Genetic Differentiation between Natural and Hatchery Stocks of Japanese Scallop (Mizuhopecten yessoensis) as Revealed by AFLP Analysis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Scallop Samples
2.2. DNA Isolation and AFLP Analysis
2.3. Data Analysis
3. Results
3.1. AFLP Polymorphism and Genetic Variation
3.2. Population Structure
4. Discussion
5. Conclusions
Acknowledgments
References
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No. | Abbreviation | Location | Nature of seed | Culture method |
---|---|---|---|---|
1 | GI | Guanglu Island | artificial | suspended |
2 | ZI | Zhangzi Island | artificial | bottom |
3 | XM | Xiaochang Mountain | artificial | suspended |
4 | LSB | Lingshui Bridge | artificial | suspended |
5 | LSK | Lüshun Kou | natural | suspended |
6 | JA | Aomori, Japan | natural | suspended |
7 | RU | Vladivostok, Russia | natural | bottom |
Adaptors & Primers | Sequences |
---|---|
Adaptors | |
MesI adaptor | GACGATGAGTCCTGAG/TACTCAGGACTCAT |
EcoRI adaptor | CTCGTAGACTGCGTACC/CTGACGCATGGTTAA |
Primers | |
E-AAC/M-CCA | GACTGCGTACCAATTCAAC/GATGAGTCCTGAGTAACCA |
E-ACA/M-CTC | GACTGCGTACCAATTCACA/GATGAGTCCTGAGTAACTC |
E-ATC/M-CCT | GACTGCGTACCAATTCATC/GATGAGTCCTGAGTAACCT |
E-ATC/M-CTT | GACTGCGTACCAATTCATC/GATGAGTCCTGAGTAACTT |
E-ATG/M-CCT | GACTGCGTACCAATTCATG/GATGAGTCCTGAGTAACCT |
E-ATG/M-CTG | GACTGCGTACCAATTCATG/GATGAGTCCTGAGTAACTG |
Population ID | Number of sample (N) | Average heterozygosity (H) | Percentage of polymorphic loci (P) |
---|---|---|---|
GI | 48 | 0.2285 | 64.29% |
ZI | 47 | 0.2317 | 62.01% |
XM | 48 | 0.2300 | 62.66% |
LSB | 47 | 0.2442 | 67.21% |
LSK | 49 | 0.2380 | 65.58% |
JA | 49 | 0.2596 | 72.08% |
RU | 44 | 0.2116 | 61.04% |
Overall | 332 | 0.2601 | 91.58% |
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Liu, W.-D.; Li, H.-J.; Bao, X.-B.; Gao, X.-g.; Li, Y.-f.; He, C.-B.; Liu, Z.-J. Genetic Differentiation between Natural and Hatchery Stocks of Japanese Scallop (Mizuhopecten yessoensis) as Revealed by AFLP Analysis. Int. J. Mol. Sci. 2010, 11, 3933-3941. https://doi.org/10.3390/ijms11103933
Liu W-D, Li H-J, Bao X-B, Gao X-g, Li Y-f, He C-B, Liu Z-J. Genetic Differentiation between Natural and Hatchery Stocks of Japanese Scallop (Mizuhopecten yessoensis) as Revealed by AFLP Analysis. International Journal of Molecular Sciences. 2010; 11(10):3933-3941. https://doi.org/10.3390/ijms11103933
Chicago/Turabian StyleLiu, Wei-Dong, Hong-Jun Li, Xiang-Bo Bao, Xiang-gang Gao, Yun-feng Li, Chong-Bo He, and Zhan-Jiang Liu. 2010. "Genetic Differentiation between Natural and Hatchery Stocks of Japanese Scallop (Mizuhopecten yessoensis) as Revealed by AFLP Analysis" International Journal of Molecular Sciences 11, no. 10: 3933-3941. https://doi.org/10.3390/ijms11103933