Neuroprotective Effect of Flavonoid Agathisflavone in the Ex Vivo Cerebellar Slice Neonatal Ischemia
Abstract
:1. Introduction
2. Results
2.1. The Flavonoid Agathisflavone Prevented the Loss of Oligodendrocyte Processes
2.2. Agathisflavone Prevented White Matter Loss in Cerebellar Slices Subjected to Ischemic Damage
2.3. Agathisflavone Prevents the Loss of OPCs in the Molecular Layer in Cerebellar Slices Subjected to Ischemic Damage
2.4. Flavonoid Agathisflavone Prevented Astrocyte Reactivity in Acute Ischemic Damage in Cerebellar Slices
2.5. Agathisflavone Prevented the Loss of Glutamine Synthetase Expression in Ischemic Damage in Cerebellar Slices
2.6. Agathisflavone Protects Purkinje Cells from Acute Ischemic Damage
2.7. The Expression Profile of Cytokines Related to the Inflammatory Response
3. Discussion
4. Materials and Methods
4.1. Agathisflavone
4.2. Animals and Tissue
4.3. Ex Vivo Cerebellar Slice Preparation and Oxygen–Glucose Deprivation (OGD)
4.4. Immunofluorescence Labeling
4.5. Image Acquisition and Analysis
4.6. Quantitative RT-PCR
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Gene ID | Direct Sequence | Reverse Sequence |
---|---|---|---|
TNF-α | 21926 | GGTGCCTATGTCTCAGCCTCTT | GCCATAGAACTGATGAGAGGGAG |
IL-1β | 16176 | TGGACCTTCCAGGATGAGGACA | GTTCATCTCGGAGCCTGTAGTG |
IL-10 | 16153 | CGGGAAGACAATAACTGCACCC | CGGTTAGCAGTATGTTGTCCAGC |
GAPDH | 14433 | CATCACTGCCACCCAGAAGACTG | ATGCCAGTGAGCTTCCCGTTCAG |
ACTB | 11461 | CATTGCTGACAGGATGCAGAAGG | TGCTGGAAGGTGGACAGTGAGG |
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Carreira, R.B.; dos Santos, C.C.; de Oliveira, J.V.R.; da Silva, V.D.A.; David, J.M.; Butt, A.M.; Costa, S.L. Neuroprotective Effect of Flavonoid Agathisflavone in the Ex Vivo Cerebellar Slice Neonatal Ischemia. Molecules 2024, 29, 4159. https://doi.org/10.3390/molecules29174159
Carreira RB, dos Santos CC, de Oliveira JVR, da Silva VDA, David JM, Butt AM, Costa SL. Neuroprotective Effect of Flavonoid Agathisflavone in the Ex Vivo Cerebellar Slice Neonatal Ischemia. Molecules. 2024; 29(17):4159. https://doi.org/10.3390/molecules29174159
Chicago/Turabian StyleCarreira, Rodrigo Barreto, Cleonice Creusa dos Santos, Juciele Valeria Ribeiro de Oliveira, Victor Diogenes Amaral da Silva, Jorge Maurício David, Arthur Morgan Butt, and Silvia Lima Costa. 2024. "Neuroprotective Effect of Flavonoid Agathisflavone in the Ex Vivo Cerebellar Slice Neonatal Ischemia" Molecules 29, no. 17: 4159. https://doi.org/10.3390/molecules29174159