A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
4. Conclusions
Acknowledgements
References
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Locus | Primer sequence (5′-3′) | Repeat motif | Size (bp) | Ta (°C) | GenBank Accession No. |
---|---|---|---|---|---|
MH 01 * | F: GCACTGGCTTAGTTCTTT R: TGTTTGTCTCAGGGGTAT | (CT)12(CCCT)3(CT)4 | 213 | 60 | JN871296 |
MH 02 * | F: AAACGAGCAGAAACTGGA R: GCATTGTCCAACCCTTTA | (TG)11(AG)9 | 153 | 60 | JN871297 |
MH 03 * | F: CAACTTCCCAAATTCTGC R: TACCCAACACTTTCTCACCA | (CT)16 | 322 | 61 | JN871298 |
MH 04 * | F: TTTCATTCCGTAACCTT R: GCCAAAAACTTGTATAATTC | (CT)33 | 198 | 57 | JN871299 |
MH 05 * | F: TCGTACAATTTTGTTGCTCT R: CATTCCTCAATAAACCCATA | (TG)12 | 130 | 61 | JN871300 |
MH 06 * | F: CTCTATCTGTCGCATTCTTC R: TCTAGTAATACTATCCTCCACC | (TC)19 | 201 | 61 | JN871301 |
MH 07 * | F: ACCTCGGATAGAAAACAGC R: GTCGTATATAAAGTAAAGCATTGCC | (GA)4(CA)6(GA)14 | 284 | 57 | JN871302 |
MH 08 * | F: TGAACTCCCCAGTCCCCACT R: TCGGTCCACAATCATCACAAAA | (AG)22 | 187 | 62 | JN871303 |
MH 09 * | F: TGGTCATCAACTATTTTCCCTC R: ATGTATTCATTCAGGTATAGAGAGA | (TC)14 | 153 | 57 | JN871304 |
MH 10 * | F: GTCCTCCATAGCCACAAA R: TACTATAACTGATTTTCAAATTTCC | (GAA)13 | 130 | 59 | JN871305 |
MH 11 * | F: GTCCTAGTGTACTTACCCATCA R: GACAGCAATGCTCCGACT | (CA)14 | 192 | 61 | JN871306 |
MH 12 | F: CAATTCTTGAGCCACTTT R: GCTTCTGCCAATGTTTAG | (GA)13 | 208 | 56 | JN871307 |
MH 13 | F: AAGAAGGTGAAGGAGGCT R: AGATGGTCCCAATGTAGC | (TC)12(GT)7(CT)4 | 262 | 49 | JN871308 |
MH 14 | F: GGGTTCAAGGCTCAGGAC R: CATCCCATTGCCAACATC | (GA)15 | 221 | 49 | JN871309 |
MH 15 | F: TATGCTTGGGCAGGAATG R: AACGCTGTGCTATCACTGTCTT | (GA)21 | 201 | 54 | JN871310 |
MH 16 | F: TTTTAGTAGGGTTCACCG R: CCCACTCCTGGATGTTTG | (CT)32 | 192 | 54 | JN871311 |
MH 17 | F: ATTCTGTATCTGGCTCAC R: GTCTTTCAAGCATAAAGA | (CT)14(CA)11 | 279 | 55 | JN871312 |
MH 18 | F: ACGAGCAGAAACTGGAAA R: GCATTGTCCAACCCTTTA | (GT)11(GA)9 | 153 | 58 | JN871313 |
MH 19 | F: TCTACTTCGGTTCAGGGTT R: GCTTCCGCTGTAATGGTT | (TC)25(AC)5 | 283 | 61 | JN871314 |
MH 20 | F: ATGACTCCACATAGCAAACA R: CTGGGCTGATCCTAAACA | (AG)20 | 233 | 63 | JN871315 |
MH 21 | F: GGGAAATGGTTCTTTATGGT R: TCATCATGGGTTGGGTTAT | (CT)21 | 142 | 59 | JN871316 |
MH 22 | F: CATCAACTCACGATTGCA R: CGTTATGTTTCTTTTCTCCC | (TA)6(GA)16 | 236 | 55 | JN871317 |
MH 23 | F: GCAGGTGAGAATGCAAAT R: AAGCAGCATCACAGTTCC | (AG)27(TG)4 | 157 | 59 | JN871318 |
MH 24 | F: GCTTGTTTTGATGGTTGT R: TAGGCGAATGATGCTTAT | (CA)13(GA)25 | 424 | 55 | JN871319 |
Population 1 (N = 12) | Population 2 (N = 12) | |||||
---|---|---|---|---|---|---|
Locus | NA | HO | HE | NA | HO | HE |
MH 01 | 4 | 0.833 | 0.684 | 3 | 0.666 | 0.489 |
MH 02 | 2 | 0.333 | 0.463 | 3 | 0.250 | 0.539 |
MH 03 | 3 | 0.000 | 0.652 | 4 | 0.000 | 0.681 |
MH 04 | 3 | 0.250 | 0.561 | 3 | 0.250 | 0.684 |
MH 05 | 4 | 0.416 | 0.655 | 4 | 0.583 | 0.771 |
MH 06 | 3 | 0.166 | 0.681 | 4 | 0.500 | 0.753 |
MH 07 | 2 | 0.333 | 0.463 | 5 | 0.583 | 0.768 |
MH 08 | 3 | 0.166 | 0.594 | 4 | 0.500 | 0.699 |
MH 09 | 4 | 0.250 | 0.587 | 4 | 0.583 | 0.655 |
MH 10 | 2 | 0.250 | 0.431 | 2 | 0.666 | 0.463 |
MH 11 | 3 | 0.666 | 0.648 | 3 | 0.333 | 0.594 |
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Ma, H.; Wang, L.; Wan, Y.; Li, H.; Li, Z.; Liu, X.; Liang, N.; Li, W. A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China. Int. J. Mol. Sci. 2012, 13, 534-539. https://doi.org/10.3390/ijms13010534
Ma H, Wang L, Wan Y, Li H, Li Z, Liu X, Liang N, Li W. A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China. International Journal of Molecular Sciences. 2012; 13(1):534-539. https://doi.org/10.3390/ijms13010534
Chicago/Turabian StyleMa, Hong, Lan Wang, Youming Wan, Hongzhe Li, Zhenghong Li, Xiuxian Liu, Ning Liang, and Wenjuan Li. 2012. "A Set of Novel Microsatellite Markers Developed for Luculia yunnanensis (Rubiaceae), an Endangered Plant Endemic to Yunnan, China" International Journal of Molecular Sciences 13, no. 1: 534-539. https://doi.org/10.3390/ijms13010534