A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae)
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
4. Conclusions
Acknowledgements
References
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Locus | Primer sequence (5′–3′) | Repeat motif | Size range (bp) | Ta (°C) | GenBank Accession No. |
---|---|---|---|---|---|
LG2 * | F: ATGCTACACTTTCATCTCGGTA R: CGGTTGGAAGCTAAAATG | (GA)14 | 255–271 | 57 | JN625264 |
LG5 * | F: GTAGGGTAAGAGTGGGTTG R: GTTTGGGAGTGGTTTGAT | (CT)10- (CA)14 | 166–170 | 60 | JN625265 |
LG6 | F: TCTTGGTCCTTTACTGGC R: TCATGCGAAATTCTCCAC | (CA)12 | 214 | 60 | JN625266 |
LG10 * | F: GAAGCCCATTCCTGTTAC R: AAGCATTAGGCAAAGTCA | (GAA)4- (CA)11 | 153–165 | 61 | JN625267 |
LG11 * | F: TAGAAACATACCCACCTG R: ACACTTCCAGAAAACCTC | (GAA)7 | 280–295 | 61 | JN625268 |
LG12 | F: ATCATTCAGGCTGACACG R: CAAATCCCAATACTTTCG | (GT)8 (GA)11 | 189 | 54 | JN625269 |
LG13 * | F: CTACTTCTTGATCCTTCT R: TAGCATGTTGTAAATGTC | (CT)6 (CA)10 | 132–138 | 55 | JN625270 |
LG14 | F: AAAAAAGAAGACGAGAGCA R: GCCGCAGATGTAAATAGG | (AG)15 | 219 | 60 | JN625271 |
LG15 | F: CCAAAGTGCCAACAAAGA R: GAGGAGGGGGAACCAGAG | (TC)29 | 269 | 51 | JN625272 |
LG17 * | F: TCGGGATCATGTAGTTATT R: GTTTACTTTTACCATGCTTCTA | (CA)12- (GA)21 | 148–160 | 59 | JN625273 |
LG22 | F: GAATCGGACGAACTTTCT R: TGTAGCCTATCCTACCTCA | (GAA)7 | 150 | 61 | JN625274 |
LG24 | F: GAGAAAGGTGGACTACTGT R: TCGGAGTTCTGATGGGAT | (TTC)8 | 181 | 59 | JN625275 |
LG25 * | F: CTTCACTTGGACTGGAGC R: TTGAATTTTGTGCTTGGT | (CA)7- (GA)9 | 283–289 | 50 | JN625276 |
LG26 * | F: TTACAAATTGCAAGGAGG R: CCACTTCATCTTCCCTTA | (GA)13 | 108–118 | 57 | JN625277 |
LG27 * | F: GTGATTTTGCTCTCTCTGTCTCTTT R: AGTGGTTACAATGCTGGT | (TG)11 | 84–90 | 63 | JN625278 |
LG28 | F: AAAGCAGGACAAAGAACAC R: ATTGAGGACGAAGCAGAA | (GA)14 | 194 | 52 | JN625279 |
LG30 | F: ATCGATTATTCACTCACG R: AGTAGTAACCTTGCCAGA | (CT)29 | 162 | 52 | JN625280 |
LG31 | F: CCCAACCAAATGAGATGA R: TTGGCTCTGGTAATAAAGG | (AC)5 (AG)25 | 209 | 54 | JN625281 |
LG33 * | F: GCGGACATCAATTTTAGTACTCTAT R: TGTCTCCAGGACCAAAGG | (GA)29 | 124–133 | 57 | JN625282 |
Population NMD (N = 12) | Population BLG (N = 12) | |||||
---|---|---|---|---|---|---|
Locus | NA | HE | HO | NA | HE | HO |
LG2 | 5 | 0.619 | 0.333 | 4 | 0.608 | 0.333 |
LG5 | 2 | 0.507 | 0.000 * | 2 | 0.289 | 0.333 |
LG10 | 3 | 0.420 | 0.000 * | 4 | 0.710 | 0.166 * |
LG11 | 3 | 0.565 | 0.166 * | 3 | 0.409 | 0.083 * |
LG13 | 3 | 0.619 | 1.000 * | 3 | 0.561 | 1.000 * |
LG17 | 4 | 0.655 | 0.416 | 5 | 0.641 | 0.333 * |
LG25 | 2 | 0.344 | 0.083 | 3 | 0.467 | 0.250 |
LG26 | 3 | 0.565 | 0.166 * | 3 | 0.554 | 0.083 * |
LG27 | 2 | 0.391 | 0.500 | 5 | 0.760 | 0.333 * |
LG33 | 3 | 0.681 | 0.000 * | 4 | 0.739 | 0.500 |
Mean | 3.000 | 0.536 | 0.266 | 3.600 | 0.573 | 0.341 |
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Zhou, W.; Li, D.; Wang, H. A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae). Int. J. Mol. Sci. 2011, 12, 6743-6748. https://doi.org/10.3390/ijms12106743
Zhou W, Li D, Wang H. A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae). International Journal of Molecular Sciences. 2011; 12(10):6743-6748. https://doi.org/10.3390/ijms12106743
Chicago/Turabian StyleZhou, Wei, Dezhu Li, and Hong Wang. 2011. "A Set of Novel Microsatellite Markers Developed for a Distylous Species Luculia gratissima (Rubiaceae)" International Journal of Molecular Sciences 12, no. 10: 6743-6748. https://doi.org/10.3390/ijms12106743