Response Surface Methodology to Optimize Enzymatic Preparation of Deapio-Platycodin D and Platycodin D from Radix Platycodi
Abstract
:1. Introduction
2. Results and Discussion
2.1. Selection of Glycolytic Enzymes
2.2. Model Fitting
2.3. Analysis of Response Surface
2.4. Optimal Conditions and Model Verification
2.5. LC/ESI-MS Analysis and Structures Elucidation
3. Experimental Section
3.1. Plant Materials and Chemicals
3.2. Sample Preparation
3.3. Enzymatic Preparation of dPD and PD from Crude Platycosides
3.4. HPLC Analysis of Platycosides
3.5. LC/ESI-MS Analysis
3.6. Experimental Design
3.7. Data Analysis
4. Conclusions
Acknowledgments
References
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Coded variables levels | Y dPD and PD (mg/L) | ||||
---|---|---|---|---|---|
Run | X1, Reaction temperature (°C) | X2, Enzyme load (%) | X3, Reaction time (h) | Actual | Predicted |
1 | −1 (35) | −1 (5.0) | 0 (14) | 7.28 | 7.45 |
2 | 1 (45) | −1 (5.0) | 0 (14) | 12.00 | 11.46 |
3 | −1 (35) | 1 (20) | 0 (14) | 8.90 | 9.44 |
4 | 1 (45) | 1 (20) | 0 (14) | 13.22 | 13.05 |
5 | −1 (35) | 0 (12.5) | −1 (4) | 8.63 | 8.18 |
6 | 1 (45) | 0 (12.5) | −1 (4) | 11.73 | 11.98 |
7 | −1 (35) | 0 (12.5) | 1 (24) | 10.92 | 10.67 |
8 | 1 (45) | 0 (12.5) | 1 (24) | 14.02 | 14.48 |
9 | 0 (40) | −1 (5.0) | −1 (4) | 9.03 | 9.32 |
10 | 0 (40) | 1 (20) | −1 (4) | 10.25 | 10.16 |
11 | 0 (40) | −1 (5.0) | 1 (24) | 10.79 | 10.87 |
12 | 0 (40) | 1 (20) | 1 (24) | 13.89 | 13.60 |
13 | 0 (40) | 0 (12.5) | 0 (14) | 13.75 | 13.67 |
14 | 0 (40) | 0 (12.5) | 0 (14) | 13.08 | 13.67 |
15 | 0 (40) | 0 (12.5) | 0 (14) | 13.89 | 13.67 |
16 | 0 (40) | 0 (12.5) | 0 (14) | 13.89 | 13.67 |
17 | 0 (40) | 0 (12.5) | 0 (14) | 13.75 | 13.67 |
Source | SS | DF | MS | F-value | Prob > F | |
---|---|---|---|---|---|---|
Model | 78.13 | 9 | 8.68 | 33.43 | <0.0001 | significant |
Residual | 1.82 | 7 | 0.26 | |||
Lack of fit | 1.36 | 3 | 0.45 | 3.96 | 0.1086 | insignificant |
Pure error | 0.46 | 4 | 0.11 |
Variables | DF | SS | MS | F-values | p-value |
---|---|---|---|---|---|
X1 | 1 | 29.02 | 29.02 | 111.78 | <0.0001 |
X2 | 1 | 6.39 | 6.39 | 24.59 | 0.0016 |
X3 | 1 | 12.45 | 12.45 | 47.94 | 0.0002 |
X1X2 | 1 | 0.041 | 0.041 | 0.16 | 0.7032 |
X1X3 | 1 | 0.000 | 0.000 | 0.000 | 1.0000 |
X2X3 | 1 | 0.89 | 0.89 | 3.43 | 0.1064 |
X12 | 1 | 9.39 | 9.39 | 36.17 | 0.0005 |
X22 | 1 | 14.11 | 14.11 | 54.34 | 0.0002 |
X32 | 1 | 3.06 | 3.06 | 11.80 | 0.0109 |
Reaction temperature (°C) | Enzyme load (%) | Reaction time (h) | Yield of dPD and PD (mg/mL) | |
---|---|---|---|---|
Optimum conditions | 43.13 | 15.00 | 22.23 | 14.93 (predicted) |
Modified conditions | 43 | 15 | 22 | 14.81 (actual) |
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
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Li, W.; Zhao, L.-C.; Wang, Z.; Zheng, Y.-N.; Liang, J.; Wang, H. Response Surface Methodology to Optimize Enzymatic Preparation of Deapio-Platycodin D and Platycodin D from Radix Platycodi. Int. J. Mol. Sci. 2012, 13, 4089-4100. https://doi.org/10.3390/ijms13044089
Li W, Zhao L-C, Wang Z, Zheng Y-N, Liang J, Wang H. Response Surface Methodology to Optimize Enzymatic Preparation of Deapio-Platycodin D and Platycodin D from Radix Platycodi. International Journal of Molecular Sciences. 2012; 13(4):4089-4100. https://doi.org/10.3390/ijms13044089
Chicago/Turabian StyleLi, Wei, Li-Chun Zhao, Zi Wang, Yi-Nan Zheng, Jian Liang, and Hui Wang. 2012. "Response Surface Methodology to Optimize Enzymatic Preparation of Deapio-Platycodin D and Platycodin D from Radix Platycodi" International Journal of Molecular Sciences 13, no. 4: 4089-4100. https://doi.org/10.3390/ijms13044089