Preparation of a Cu(II)-PVA/PA6 Composite Nanofibrous Membrane for Enzyme Immobilization
Abstract
:1. Introduction
2. Results and Discussion
2.1. Adsorption Isotherm of Cu(II) on PVA/PA6 Composite Nanofibrous Membrane
2.2. FTIR Spectra of Nanofibers
2.3. The Surface Morphologies of Nanofibrous Membrane
2.4. Kinetic Parameters of Immobilized and Free Catalases
2.5. Effect of pH and Temperature on the Enzyme Activity
2.6. Storage Stability and Reusability
3. Materials and Methods
3.1. Materials
3.2. Electrospinning
3.3. Chelation of Cu(II)
3.4. Enzyme Immobilization
3.5. Activity Assays
3.6. Determination of Kinetic Parameters
3.7. Dependence of Temperature and Value of pH
3.8. Storage Stability and Reusability
4. Conclusions
Acknowledgments
References
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Amount of bound enzyme (mg/g fibers) | Specific activity (Units/mg) | Km (mM) | Vmax (μmol/mg·min) | |
---|---|---|---|---|
Free catalase | 3400 | 26.815 | 4878 | |
Immobilized catalases | −64 | 2150 | 41.132 | 3774 |
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
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Feng, Q.; Tang, B.; Wei, Q.; Hou, D.; Bi, S.; Wei, A. Preparation of a Cu(II)-PVA/PA6 Composite Nanofibrous Membrane for Enzyme Immobilization. Int. J. Mol. Sci. 2012, 13, 12734-12746. https://doi.org/10.3390/ijms131012734
Feng Q, Tang B, Wei Q, Hou D, Bi S, Wei A. Preparation of a Cu(II)-PVA/PA6 Composite Nanofibrous Membrane for Enzyme Immobilization. International Journal of Molecular Sciences. 2012; 13(10):12734-12746. https://doi.org/10.3390/ijms131012734
Chicago/Turabian StyleFeng, Quan, Bin Tang, Qufu Wei, Dayin Hou, Songmei Bi, and Anfang Wei. 2012. "Preparation of a Cu(II)-PVA/PA6 Composite Nanofibrous Membrane for Enzyme Immobilization" International Journal of Molecular Sciences 13, no. 10: 12734-12746. https://doi.org/10.3390/ijms131012734