Inactivation and Unfolding of the Hyperthermophilic Inorganic Pyrophosphatase from Thermus thermophilus by Sodium Dodecyl Sulfate
Abstract
:1. Introduction
2. Results and Discussion
2.1. Inactivation of T-PPase by SDS
2.2. Structural Changes of PPase Induced by SDS
3. Experimental Section
3.1. Materials
3.2. Protein Expression and Purification
3.3. PPase Activity Assay
3.4. Determination of Inhibition Constants
3.5. Spectroscopy
4. Conclusions
Acknowledgments
References
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Mu, H.; Zhou, S.-M.; Xia, Y.; Zou, H.; Meng, F.; Yan, Y.-B. Inactivation and Unfolding of the Hyperthermophilic Inorganic Pyrophosphatase from Thermus thermophilus by Sodium Dodecyl Sulfate. Int. J. Mol. Sci. 2009, 10, 2849-2859. https://doi.org/10.3390/ijms10062849
Mu H, Zhou S-M, Xia Y, Zou H, Meng F, Yan Y-B. Inactivation and Unfolding of the Hyperthermophilic Inorganic Pyrophosphatase from Thermus thermophilus by Sodium Dodecyl Sulfate. International Journal of Molecular Sciences. 2009; 10(6):2849-2859. https://doi.org/10.3390/ijms10062849
Chicago/Turabian StyleMu, Hang, Sheng-Mei Zhou, Yong Xia, Hechang Zou, Fanguo Meng, and Yong-Bin Yan. 2009. "Inactivation and Unfolding of the Hyperthermophilic Inorganic Pyrophosphatase from Thermus thermophilus by Sodium Dodecyl Sulfate" International Journal of Molecular Sciences 10, no. 6: 2849-2859. https://doi.org/10.3390/ijms10062849