Fabrication of PLA-Based Nanoneedle Patches Loaded with Transcutol-Modified Chitosan Nanoparticles for the Transdermal Delivery of Levofloxacin
Abstract
:1. Introduction
2. Results and Discussion
2.1. Modified Chitosan Nanoparticles
2.1.1. ATR Spectroscopy
2.1.2. Swelling Efficiency
2.1.3. X-ray Diffraction (XRD)
2.1.4. Particle Size Determination
2.1.5. In Vitro Dissolution of Levofloxacin
2.2. PLA/PEAd Nanoneedles
2.2.1. Nanoneedles’ Morphology
2.2.2. Compression Test
2.2.3. Ex Vivo Studies
3. Materials and Methods
3.1. Materials
3.2. Methods
3.2.1. Synthesis of Transcutol-Modified Chitosan
3.2.2. Preparation of Levo-Loaded Nanoparticles
3.2.3. Preparation of NP-Loaded Nanoneedles
3.2.4. Structure and Properties Investigation
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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NPs Sample Based on Preparation Ratio | Particle Size (nm) | PDI |
---|---|---|
1/1 CS-trans | 620 ± 108 | 0.642 |
1/2 CS-trans | 390 ± 182 | 0.591 |
1/3 CS-trans | 220 ± 84 | 0.345 |
CS-Trans/TPP Weight Ratio | Particle Size (nm) | PDI |
---|---|---|
1/1 | 4520 ± 203 | 0.899 |
2/1 | 220 ± 84 | 0.345 |
4/1 | 3840 ± 405 | 0.563 |
NPs Sample | Particle Size (nm) | PDI | % Drug Loading | % EE |
---|---|---|---|---|
CS/trans-Levo | 435 ± 77 | 0.452 | 8.97 ± 1.27 | 24.5 ± 2.38 |
PLA/PEAd w/w Ratio | Sample |
---|---|
90/10 | S1 |
80/20 | S2 |
70/30 | S3 |
60/40 | S4 |
50/50 | S5 |
Sample | Papp (cm/h) |
---|---|
NNDs/Levo-loaded NPs | 0.20791070 |
NNDs/Levo | 1.90731 × 10−5 |
Levofloxacin API | 0.00130121 |
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Samiotaki, C.; Koumentakou, I.; Christodoulou, E.; Bikiaris, N.D.; Vlachou, M.; Karavas, E.; Tourlouki, K.; Kehagias, N.; Barmpalexis, P. Fabrication of PLA-Based Nanoneedle Patches Loaded with Transcutol-Modified Chitosan Nanoparticles for the Transdermal Delivery of Levofloxacin. Molecules 2024, 29, 4289. https://doi.org/10.3390/molecules29184289
Samiotaki C, Koumentakou I, Christodoulou E, Bikiaris ND, Vlachou M, Karavas E, Tourlouki K, Kehagias N, Barmpalexis P. Fabrication of PLA-Based Nanoneedle Patches Loaded with Transcutol-Modified Chitosan Nanoparticles for the Transdermal Delivery of Levofloxacin. Molecules. 2024; 29(18):4289. https://doi.org/10.3390/molecules29184289
Chicago/Turabian StyleSamiotaki, Christina, Ioanna Koumentakou, Evi Christodoulou, Nikolaos D. Bikiaris, Marilena Vlachou, Evangelos Karavas, Konstantina Tourlouki, Nikolaos Kehagias, and Panagiotis Barmpalexis. 2024. "Fabrication of PLA-Based Nanoneedle Patches Loaded with Transcutol-Modified Chitosan Nanoparticles for the Transdermal Delivery of Levofloxacin" Molecules 29, no. 18: 4289. https://doi.org/10.3390/molecules29184289