Application of PLGA-PEG-PLGA Nanoparticles to Percutaneous Immunotherapy for Food Allergy
Abstract
:1. Introduction
2. Results
2.1. Evaluation of Physical Properties of Prepared Nanoparticles
2.2. Ex Vivo Measurement of Intracutaneous HEL Accumulation
2.3. Ex Vivo Observation of the Intradermal Permeation Pathway of FITC-HEL
2.4. Results of the Measurement of Antibody Titer in Blood after in Vivo Percutaneous Immunization Experiment
3. Materials and Methods
3.1. Materials
3.2. Animals
3.3. Preparation of HEL-Loaded PLGA and PLGA-PEG-PLGA Nanoparticle Formulations
3.4. Preparation of FITC-HEL-Loaded PLGA and PLGA-PEG-PLGA Nanoparticle Formulations
3.5. Evaluation of Physical Properties of PLGA and PLGA-PEG-PLGA Nanoparticle Formulations Loaded with HEL and FITC-HEL
3.6. Drug Skin Retention Study of FITC-HEL-Loaded PLGA and PLGA-PEG-PLGA Nanoparticle Formulations
3.7. Observation of Intradermal Permeation Pathway of FITC-HEL
3.8. In Vivo Percutaneous Immunization Experiments
3.9. Data Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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HEL-Loaded CS-PLGA NPs | FITC-HEL-Loaded CS-PLGA NPs | HEL-Loaded PLGA-PEG-PLGA NPs | FITC-HEL-Loaded PLGA-PEG-PLGA NPs | |
---|---|---|---|---|
Mean diameter (nm) | 100 ± 47 | 99 ± 49 | 105 ± 54 | 87 ± 43 |
Zeta potential (mV, I = 5 mM) | 38.4 ± 1.9 | −7.2 ± 0.1 | ||
Yield (%) | 82.9 ± 12.0 | 67.7 ± 2.7 | ||
HEL content in NPs (%) | 8.0 ± 0.8 | 7.9 ± 0.7 | 3.4 ± 0.4 | 3.4 ± 0.9 |
HEL entrapment efficiency (w/w%) | 66.7 ± 7.0 | 65.5 ± 6.1 | 28.7 ± 3.4 | 28.1 ± 7.8 |
Polydispersity index | 0.23 ± 0.01 | 0.25 ± 0.02 | 0.27 ± 0.01 | 0.30 ± 0.01 |
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Sakurai, R.; Iwata, H.; Gotoh, M.; Ogino, H.; Takeuchi, I.; Makino, K.; Itoh, F.; Saitoh, A. Application of PLGA-PEG-PLGA Nanoparticles to Percutaneous Immunotherapy for Food Allergy. Molecules 2024, 29, 4123. https://doi.org/10.3390/molecules29174123
Sakurai R, Iwata H, Gotoh M, Ogino H, Takeuchi I, Makino K, Itoh F, Saitoh A. Application of PLGA-PEG-PLGA Nanoparticles to Percutaneous Immunotherapy for Food Allergy. Molecules. 2024; 29(17):4123. https://doi.org/10.3390/molecules29174123
Chicago/Turabian StyleSakurai, Ryuse, Hanae Iwata, Masaki Gotoh, Hiroyuki Ogino, Issei Takeuchi, Kimiko Makino, Fumio Itoh, and Akiyoshi Saitoh. 2024. "Application of PLGA-PEG-PLGA Nanoparticles to Percutaneous Immunotherapy for Food Allergy" Molecules 29, no. 17: 4123. https://doi.org/10.3390/molecules29174123