Light Emission from Fe2+-EGTA-H2O2 System Depends on the pH of the Reaction Milieu within the Range That May Occur in Cells of the Human Body
Abstract
:1. Introduction
1.1. Fenton Systems as a Tool for Determination of Anti-(•OH) Activity and Limiting Buffers Interactions
1.2. Properties of Fe2+-EGTA-H2O2 System Generating Ultra-Weak Chemiluminescence
1.3. Aims of the Study
2. Results
2.1. Effect of Singlet Oxygen (O2 (1Δg)) Generating System (H2O2-NaOCl) on the Luminescence Signal Recorded by Luminometer with Photomultiplier Spectrum from 380 nm to 630 nm
2.2. Effect of pH of Reaction Milieu on Light Emission from Fe2+-EGTA-H2O2 System
3. Discussion
3.1. Contribution of 634 nm Photons Derived from Singlet Oxygen Decay to Maximal Signal Generated by the Fe2+-EGTA-H2O2 System
3.2. Effect of pH of Phosphate Buffer on Light Emission from the Fe2+-EGTA-H2O2 System
4. Materials and Methods
4.1. Chemicals and Solutions
4.2. Effect of pH of Reaction Milieu on the Light Emission by Fe2+-EGTA-H2O2 System
4.3. Effect of H2O2–NaOCl System on the Luminescence Signal Recorded by Luminometer with Photomultiplier Spectrum from 380 nm to 630 nm
4.4. Statistical Analyses
5. Conclusions
- Enhancement and inhibition of •OH production in lower and higher pH, respectively.
- Formation of insoluble and non-reactive Fe(OH)3 at neutral and alkaline environment.
- Enhancement of •OH production by phosphates at weakly acidic and neutral environments.
- Suppression of O2•-production in acidic environment with decreased intensity of Fe2+-EGTA complex regeneration.
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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No. | Sample | pH of Reaction Milieu (10 mmol/L PB) pH = 6.8 | pH of Reaction Milieu (10 mmol/L PB) pH = 6.6 |
---|---|---|---|
1. | Complete system H2O2-NaOCl | 926 ± 245 (878; 218) * | 809 ± 124 (827; 105) *,† |
2. | Complete system with O2 (1Δg) scavenger-H2O2-NaN3-NaOCl | 1073 ± 105 (1102; 96) | 930 ± 93 (974; 161) |
3. | Incomplete system I H2O2-NaCl | 628 ± 74 (651; 120) | 641 ± 78 (653; 129) |
4. | Incomplete system II H2O-NaOCl | 651 ± 80 (695; 133) | 654 ± 85 (669; 131) |
5. | H2O-NaN3-NaCl | 640 ± 46 (648; 50) | 636 ± 54 (643; 84) |
6. | H2O-NaCl | 609 ± 60 (630; 107) | 661 ± 104 (659; 101) |
7. | NaCl | 640 ± 47 (649; 65) | 635 ± 56 (653; 121) |
No. | Sample | Working Solutions Added to Luminometer Tube (µL) | ||||
---|---|---|---|---|---|---|
A-PB | B-EGTA | C-FeSO4 | D-H2O | E-H2O | ||
1. | Complete system | 940 | 20 | 20 | 100 | 0 |
2. | Incomplete system I | 960 | 0 | 20 | 100 | 0 |
3. | Incomplete system II | 960 | 20 | 0 | 100 | 0 |
4. | H2O2 alone | 980 | 0 | 0 | 100 | 0 |
5. | Fe2+ + EGTA without H2O2 | 940 | 20 | 20 | 0 | 100 |
6. | Medium alone | 980 | 0 | 0 | 0 | 100 |
No. | Sample | Volumes of Working Solutions Added to Luminometer Tube (μL) | |||||
---|---|---|---|---|---|---|---|
A-PB | B-H2O2 | C-H2O | D-NaN3 | E-NaOCl | F-NaCl | ||
1. | Complete system | 880 | 100 | 0 | 0 | 100 | 0 |
2. | Incomplete system I | 880 | 0 | 100 | 0 | 100 | 0 |
3. | Complete system + NaN3 | 780 | 100 | 0 | 100 | 100 | 0 |
Additional control | |||||||
4. | Incomplete system II | 880 | 100 | 0 | 0 | 0 | 100 |
5. | Medium alone | 880 | 0 | 100 | 0 | 0 | 100 |
6. | Medium + NaN3 | 780 | 0 | 100 | 100 | 0 | 100 |
7. | PB alone | 980 | 0 | 0 | 0 | 0 | 100 |
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Sasak, K.; Nowak, M.; Wlodarczyk, A.; Sarniak, A.; Tryniszewski, W.; Nowak, D. Light Emission from Fe2+-EGTA-H2O2 System Depends on the pH of the Reaction Milieu within the Range That May Occur in Cells of the Human Body. Molecules 2024, 29, 4014. https://doi.org/10.3390/molecules29174014
Sasak K, Nowak M, Wlodarczyk A, Sarniak A, Tryniszewski W, Nowak D. Light Emission from Fe2+-EGTA-H2O2 System Depends on the pH of the Reaction Milieu within the Range That May Occur in Cells of the Human Body. Molecules. 2024; 29(17):4014. https://doi.org/10.3390/molecules29174014
Chicago/Turabian StyleSasak, Krzysztof, Michal Nowak, Anna Wlodarczyk, Agata Sarniak, Wieslaw Tryniszewski, and Dariusz Nowak. 2024. "Light Emission from Fe2+-EGTA-H2O2 System Depends on the pH of the Reaction Milieu within the Range That May Occur in Cells of the Human Body" Molecules 29, no. 17: 4014. https://doi.org/10.3390/molecules29174014