Isolation of Anti-Prion Compounds from Curcuma phaeocaulis Valeton Extract
Abstract
:1. Introduction
2. Results and Discussion
2.1. Activity-Guided Identification of Active Fractions in the Cp Extract for Anti-Prion Activity
2.2. Isolation of Major Compounds from the Cp Extract by CPC
2.3. Determination of Chemical Identity
2.4. Evaluation of the Anti-Prion Activity of the Major Compounds That Constitute the HX Fraction
3. Materials and Methods
3.1. Apparatus and Materials
3.2. Plant Material and Preparation of the Cp Extract
3.3. Preparation of the Two-Phase Solvent System
3.4. CPC and Preparative HPLC Separation of the HX Fraction
3.5. HPLC Analysis
3.6. Structural Elucidation
3.7. Measurement of PrPSc Levels and Cytotoxicity in ScN2a Cells
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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n-HX:ethanol:water (v/v/v) | K-Values | ||
---|---|---|---|
Curcumenone (1) | Curcumenol (2) | Furanodienone (3) | |
10:9:1 | 0.27 | 0.44 | 0.81 |
10:8:2 | 0.20 | 0.41 | 1.18 |
10:7:3 | 0.26 | 0.58 | 2.14 |
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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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Kim, J.; Lee, H.; Kim, H.M.; Kim, J.H.; Byun, S.; Lee, S.; Kim, C.Y.; Ryou, C. Isolation of Anti-Prion Compounds from Curcuma phaeocaulis Valeton Extract. Molecules 2024, 29, 4034. https://doi.org/10.3390/molecules29174034
Kim J, Lee H, Kim HM, Kim JH, Byun S, Lee S, Kim CY, Ryou C. Isolation of Anti-Prion Compounds from Curcuma phaeocaulis Valeton Extract. Molecules. 2024; 29(17):4034. https://doi.org/10.3390/molecules29174034
Chicago/Turabian StyleKim, Jaehyeon, Hakmin Lee, Hye Mi Kim, Ji Hoon Kim, Sanghoon Byun, Sungeun Lee, Chul Young Kim, and Chongsuk Ryou. 2024. "Isolation of Anti-Prion Compounds from Curcuma phaeocaulis Valeton Extract" Molecules 29, no. 17: 4034. https://doi.org/10.3390/molecules29174034