Overexpression of a Weed (Solanum americanum) Proteinase Inhibitor in Transgenic Tobacco Results in Increased Glandular Trichome Density and Enhanced Resistance to Helicoverpa armigera and Spodoptera litura
Abstract
:1. Introduction
2. Results and Discussion
2.1. Expression of SaPIN2a in transgenic tobacco plants
2.2. Proteinase inhibitory activities of transgenic SaPIN2a
2.3. Insect-resistant activity of transgenic SaPIN2a against H. armigera and S. litura
2.4. Effect of transgenic SaPIN2a on trichome development in tobacco
3. Experimental Section
3.1. RNA ligase-mediated rapid amplification of 5′ cDNA end (RLM-5′ RACE)
3.2. Binary vectors and plant transformation
3.3. PCR analysis of transgenic plants
3.4. Northern blot and western blot analysis
3.5. Proteinase inhibitory activity assay
3.6. Scanning electron microscopy
3.7. Insect feeding trials
4. Conclusions
Acknowledgments
References
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Mortality (%) | Pupation rate (%) | |||
---|---|---|---|---|
H. armigera | S. litura | H. armigera | S. litura | |
WT | 16.7 ± 3.3 | 43.3 ± 3.3 | 83.8 ± 4.4 | 82.2 ± 1.1 |
P51 | 36.7 ± 3.3 * | 56.7 ± 8.8 * | 47.6 ± 2.4** | 63.9 ± 7.3 * |
S14 | 50.0 ± 5.8 ** | 66.7 ± 6.7 * | 26.1 ± 3.9** | 50.0 ± 0.0 * |
F161 | 40.0 ± 5.8 ** | 86.7 ± 6.7 ** | 32.1 ± 6.6** | 33.3 ± 16.7 ** |
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Luo, M.; Wang, Z.; Li, H.; Xia, K.-F.; Cai, Y.; Xu, Z.-F. Overexpression of a Weed (Solanum americanum) Proteinase Inhibitor in Transgenic Tobacco Results in Increased Glandular Trichome Density and Enhanced Resistance to Helicoverpa armigera and Spodoptera litura. Int. J. Mol. Sci. 2009, 10, 1896-1910. https://doi.org/10.3390/ijms10041896
Luo M, Wang Z, Li H, Xia K-F, Cai Y, Xu Z-F. Overexpression of a Weed (Solanum americanum) Proteinase Inhibitor in Transgenic Tobacco Results in Increased Glandular Trichome Density and Enhanced Resistance to Helicoverpa armigera and Spodoptera litura. International Journal of Molecular Sciences. 2009; 10(4):1896-1910. https://doi.org/10.3390/ijms10041896
Chicago/Turabian StyleLuo, Ming, Zhaoyu Wang, Huapeng Li, Kuai-Fei Xia, Yinpeng Cai, and Zeng-Fu Xu. 2009. "Overexpression of a Weed (Solanum americanum) Proteinase Inhibitor in Transgenic Tobacco Results in Increased Glandular Trichome Density and Enhanced Resistance to Helicoverpa armigera and Spodoptera litura" International Journal of Molecular Sciences 10, no. 4: 1896-1910. https://doi.org/10.3390/ijms10041896