Co-localization of Carbonic Anhydrase and Phosphoenol-pyruvate Carboxylase and Localization of Pyruvate Kinase in Roots and Hypocotyls of Etiolated Glycine max Seedlings
Abstract
:1. Introduction
2. Results
2.1. CA activity and histochemical localization of CA activity in etiolated G. max seedlings
2.2. Immunohistochemical detection of CA and PEPC protein
2.3. Spatial patterns of GmCA1, GmPEPC7 and GmPK3 transcripts in primary and lateral roots of etiolated G. max seedlings
3. Discussion
4. Conclusions
5. Experimental Section
5.1. Plant material and growth conditions
5.2. Characterization of a cDNA clone coding for GmPK3
5.3. RT-PCR analysis of GmPK3 and GmPEPC7 transcript levels
5.4. In situ hybridization
5.5. Histochemical localization of CA activity
5.6. Total CA activity of G. max organ extracts
5.7. Protein extraction from G. max tissues and Western blot of PEPC and CA
5.8. Immunohistochemical detection of PEPC and CA
References and Notes
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CA activitiy [μmole H+min−1 (mg protein)−1] | ||
---|---|---|
plumule | hypocotyl | root |
ND (a) | 85.527±8.785 (b) | 216.363±51.969 (c) |
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
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Dimou, M.; Paunescu, A.; Aivalakis, G.; Flemetakis, E.; Katinakis, P. Co-localization of Carbonic Anhydrase and Phosphoenol-pyruvate Carboxylase and Localization of Pyruvate Kinase in Roots and Hypocotyls of Etiolated Glycine max Seedlings. Int. J. Mol. Sci. 2009, 10, 2896-2910. https://doi.org/10.3390/ijms10072896
Dimou M, Paunescu A, Aivalakis G, Flemetakis E, Katinakis P. Co-localization of Carbonic Anhydrase and Phosphoenol-pyruvate Carboxylase and Localization of Pyruvate Kinase in Roots and Hypocotyls of Etiolated Glycine max Seedlings. International Journal of Molecular Sciences. 2009; 10(7):2896-2910. https://doi.org/10.3390/ijms10072896
Chicago/Turabian StyleDimou, Maria, Anca Paunescu, Georgios Aivalakis, Emmanouil Flemetakis, and Panagiotis Katinakis. 2009. "Co-localization of Carbonic Anhydrase and Phosphoenol-pyruvate Carboxylase and Localization of Pyruvate Kinase in Roots and Hypocotyls of Etiolated Glycine max Seedlings" International Journal of Molecular Sciences 10, no. 7: 2896-2910. https://doi.org/10.3390/ijms10072896