Fluorescence Polarization Assay for Infection Diagnostics: A Review
Abstract
:1. Introduction
2. Principles of Fluorescence Polarization Assay
3. Detection of Infectious Diseases by FPA
4. Detection of Antibodies to Viruses and Bacteria by FPA
4.1. Antibodies to Brucellosis
4.2. Antibodies to Salmonella
4.3. Antibodies to Tuberculosis
4.4. Antibodies to Equine Infectious Anemia Virus (EIAV)
4.5. Antibodies to Avian Influenza a Virus (IAV)
4.6. Antibodies to SARS-CoV-2
Disease | Sample | Fluorescent Recognition Element | Ref. |
---|---|---|---|
Brucellosis | Bovine sera | Flu-OPS | [20,34,47,49] * |
Milk | Flu-OPS | [35] | |
Sheep sera | Flu-OPS | [17,36] | |
Pig sera | Flu-OPS | [37] | |
Cervid sera | Flu-OPS | [38] | |
Camel sera | Flu-OPS | [46] | |
Salmonellosis | Egg yolk Chicken sera | Flu-OPS | [21] [53] |
Tuberculosis | Bovine sera | Flu-MPB70 | [21,39] |
Bovine sera | Flu-ESAT-6 protein | [40] | |
Bovine sera | Flu-peptide | [54,56] * | |
Paratuberculosis | Bovine sera | Flu-protein fraction of 35 kDa from Map 3065 | [24] |
Equine infectious anemia virus | Horse sera | Flu-peptide | [23,41,61] * |
Influenza A virus | Chicken and goat sera | Flu-peptide | [58] |
Chicken and goat sera | Recombinant H5 subtype HA Flu-proteins | [65] | |
SARS-CoV-2 Antibody | Human sera | SARS-CoV-2 receptor binding domain (Flu-RBD) protein | [28] |
5. Detection of Virus Particles with the Use of FPA
6. Detection of Nucleic Acid with the Use of FPA
7. Conclusions and Future Trends for the Use of FPA for Infection Detection
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Infectious Agent | Se | Sp | Cut-Off (mP) | Ref. |
---|---|---|---|---|
B. abortus and B. melitensis | 88.7 99.0 | 92.5 95 | 89.9 90 | [20] [34] |
B. melitensis | 95 | 100 | 74.1 | [35] |
B. melitensis | 97.6 | 98.9 | 87 | [36] |
B. suis | 93.5 | 97.2 | 84 | [37] |
B. abortus and B. suis | 99 | 84 | 80 | [38] |
M. bovis | 92.9 | 98.3 | 173.9 | [39] |
96.9 | 64.6 | 127 | [40] | |
Paratuberculosis | 88.5 | 91.4 | 126 | [24] |
Equine infectious anemia virus | 98 | 98 | 98.6 | [21] |
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Eremin, S.A.; Mukhametova, L.I.; Krylov, V.B.; Nifantiev, N.E. Fluorescence Polarization Assay for Infection Diagnostics: A Review. Molecules 2024, 29, 4712. https://doi.org/10.3390/molecules29194712
Eremin SA, Mukhametova LI, Krylov VB, Nifantiev NE. Fluorescence Polarization Assay for Infection Diagnostics: A Review. Molecules. 2024; 29(19):4712. https://doi.org/10.3390/molecules29194712
Chicago/Turabian StyleEremin, Sergei A., Liliya I. Mukhametova, Vadim B. Krylov, and Nikolay E. Nifantiev. 2024. "Fluorescence Polarization Assay for Infection Diagnostics: A Review" Molecules 29, no. 19: 4712. https://doi.org/10.3390/molecules29194712