0724 | x1 0.1305 | 1032 | <0.0001 | yes | 0.9971 |
x2 0.0235 | x2 0.0698 | x2 0.1369 | ||||
x3 0.0234 | x3 0.0685 | x3 0.1279 |
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Cheng, Z.; Jin, X.; Liu, Y.; Zheng, L.; He, H. An ESIPT-Based Fluorescent Probe for Aqueous Cu+ Detection through Strip, Nanofiber and Living Cells. Molecules 2023, 28, 3725. https://doi.org/10.3390/molecules28093725
Cheng Z, Jin X, Liu Y, Zheng L, He H. An ESIPT-Based Fluorescent Probe for Aqueous Cu+ Detection through Strip, Nanofiber and Living Cells. Molecules. 2023; 28(9):3725. https://doi.org/10.3390/molecules28093725
Chicago/Turabian StyleCheng, Zhao, Xilang Jin, Yinggang Liu, Lei Zheng, and Hao He. 2023. "An ESIPT-Based Fluorescent Probe for Aqueous Cu+ Detection through Strip, Nanofiber and Living Cells" Molecules 28, no. 9: 3725. https://doi.org/10.3390/molecules28093725