Visible-Light-Induced Diselenide-Crosslinked Polymeric Micelles for ROS-Triggered Drug Delivery
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis and Characterization
2.2. Self-Assembly Behavior of MPEG-b-PUSe-b-MPEG Block Copolymers
2.3. Visible-Light-Induced Diselenide-Crosslinked MPEG-b-PUSe-b-MPEG Micelles
2.4. The Structural Stability of the Micelles
2.5. ROS-Induced Disassembly Behavior of Diselenide-Crosslinked Micelles (CLMs)
2.6. DSC Analysis
2.7. In Vitro Drug Release Behavior of DOX-Loaded Micelles
2.8. Cytotoxicities of Diselenide-Crosslinked Micelles (CLMs)
2.9. Cellular Uptake of DOX-Loaded Micelles
3. Materials and Methods
3.1. Materials
3.2. Instrumentation
3.3. Synthesis of the Functional Monomer DiSe
3.4. Synthesis of MPEG-b-PUSe-b-MPEG Triblock Copolymers
3.5. Preparation of Polymeric Micelles and Determination of Critical Micelle Concentration (CMC)
3.6. Preparation of Diselenide Core-Crosslinked Micelles
3.7. Structural Stability of Micelles
3.8. ROS-Triggered Disassembly of Diselenide-Crosslinked Micelles
3.9. In Vitro Drug Loading and Release
3.10. MTT Assay
3.11. Cellular Uptake Measurements
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Nanocarrier | Release Medium | k | n | R2 | Release Mechanism |
---|---|---|---|---|---|
NCLMs | 0 mM H2O2 | 12.25 | 0.34 | 0.93 | pseudo-Fickian |
CLMs | 0 mM H2O2 | 5.74 | 0.31 | 0.98 | pseudo-Fickian |
CLMs | 25 mM H2O2 | 12.00 | 0.58 | 0.99 | Anomalous transport |
CLMs | 50 mM H2O2 | 16.70 | 0.65 | 0.99 | Anomalous transport |
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Cheng, X.; Li, H.; Sun, X.; Xu, T.; Guo, Z.; Du, X.; Li, S.; Li, X.; Xing, X.; Qiu, D. Visible-Light-Induced Diselenide-Crosslinked Polymeric Micelles for ROS-Triggered Drug Delivery. Molecules 2024, 29, 3970. https://doi.org/10.3390/molecules29163970
Cheng X, Li H, Sun X, Xu T, Guo Z, Du X, Li S, Li X, Xing X, Qiu D. Visible-Light-Induced Diselenide-Crosslinked Polymeric Micelles for ROS-Triggered Drug Delivery. Molecules. 2024; 29(16):3970. https://doi.org/10.3390/molecules29163970
Chicago/Turabian StyleCheng, Xinfeng, Huixian Li, Xiaomeng Sun, Tianxu Xu, Zhenzhen Guo, Xianchao Du, Shuai Li, Xuyang Li, Xiaojing Xing, and Dongfang Qiu. 2024. "Visible-Light-Induced Diselenide-Crosslinked Polymeric Micelles for ROS-Triggered Drug Delivery" Molecules 29, no. 16: 3970. https://doi.org/10.3390/molecules29163970