The Bone-Protective Effect of Genistein in the Animal Model of Bilateral Ovariectomy: Roles of Phytoestrogens and PTH/PTHR1 Against Post-Menopausal Osteoporosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents
Medicine
Materials
2.2. Experimental Protocols
2.3. Determination of Bone Mineral Density
2.4. Femoral Mechanical Testing
2.5. Serum and Urine Mineral Analysis
2.6. Serum Biochemistry
2.7. RNA Isolation and Reverse Transcription-PCR
2.8. Western Blot
2.9. Immunohistochemistry
2.10. Statistical Analysis
3. Results
3.1. Body Weights
3.2. Bone Mineral Density
3.3. Mechanical Properties
3.4. Serum and Urine Minerals and PTH Levels
3.5. Effects of Genistein on Serum Bone Markers
3.6. PTHR1 Expressions in Femoral Tissues
3.7. Localization and Distribution of PTHR1 in the Kidney
4. Discussion
5. Conclusion
References
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0 week | 1 week | 2 weeks | 4 weeks | 8 weeks | 12 weeks | |
---|---|---|---|---|---|---|
sham | 229.6 ± 7.1 | 248.3 ± 9.7 | 242.5 ± 10.3 | 252.8 ± 14.8 | 303.3 ± 23.7 | 324.3 ± 35.2 |
OVX | 223.4 ± 19.1 | 254.7 ± 28.2 | 273.4 ± 15.3 a | 298.0 ± 32.6 a | 310.7 ± 23.0 | 325.3 ± 31.7 |
NIL | 226.3 ± 15.3 | 246.2 ± 12.9 | 250.1 ± 4.8 b | 264.9 ± 21.9 b | 277.2 ± 14.7 b | 297.1 ± 27.5 b |
GENL | 228.7 ± 11.2 | 250.9 ± 10.3 | 265.2 ± 25.3 | 276.1 ± 24.2 b | 280.7 ± 35.9 b | 312.8 ± 26.7 |
GENM | 225.5 ± 13.3 | 262.2 ± 13.4 | 268.0 ± 14.7 | 284.6 ± 17.5 b | 329.7 ± 20.5 b | 347.7 ± 31.2 b |
GENH | 227.4 ± 9.8 | 255.0 ± 18.1 | 261.4 ± 8.7 | 275.2 ± 10.5 b | 312.1 ± 22.4 | 328.8 ± 36.3 |
Group | Serum Ca (mmol/L) | Serum P (mmol/L) | Urine Ca/Cr | Urine P/Cr | PTH (pg/mL) |
---|---|---|---|---|---|
Sham | 2.15 ± 0.53 | 2.39 ± 0.24 | 0.30 ± 0.03 | 4.47 ± 0.23 | 108.54 ± 12.36 |
OVX | 1.72 ± 0.36 a | 2.00 ± 0.39 a | 0.58 ± 0.02 a | 5.85 ± 0.30 a | 5.14 ± 17.49 a |
NIL | 1.90 ± 0.63 b | 2.21 ± 0.32 b | 0.35 ± 0.01 b | 4.82 ± 0.43 b | 83.43 ± 20.78 |
GENL | 1.83 ± 0.40 b | 2.45 ± 0.38 b | 0.49 ± 0.06 b | 5.48 ± 0.14 b | 78.62 ± 18.95 |
GENM | 2.09 ± 0.31 b | 2.63 ± 0.84 b | 0.37 ± 0.05 b | 5.00 ± 0.36 b | 80.23 ± 22.54 |
GENH | 2.12 ± 0.42 b | 2.71 ± 0.75 b | 0.32 ± 0.02 b | 4.64 ± 0.20 b | 95.37 ± 21.57 b |
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Miao, Q.; Li, J.-G.; Miao, S.; Hu, N.; Zhang, J.; Zhang, S.; Xie, Y.-H.; Wang, J.-B.; Wang, S.-W. The Bone-Protective Effect of Genistein in the Animal Model of Bilateral Ovariectomy: Roles of Phytoestrogens and PTH/PTHR1 Against Post-Menopausal Osteoporosis. Int. J. Mol. Sci. 2012, 13, 56-70. https://doi.org/10.3390/ijms13010056
Miao Q, Li J-G, Miao S, Hu N, Zhang J, Zhang S, Xie Y-H, Wang J-B, Wang S-W. The Bone-Protective Effect of Genistein in the Animal Model of Bilateral Ovariectomy: Roles of Phytoestrogens and PTH/PTHR1 Against Post-Menopausal Osteoporosis. International Journal of Molecular Sciences. 2012; 13(1):56-70. https://doi.org/10.3390/ijms13010056
Chicago/Turabian StyleMiao, Qing, Jing-Ge Li, Shan Miao, Nan Hu, Jin Zhang, Song Zhang, Yan-Hua Xie, Jian-Bo Wang, and Si-Wang Wang. 2012. "The Bone-Protective Effect of Genistein in the Animal Model of Bilateral Ovariectomy: Roles of Phytoestrogens and PTH/PTHR1 Against Post-Menopausal Osteoporosis" International Journal of Molecular Sciences 13, no. 1: 56-70. https://doi.org/10.3390/ijms13010056