Next Article in Journal
Correction: Jiang et al. Preparation of Pinoresinol and Dehydrodiconiferyl Alcohol from Eucommiae Cortex Extract by Fermentation with Traditional Mucor. Molecules 2024, 29, 2979
Previous Article in Journal
Synthesis, Characterization, and Evaluation of the Antifungal Properties of 3-Indolyl-3-Hydroxy Oxindole Derivatives Against Plant Pathogenic Fungi
Previous Article in Special Issue
Chemical Conversations
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 30
Issue 5
10.3390/molecules30051080
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

The Absence of a Very Long Chain Fatty Acid (VLCFA) in Lipid A Impairs Agrobacterium fabrum Plant Infection and Biofilm Formation and Increases Susceptibility to Environmental Stressors

by
Iwona Komaniecka
1,*,
Kamil Żebracki
1,
Andrzej Mazur
1,
Katarzyna Suśniak
2,
Anna Sroka-Bartnicka
3,
Anita Swatek
1 and
Adam Choma
1,*
1
Department of Genetics and Microbiology, Institute of Biological Sciences, Maria Curie-Sklodowska University, 20-033 Lublin, Poland
2
Department of Pharmaceutical Microbiology, Medical University of Lublin, Chodźki 1, 20-093 Lublin, Poland
3
Independent Unit of Spectroscopy and Chemical Imaging, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland
*
Authors to whom correspondence should be addressed.
Molecules 2025, 30(5), 1080; https://doi.org/10.3390/molecules30051080 (registering DOI)
Submission received: 30 January 2025 / Revised: 22 February 2025 / Accepted: 24 February 2025 / Published: 26 February 2025
(This article belongs to the Special Issue The 30th Anniversary of Molecules—Recent Advances in Chemical Biology)
Versions Notes

Abstract

The Agrobacterium fabrum C58 is a phytopathogen able to infect numerous species of cultivated and ornamental plants. During infection, bacteria genetically transform plant cells and induce the formation of tumours at the site of invasion. Bacterial cell wall components play a crucial role in the infection process. Lipopolysaccharide is the main component of Gram-negative bacteria's outer leaflet of outer membrane. Its lipophilic part, called lipid A, is built of di-glucosamine backbone substituted with a specific set of 3-hydroxyl fatty acids. A. fabrum incorporates a very long chain hydroxylated fatty acid (VLCFA), namely 27-hydroxyoctacosanoic acid (28:0-(27OH)), into its lipid A. A. fabrum C58 mutants deprived of this component due to mutation in the VLCFA’s genomic region, have been characterised. High-resolution mass spectrometry was used to establish acylation patterns in the mutant’s lipid A preparations. The physiological properties of mutants, as well as their motility, ability to biofilm formation and plant infectivity, were tested. The results obtained showed that the investigated mutants were more sensitive to environmental stress conditions, formed a weakened biofilm, exhibited impaired swimming motility and were less effective in infecting tomato seedlings compared to the wild strain.
Keywords: Agrobacterium fabrum; lipid A; very long chain fatty acids; mass spectrometry; biofilm; FTIR spectroscopy; plant infection Agrobacterium fabrum; lipid A; very long chain fatty acids; mass spectrometry; biofilm; FTIR spectroscopy; plant infection

Share and Cite

MDPI and ACS Style

Komaniecka, I.; Żebracki, K.; Mazur, A.; Suśniak, K.; Sroka-Bartnicka, A.; Swatek, A.; Choma, A. The Absence of a Very Long Chain Fatty Acid (VLCFA) in Lipid A Impairs Agrobacterium fabrum Plant Infection and Biofilm Formation and Increases Susceptibility to Environmental Stressors. Molecules 2025, 30, 1080. https://doi.org/10.3390/molecules30051080

AMA Style

Komaniecka I, Żebracki K, Mazur A, Suśniak K, Sroka-Bartnicka A, Swatek A, Choma A. The Absence of a Very Long Chain Fatty Acid (VLCFA) in Lipid A Impairs Agrobacterium fabrum Plant Infection and Biofilm Formation and Increases Susceptibility to Environmental Stressors. Molecules. 2025; 30(5):1080. https://doi.org/10.3390/molecules30051080

Chicago/Turabian Style

Komaniecka, Iwona, Kamil Żebracki, Andrzej Mazur, Katarzyna Suśniak, Anna Sroka-Bartnicka, Anita Swatek, and Adam Choma. 2025. "The Absence of a Very Long Chain Fatty Acid (VLCFA) in Lipid A Impairs Agrobacterium fabrum Plant Infection and Biofilm Formation and Increases Susceptibility to Environmental Stressors" Molecules 30, no. 5: 1080. https://doi.org/10.3390/molecules30051080

APA Style

Komaniecka, I., Żebracki, K., Mazur, A., Suśniak, K., Sroka-Bartnicka, A., Swatek, A., & Choma, A. (2025). The Absence of a Very Long Chain Fatty Acid (VLCFA) in Lipid A Impairs Agrobacterium fabrum Plant Infection and Biofilm Formation and Increases Susceptibility to Environmental Stressors. Molecules, 30(5), 1080. https://doi.org/10.3390/molecules30051080

Article Metrics

Back to TopTop