Effects of Newcastle Disease Virus Strains AF2240 and V4-UPM on Cytolysis and Apoptosis of Leukemia Cell Lines
Abstract
:1. Introduction
2. Results
2.1. Cytotolytic Effects of NDV on Normal Cells and Myelomonocytic Leukemia Cells
2.2. BrdU Cell Proliferation Assay
2.3. Trypan Blue Exclusion Assay
2.4. DNA Fragmentation Assay
2.5. Effects of NDV on Cell Cycle Distribution
2.6. Activation of Caspase-8, an Initiator Caspase for the Extrinsic Pathway
2.7. Activation of Caspase-9, an Initiator Caspase for the Intrinsic Pathway
2.8. Activation of Effector/Executioner Caspases (Caspase-3/7)
3. Materials and Methods
3.1. Propagation and Purification of NDV Strains AF2240 and V4-UPM
3.2. Cells and Cell Culture
3.3. Cell Viability and Proliferation Assessment
3.3.1. MTT Cytotoxicity Assay
3.3.2. BrdU Proliferation Assay
3.3.3. Trypan Blue Exclusion Assay
3.4. DNA Fragmentation Assay
3.5. Analysis of Cellular DNA Content Using Propidium Iodide
3.6. Analysis of Caspase-3/7, 8 and 9 Activities
3.7. Statistical Analysis
4. Discussions
Acknowledgments
References
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Cell Lines | AF 2240 | V4-UPM | Doxorubicin | Arabinocytocine (Ara-C) |
---|---|---|---|---|
WEHI-3B | 2 ± 0.2 | 8 ± 0.2 | 0.8 ± 0.6 | 1.0 ± 0.13 |
HL-60 | 25 ± 0.3 | 110 ± 0.8 | 1.8 ± 0.1 | 0.3 ± 0.05 |
CEM-SS | 16 ± 0.6 | 64 ± 0.5 | 3.5 ± 0.1 | 3.8 ± 0.30 |
3T3 | NO CD50 | NO CD50 | 7.0 ± 0.4 | 2.8 ± 0.20 |
PBMC | NO CD50 | NO CD50 | 11.0 ± 0.8 | 7.0 ± 0.80 |
Mouse spleen lymphocyte | NO CD50 | NO CD50 | 6.5 ± 0.2 | 4.7 ± 0.6 |
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Alabsi, A.M.; Bakar, S.A.A.; Ali, R.; Omar, A.R.; Bejo, M.H.; Ideris, A.; Ali, A.M. Effects of Newcastle Disease Virus Strains AF2240 and V4-UPM on Cytolysis and Apoptosis of Leukemia Cell Lines. Int. J. Mol. Sci. 2011, 12, 8645-8660. https://doi.org/10.3390/ijms12128645
Alabsi AM, Bakar SAA, Ali R, Omar AR, Bejo MH, Ideris A, Ali AM. Effects of Newcastle Disease Virus Strains AF2240 and V4-UPM on Cytolysis and Apoptosis of Leukemia Cell Lines. International Journal of Molecular Sciences. 2011; 12(12):8645-8660. https://doi.org/10.3390/ijms12128645
Chicago/Turabian StyleAlabsi, Aied M., Siti Aishah Abu Bakar, Rola Ali, Abdul Rahman Omar, Mohd Hair Bejo, Aini Ideris, and Abdul Manaf Ali. 2011. "Effects of Newcastle Disease Virus Strains AF2240 and V4-UPM on Cytolysis and Apoptosis of Leukemia Cell Lines" International Journal of Molecular Sciences 12, no. 12: 8645-8660. https://doi.org/10.3390/ijms12128645