Lycopene Enhances Antioxidant Enzyme Activities and Immunity Function in N-Methyl-N′-nitro-N-nitrosoguanidine–Induced Gastric Cancer Rats
Abstract
:1. Introduction
2. Methods
2.1. Extraction of Lycopene
2.2. Animals
2.3. Experimental Design
2.4. Biochemical Analysis
2.4.1. SOD Activity Assay
2.4.2. CAT Activity Assay
2.4.3. Assay of Glutathione Peroxidase Activity
2.4.4. The Tissues MDA Concentration
2.4.5. IL-2, IL-4, IL-6, IL-10, TNF-α, IgA, IgG and IgM Levels
2.5. Statistical Analysis
3. Results
4. Discussion
5. Conclusion
Acknowledgments
References
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Group | Body weight (g) | |
---|---|---|
Before experiment | After experiment | |
I | 187.5 ± 15.3 | 223.1 ± 19.4 |
II | 188.2 ± 17.8 | 202.3 ± 22.1a |
III | 185.2 ± 16.2 | 209.4 ± 18.6 |
IV | 184.8 ± 19.1 | 211.5 ± 23.5c |
V | 186.7 ± 19.3 | 218.2 ± 20.6c |
Group | SOD (U/mg) | CAT (U/mg) | GSH-Px (U/mg) | MDA (U/mg) |
---|---|---|---|---|
I | 182.16 ± 17.29 | 29.85 ± 2.17 | 31.63 ± 3.72 | 8.27 ± 0.69 |
II | 98.26 ± 7.27b | 16.72 ± 1.99b | 18.92 ± 2.41b | 12.18 ± 0.93b |
III | 126.13 ± 13.83d | 19.82 ± 1.68d | 23.86 ± 2.83d | 10.73 ± 0.82c |
IV | 162.95 ± 19.04d | 22.47 ± 3.01d | 27.91 ± 3.09d | 9.32 ± 0.79d |
V | 188.29 ± 20.11d | 28.14 ± 3.12d | 30.61 ± 3.21d | 8.05 ± 0.91d |
Group | SOD (U/mg) | CAT (U/mg) | GSH-Px (U/mg) | MDA (U/mg) |
---|---|---|---|---|
I | 200.14 ± 27.31 | 38.12 ± 4.42 | 27.29 ± 3.28 | 6.39 ± 0.72 |
II | 117.32 ± 14.21b | 21.72 ± 3.17b | 20.13 ± 2.41b | 9.31 ± 1.32b |
III | 152.71 ± 17.08d | 26.92 ± 3.05d | 23.81 ± 2.64c | 8.14 ± 0.93c |
IV | 170.48 ± 19.36d | 29.33 ± 3.14d | 26.18 ± 2.91d | 7.53 ± 0.84d |
V | 194.28 ± 22.13d | 37.11 ± 4.25d | 29.52 ± 3.28d | 6.54 ± 0.74d |
Group | IL-2 (ng/mL) | IL-4 (ng/mL) | IL-10 (ng/mL) | IL-6 (ng/mL) | TNF-α (ng/mL) |
---|---|---|---|---|---|
I | 6.04 ± 0.83 | 21.54 ± 1.83 | 90.91 ± 10.63 | 70.63 ± 8.42 | 4.13 ± 0.67 |
II | 4.83 ± 0.51b | 15.62 ± 1.72b | 62.76 ± 3.84b | 89.17 ± 9.59b | 2.69 ± 0.49b |
III | 5.42 ± 0.63c | 17.42 ± 2.08c | 74.93 ± 6.20d | 82.61 ± 10.07c | 3.08 ± 0.44d |
IV | 5.82 ± 0.62d | 18.96 ± 2.11d | 82.17 ± 5.81d | 78.49 ± 9.48d | 3.51 ± 0.51d |
V | 6.11 ± 0.73d | 20.51 ± 2.54d | 89.53 ± 5.39d | 75.37 ± 8.37d | 3.99 ± 0.53d |
Group | IgG (g/L) | IgA (g/L) | IgM (g/L) |
---|---|---|---|
I | 5.03 ± 0.31 | 0.64 ± 0.05 | 0.71 ± 0.08 |
II | 3.52 ± 0.03b | 0.43 ± 0.04b | 0.55 ± 0.06b |
III | 3.97 ± 0.04c | 0.52 ± 0.06c | 0.62 ± 0.07c |
IV | 4.58 ± 0.05d | 0.59 ± 0.06d | 0.68 ± 0.05d |
V | 4.89 ± 0.06d | 0.63 ± 0.07d | 0.74 ± 0.06d |
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Luo, C.; Wu, X.-G. Lycopene Enhances Antioxidant Enzyme Activities and Immunity Function in N-Methyl-N′-nitro-N-nitrosoguanidine–Induced Gastric Cancer Rats. Int. J. Mol. Sci. 2011, 12, 3340-3351. https://doi.org/10.3390/ijms12053340
Luo C, Wu X-G. Lycopene Enhances Antioxidant Enzyme Activities and Immunity Function in N-Methyl-N′-nitro-N-nitrosoguanidine–Induced Gastric Cancer Rats. International Journal of Molecular Sciences. 2011; 12(5):3340-3351. https://doi.org/10.3390/ijms12053340
Chicago/Turabian StyleLuo, Cong, and Xian-Guo Wu. 2011. "Lycopene Enhances Antioxidant Enzyme Activities and Immunity Function in N-Methyl-N′-nitro-N-nitrosoguanidine–Induced Gastric Cancer Rats" International Journal of Molecular Sciences 12, no. 5: 3340-3351. https://doi.org/10.3390/ijms12053340