Lack of Association of Estrogen Receptor Alpha Gene Polymorphisms with Cardiorespiratory and Metabolic Variables in Young Women
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
3.1. Subjects and Study Design
3.1.1. Clinical and Biochemical Assessment
3.1.2. Clinical and Biochemical Assessment
3.2. Heart Rate Variability Analysis
3.3. Genotyping
3.4. Statistical Analysis
4. Conclusions
Acknowledgments
References
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Demographic and clinical data | Metabolic variables | ||
---|---|---|---|
Age, years | 26 ± 4 | Glucose, mg/dL | 71 ± 9 |
Body mass, kg | 72 ± 2 | Urea, mg/dL | 0.54 ± 0.1 |
Height, cm | 68 ± 13 | Creatinine, mg/dL | 0.6 ± 0.5 |
BMI, kg/m2 | 21 ± 3 | Total cholesterol, mg/dL | 161.2 ± 18 |
HR supine, bpm | 60 ± 5 | LDL cholesterol, mg/dL | 96.0 ± 21 |
HR sitting, bpm | 74 ± 10 | HDL cholesterol, mg/dL | 45.8 ± 10 |
SBP supine, mmHg | 110 ± 4 | Triglycerides, mg/dL | 73.0± 22 |
DBP supine, mmHg | 75 ± 3 | Progesterone, ng/mL | 1.84 ± 6 |
SBP sitting, mmHg | 115 ± 3 | Estradiol, pg/mL | 101.9 ± 56 |
DBP sitting, mmHg | 72 ± 3 | Aerobic classification AHA | Regular |
Polymorphisms | Genotypes | Alleles | |||
---|---|---|---|---|---|
c.454-397T > C (rs2234693) | TT 40.7% (144) | TC 36.2% (128) | CC 23.1% (82) | T 58.8% | C 41.2% |
c.454-351A > G (rs9340799) | AA 42.4% (150) | AG 36.4% (129) | GG 21.2% (75) | A 60.6% | G 39.4% |
Haplotypes | A–T 59.2% | G–C 35.7% | G–T 5.7% | A–C 3.9% |
Variables | c.454-397T > C genotypes | c.454-351A > G genotypes | ||||||
---|---|---|---|---|---|---|---|---|
TT (n = 144) | TC (n = 128) | CC (n = 82) | p | AA (n = 150) | AG (n = 129) | GG (n = 75) | p | |
HRV and VO2 indices | ||||||||
rMSSD, ms2 | 53.79 ± 34.39 | 53.42 ± 29.37 | 53.40 ± 41.82 | 0.43 | 53.13 ± 33.70 | 51.92 ± 31.40 | 53.16 ± 45.00 | 0.27 |
SDNN, ms2 | 55.54 ± 25.27 | 54.78 ± 23.83 | 57.34 ± 35.57 | 0.23 | 55.88 ± 26.17 | 53.36 ± 24.88 | 54.93 ± 37.39 | 0.24 |
LF, nu | 0.45 ± 0.17 | 0.43 ± 0.16 | 0.46 ± 0.16 | 0.25 | 0.45 ± 0.17 | 0.44 ± 0.16 | 0.44 ± 0.18 | 0.27 |
HF, nu | 0.54 ± 0.17 | 0.56 ± 0.16 | 0.53 ± 0.16 | 0.19 | 0.54 ± 0.17 | 0.55 ± 0.17 | 0.55 ± 0.18 | 0.71 |
LF/HF ratio | 1.08 ± 0.87 | 1.01 ± 0.94 | 1.12 ± 1.02 | 0.21 | 1.06 ± 0.86 | 1.03 ± 0.88 | 1.07 ± 1.08 | 0.33 |
VO2, mL·kg−1·min−1 | 26.28 ± 5.53 | 27.24 ± 6.23 | 26.46 ± 15.60 | 0.12 | 25.66 ± 5.57 | 26.86 ± 5.88 | 26.46 ± 5.35 | 0.43 |
Metabolic | ||||||||
Total cholesterol, mg/dL | 166 ± 32 | 165 ± 35 | 161.6 ± 39 | 0.12 | 164 ± 34 | 164 ± 35 | 164 ± 39 | 0.19 |
HDL cholesterol, mg/dL | 44 ± 13 | 45 ± 12 | 42 ± 9 | 0.14 | 44 ± 11 | 44 ± 12 | 43 ± 10 | 0.27 |
LDL cholesterol, mg/dL | 98 ± 32 | 102 ± 37 | 97 ± 40 | 0.22 | 98 ± 33 | 100 ± 35 | 100 ± 41 | 0.32 |
Triglycerides, mg/dL | 88 ± 47 | 85 ± 36 | 89 ± 59 | 0.43 | 84 ± 45 | 88 ± 41 | 89 ± 61 | 0.27 |
HRV indices | Aerobic capacity VO2 | Lipids | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
HDL cholesterol | LDL cholesterol | Total Cholesterol | Triglycerides | ||||||||
B | p | B | p | B | p | B | p | B | p | R2 | |
rMSSD | 1.40 | 0.003 | 0.24 | 0.200 | −0.10 | 0.290 | −0.18 | 0.004 | −1.79 | 0.020 | 48% |
SDNN | 1.01 | 0.001 | 0.08 | 0.590 | −0.10 | 0.240 | −0.15 | 0.002 | −0.19 | 0.010 | 30% |
LF | −1.73 | 0.010 | −2.96 | 0.040 | −0.09 | 0.960 | −0.06 | 0.13 | 0.39 | 0.840 | 34% |
HF | 19.18 | 0.004 | 2.10 | 0.280 | −0.01 | 0.700 | −0.06 | 0.03 | −2.45 | 0.150 | 42% |
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Share and Cite
Rebelo, A.C.; Verlengia, R.; Kunz, V.; Tamburus, N.; Cerda, A.; Hirata, R.; Hirata, M.; Silva, E. Lack of Association of Estrogen Receptor Alpha Gene Polymorphisms with Cardiorespiratory and Metabolic Variables in Young Women. Int. J. Mol. Sci. 2012, 13, 13691-13703. https://doi.org/10.3390/ijms131013691
Rebelo AC, Verlengia R, Kunz V, Tamburus N, Cerda A, Hirata R, Hirata M, Silva E. Lack of Association of Estrogen Receptor Alpha Gene Polymorphisms with Cardiorespiratory and Metabolic Variables in Young Women. International Journal of Molecular Sciences. 2012; 13(10):13691-13703. https://doi.org/10.3390/ijms131013691
Chicago/Turabian StyleRebelo, Ana Cristina, Rozangela Verlengia, Vandeni Kunz, Nayara Tamburus, Alvaro Cerda, Rosario Hirata, Mario Hirata, and Ester Silva. 2012. "Lack of Association of Estrogen Receptor Alpha Gene Polymorphisms with Cardiorespiratory and Metabolic Variables in Young Women" International Journal of Molecular Sciences 13, no. 10: 13691-13703. https://doi.org/10.3390/ijms131013691