UHPLC-QTOF-MS-Based Targeted Metabolomics Provides Novel Insights into the Accumulative Mechanism of Soil Types on the Bioactive Components of Salvia miltiorrhiza
Abstract
:1. Introduction
2. Results
2.1. Comparative Analysis of Different Soil Factors from the Five Soils
2.2. Changes in Salvianolic Acid B and Tanshinone IIA in Different Soils
2.3. The Biosynthetic Pathways of Salvianolic Acid B and Tanshinone I of SMB Planted in Different Soils Were Different
2.4. Chemometrics Analysis
2.5. Correlation Analysis of SMB Components and Soil Factors
2.6. Soil Drivers of Water- and Lipid-Soluble Constituents of SMBs
3. Discussion
4. Materials and Methods
4.1. Study Sites
4.2. Soil Collection and Experimental Set-Up
4.3. Plant Materials
4.4. Soil Sampling and Elemental Analysis
4.5. Extraction and UHPLC-QTOF-MS Analysis
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Soil Site | Salvianolic Acid B (%) | Total Tanshinone (%) | Salvianolic Acid B: Total Tanshinone |
---|---|---|---|
A | 7.06 ± 0.16 d | 1.00 ± 0.01 a | 7.03 |
B | 7.11 ± 0.18 d | 0.76 ± 0.03 b | 9.34 |
C | 8.43 ± 0.04 c | 0.95 ± 0.04 a | 8.91 |
D | 9.15 ± 0.07 b | 1.03 ± 0.03 a | 8.87 |
E | 9.69 ± 0.19 a | 0.61 ± 0.01 c | 15.99 |
Number | Patient ID | tR (min) | Mass Ion (m/z) | Data-Dependent MS Data (m/z) | Identidication | Cite |
---|---|---|---|---|---|---|
1 | Sm4 | 3.49 | [M − H]− 197.05 | 174.96, 146.97 | Danshensu | [24,30,31] |
2 | Sm7 | 7.00 | [M − H]− 179.03 | 164.93, 135.04, 104.96 | Caffeic acid | [30,31] |
3 | Sm9 | 9.86 | [M + Na]+ 337.10 | 302.97, 286.99, 247.00, 231.05 | Salvianolic acid F | [30,31] |
4 | Sm10 | 11.56 | [M − H]− 735.16 | 735.16, 294.89, 174.96, 146.97 | Hydrosalvianolic acid B | [30] |
5 | Sm15 | 12.70 | [M − H]− 359.08 | 197.04, 161.02 | Rosmarinic acid | [24,30,31,32,33,34] |
6 | Sm16 | 13.06 | [M − H]− 537.11 | 493.12, 294.89, 174.96, 146.97 | Lithospermic acid | [24,31,34] |
7a | Sm18 | 13.81 | [M − H]− 717.16 | 635.08, 294.89, 174.96, 146.97 | Salvianolic acid B | [30,31,32,33] |
8 | Sm19 | 14.23 | [M + Na]+ 741.15 | 425.09, 205.06 | Salvianolic acid E | [24] |
9 | Sm20 | 14.8 | [M − H]− 717.15 | 551.13, 362.88, 226.92, 174.96 | Iso salvianolic acid B | [30] |
10 | Sm24 | 15.61 | [M − H]− 551.12 | 424.85, 248.90, 226.92, 180.91 | Methyl salvianolic acid I/H | [30] |
11 | Sm27 | 16.54 | [M + Na]+ 319.10 | 301.08, 279.10, 261.06,173.01 | Tanshinone VI | [30] |
12 | Sm31 | 18.21 | [M + H]+ 274.27 | 212.12, 173.01, 125.99, 110.01 | Tanshinone IIB | [30,31,35] |
13 | Sm33 | 19.50 | [M + H]+ 341.14 | 212.12, 173.01, 143.00, 125.99 | Methyldihydronortanshinonate | [30] |
14 | Sm40 | 20.37 | [2M + H]+ 619.31 | 441.45, 321.15, 205.06 | Tanshinaldehyde | [30,31] |
15 | Sm43 | 21.19 | [M + H]+ 279.10 | 212.12, 173.01, 143.00, 125.99 | 15,16-Dihydrotanshinone I | [30] |
16 | Sm46 | 22.01 | [M + H]+ 281.11 | 173.01, 125.99 | Trijuganone B | [30,31,35] |
17 | Sm47 | 22.50 | [M + H]+ 339.12 | 212.12, 173.01, 143.00, 125.99 | Methyl tanshinonate | [30] |
18 | Sm48 | 24.16 | [M + H]+ 297.15 | 279.00, 251.00 | Cryptotanshinone | [29,30,33] |
19 | Sm49 | 25.22 | [M + H]+ 279.10 | 212.12, 173.01, 143.00, 125.99 | 1,2-Dihydrotanshinone I | [30] |
20 | Sm50 | 25.36 | [M + H]+ 279.10 | 212.12, 173.01, 143.00, 125.99 | 3,4-dihydrotanshinone I | [30] |
21 a | Sm53 | 27.24 | [M + H]+ 295.15 | 125.99 | Tanshinone IIA | [24,29,30,31,32,33,35] |
22 | Sm54 | 28.11 | [M + H]+ 283.17 | 212.12, 202.09, 125.99, 110.01 | Miltirone | [31,35] |
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Hou, M.; Gao, D.; Chen, W.; Jiang, W.; Yu, D.; Li, X. UHPLC-QTOF-MS-Based Targeted Metabolomics Provides Novel Insights into the Accumulative Mechanism of Soil Types on the Bioactive Components of Salvia miltiorrhiza. Molecules 2024, 29, 4016. https://doi.org/10.3390/molecules29174016
Hou M, Gao D, Chen W, Jiang W, Yu D, Li X. UHPLC-QTOF-MS-Based Targeted Metabolomics Provides Novel Insights into the Accumulative Mechanism of Soil Types on the Bioactive Components of Salvia miltiorrhiza. Molecules. 2024; 29(17):4016. https://doi.org/10.3390/molecules29174016
Chicago/Turabian StyleHou, Mengmeng, Dan Gao, Weixu Chen, Wenjun Jiang, Dade Yu, and Xiwen Li. 2024. "UHPLC-QTOF-MS-Based Targeted Metabolomics Provides Novel Insights into the Accumulative Mechanism of Soil Types on the Bioactive Components of Salvia miltiorrhiza" Molecules 29, no. 17: 4016. https://doi.org/10.3390/molecules29174016