Molecular Diversity Assessment Using Sequence Related Amplified Polymorphism (SRAP) Markers in Vicia faba L.
Abstract
:1. Introduction
2. Results
3. Discussion
4. Experimental Section
4.1. Plant Materials and DNA Extraction
4.2. SRAP-PCR
4.3. Data Scoring and Statistical Analysis
5. Conclusions
Acknowledgements
References
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Primer combination | Total fragments a | Average fragments b | Total no. of fragments c | PIC value |
---|---|---|---|---|
ME1/EM1 | 85 | 18 | 1,047 | 0.97 |
ME1/EM2 | 158 | 26 | 1,563 | 0.99 |
ME1/EM3 | 134 | 31 | 1,817 | 0.99 |
ME1/EM4 | 56 | 11 | 662 | 0.97 |
ME2/EM1 | 46 | 8 | 444 | 0.95 |
ME2/EM2 | 108 | 24 | 1,417 | 0.98 |
ME2/EM4 | 10 | 3 | 190 | 0.84 |
ME3/EM1 | 77 | 17 | 1,013 | 0.97 |
ME3/EM2 | 69 | 7 | 414 | 0.97 |
ME3/EM3 | 66 | 5 | 323 | 0.98 |
ME3/EM4 | 54 | 10 | 567 | 0.96 |
ME4/EM2 | 59 | 5 | 323 | 0.97 |
ME4/EM3 | 16 | 4 | 220 | 0.92 |
ME4/EM4 | 98 | 12 | 700 | 0.98 |
Total | 1,036 | - | 10,700 | - |
Average | 74 | 13 | 764 | 0.96 |
Entry No. | Entry name | Origin | Seed type |
---|---|---|---|
1 | Hassawi1 | KSA | Equine |
2 | Hassawi2 | KSA | Equine |
3 | Hassawi3 | KSA | Equine |
4 | Goff1 | KSA | Equine |
5 | T.W.(red seed) | KSA | Equine |
6 | H4 | KSA | Equine |
7 | H7 | KSA | Equine |
8 | Line 9 | KSA | Equine |
9 | Line 5 | KSA | Equine |
10 | Line 22 | KSA | Equine |
11 | Pop.6 | KSA | Equine |
12 | H3 | KSA | Equine |
13 | H5 | KSA | Equine |
14 | H8 | KSA | Equine |
15 | L. 4 | KSA | Equine |
16 | Pop. 3 | KSA | Equine |
17 | Pop. 4 | KSA | Equine |
18 | Giza 3 | KSA | Equine |
19 | Giza 4 | Egypt | Equine |
20 | Giza 40 | Egypt | Equine |
21 | Giza 402 | Egypt | Equine |
22 | Giza 429 | Egypt | Equine |
23 | Giza 461 | Egypt | Equine |
24 | Gizablanka | Egypt | Major |
25 | 1013/694/95 | Egypt | Equine |
26 | 1026/811/95 | Egypt | Equine |
27 | 985/252/95 | Egypt | Equine |
28 | 989/303/95 | Egypt | Equine |
29 | Misr 1 | Egypt | Equine |
30 | Sakha 2 | Egypt | Major |
31 | Sakha 3 | Egypt | Equine |
32 | Giza 716 | Egypt | Major |
33 | Giza 717 | Egypt | Equine |
34 | Giza 843 | Egypt | Equine |
35 | 1016/752/95 | Egypt | Equine |
36 | 987/255/95 | Egypt | Major |
37 | 989/306/95 | Egypt | Major |
38 | 989/309/95 | Egypt | Major |
39 | Sakha 1 | Egypt | Equine |
40 | Sakha 4 | Egypt | Equine |
41 | Giza 674 | Egypt | Equine |
42 | Giza 714 | Egypt | Equine |
43 | Cairo 7 | Egypt | Equine |
44 | ILB 4338 | ICARDA | Equine |
45 | ILB 4357 | ICARDA | Equine |
46 | ILB 1814 | ICARDA | Major |
47 | Ahnacya 2 | ICARDA | Equine |
48 | ILB 4347 | ICARDA | Equine |
49 | ILB 4358 | ICARDA | Major |
50 | Pakistani | Pakistan | Minor |
51 | Luz | Spain | Major |
52 | Aquadolce | Spain | Major |
53 | Kamline | Spain | Minor |
54 | Sudan | Sudan | Equine |
55 | Gazira 1 | Sudan | Major |
56 | Gazira 2 | Sudan | Minor |
57 | T.W. | Sudan | Equine |
58 | Yamani(Large seed) | Yemen | Major |
Forward primers | 5′→3′ | Reverse primers | 5′→3′ |
---|---|---|---|
ME1 | TGAGTCCAAACCGGAA | EM1 | GACTGCGTACGAATTAAT |
ME2 | TGAGTCCAAACCGGAC | EM2 | GACTGCGTACGAATTTGC |
ME3 | TGAGTCCAAACCGGAT | EM3 | GACTGCGTACGAATTGAC |
ME4 | TGAGTCCAAACCGGAC | EM4 | GACTGCGTACGAATTTGA |
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Alghamdi, S.S.; Al-Faifi, S.A.; Migdadi, H.M.; Khan, M.A.; EL-Harty, E.H.; Ammar, M.H. Molecular Diversity Assessment Using Sequence Related Amplified Polymorphism (SRAP) Markers in Vicia faba L.. Int. J. Mol. Sci. 2012, 13, 16457-16471. https://doi.org/10.3390/ijms131216457
Alghamdi SS, Al-Faifi SA, Migdadi HM, Khan MA, EL-Harty EH, Ammar MH. Molecular Diversity Assessment Using Sequence Related Amplified Polymorphism (SRAP) Markers in Vicia faba L.. International Journal of Molecular Sciences. 2012; 13(12):16457-16471. https://doi.org/10.3390/ijms131216457
Chicago/Turabian StyleAlghamdi, Salem S., Sulieman A. Al-Faifi, Hussein M. Migdadi, Muhammad Altaf Khan, Ehab H. EL-Harty, and Megahed H. Ammar. 2012. "Molecular Diversity Assessment Using Sequence Related Amplified Polymorphism (SRAP) Markers in Vicia faba L." International Journal of Molecular Sciences 13, no. 12: 16457-16471. https://doi.org/10.3390/ijms131216457