Ellagitannin Content in Extracts of the Chestnut Wood Aesculus
Abstract
:1. Introduction
2. Results and Discussion
2.1. Recovery of Ellagitannins Extracted Using Different Extraction Techniques
2.2. Effect of the Extract on WM-266-4 Cells
3. Materials and Methods
3.1. Plant Material
3.2. Chemicals
3.3. Extractions
3.3.1. Ultrasound Extraction (UE)
3.3.2. Soxhlet Extraction (SE)
3.3.3. Cold Extraction (CE)
3.3.4. Supercritical Fluid Extraction (SFE)
3.4. Determination of Total Phenol Content and Antioxidant Assays
3.5. Identification of Isolated Compounds Using HPLC
3.6. Antimicrobial and Anticarcinogenic Potential of the Extract
3.7. Statistical Evaluation of Results
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
SCF40/250 + EtOH | Supercritical extraction with CO2 at 40 °C and 250 bar with the cosolvent ethanol |
SCF40/250 + (EtOH + H2O) | Supercritical extraction with CO2 at 40 °C and 25 bar with the cosolvent ethanol + water |
SCF40/250 + H2O | Supercritical extraction with CO2 at 40 °C and 250 bar with the cosolvent water |
SCF40/250 + Ac | Supercritical extraction at 40 °C and 250 bar with the cosolvent acetone |
SCF40/250 + (Ac + H2O) | Supercritical extraction with CO2 at 40 °C and 250 bar with the cosolvent acetone + water |
UE (EtOH) | Ultrasound extraction with the solvent ethanol |
UE (EtOH + H2O) | Ultrasound extraction with the solvent ethanol + water |
UE (H2O) | Ultrasound extraction with the solvent water |
UE (Ac) | Ultrasound extraction with the solvent acetone |
UE (Ac + H2O) | Ultrasound extraction with the solvent acetone + water |
CM (EtOH) | Cold maceration with the solvent ethanol |
CM (EtOH + H2O) | Cold maceration with the solvent ethanol + water |
CM (H2O) | Cold maceration with the solvent water |
CM (Ac) | Cold maceration with the solvent acetone |
CM (Ac + H2O) | Cold maceration with the solvent acetone + water |
SOX (EtOH) | Soxhlet extraction with the solvent ethanol |
SOX (EtOH + H2O) | Soxhlet extraction with the solvent ethanol + water |
SOX (H2O) | Soxhlet extraction with the solvent water |
SOX (Ac) | Soxhlet extraction with the solvent acetone |
SOX (Ac + H2O) | Soxhlet extraction with the solvent acetone + water |
Appendix A
SCF40/250 + EtOH | SCF40/250 + H2O | SCF40/250 + (EtOH + H2O) | SCF40/250 + Ac | SCF40/250 + (Ac + H2O) | |
---|---|---|---|---|---|
vescalin | 4.89 | 13.13 | 9.14 | 0.22 | 9.06 |
castalin | 9.58 | 20.41 | 1.83 | ND | 7.39 |
vescalagin | 14.36 | 40.86 | 21.28 | 0.83 | 81.48 |
castalagin | 9.70 | 28.21 | 14.68 | 0.84 | 34.66 |
UE (EtOH) | UE (H2O) | UE (EtOH + H2O) | UE (Ac) | UE (Ac + H2O) | |
---|---|---|---|---|---|
vescalin | 7.05 | 73.71 | 38.05 | 35.37 | 37.71 |
castalin | 9.65 | 75.81 | 41.94 | 24.64 | 26.21 |
vescalagin | 7.88 | 2.71 | 42.79 | 144.13 | 163.85 |
castalagin | 5.73 | 23.06 | 29.42 | 60.32 | 69.47 |
CM (EtOH) | CM (H2O) | CM (EtOH + H2O) | CM (Ac) | CM (Ac + H2O) | |
---|---|---|---|---|---|
vescalin | 7.57 | 62.14 | 39.02 | 18.67 | 46.73 |
castalin | 9.60 | 52.35 | 41.30 | 9.15 | 21.66 |
vescalagin | 9.12 | 29.34 | 34.71 | 95.04 | 131.16 |
castalagin | 6.15 | 17.20 | 23.28 | 45.85 | 58.04 |
SOX (EtOH) | SOX (H2O) | SOX (EtOH + H2O) | SOX (Ac) | SOX (Ac + H2O) | |
---|---|---|---|---|---|
vescalin | 3.30 | 36.30 | 22.74 | 17.54 | 28.87 |
castalin | 17.50 | 34.40 | 37.72 | 14.88 | 22.79 |
vescalagin | 5.50 | 52.41 | 30.49 | 134.30 | 175.35 |
castalagin | 9.33 | 30.41 | 27.75 | 52.62 | 72.62 |
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Žitek Makoter, T.; Knez Marevci, M.; Knez, Ž. Ellagitannin Content in Extracts of the Chestnut Wood Aesculus. Molecules 2024, 29, 4015. https://doi.org/10.3390/molecules29174015
Žitek Makoter T, Knez Marevci M, Knez Ž. Ellagitannin Content in Extracts of the Chestnut Wood Aesculus. Molecules. 2024; 29(17):4015. https://doi.org/10.3390/molecules29174015
Chicago/Turabian StyleŽitek Makoter, Taja, Maša Knez Marevci, and Željko Knez. 2024. "Ellagitannin Content in Extracts of the Chestnut Wood Aesculus" Molecules 29, no. 17: 4015. https://doi.org/10.3390/molecules29174015