An Efficient Intergeneric Conjugation of DNA from Escherichia coli to Mycelia of the Lincomycin-Producer Streptomyces lincolnensis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Plasmids
2.2. Media and Culture Conditions
2.3. Conjugation Method
2.4. PCR Cloning of Lincomycin Resistance Genes and Construction of Over-Expression Vectors
2.5. Lincomycin Extraction and HPLC Analysis
3. Results and Discussion
3.1. Optimization of Liquid Media for Recipient Mycelia Growth of S. lincolnensis
3.2. Optimization of Recipient Mycelia Growth Conditions
3.3. Optimization of Solid Media for Exconjugants Regeneration
3.4. Ratio of Donor Cell Numbers to Recipient Mycelia
3.5. Over-Expression of lmrABC in S. lincolnensis
4. Conclusions
Acknowledgment
References
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Du, L.; Liu, R.-H.; Ying, L.; Zhao, G.-R. An Efficient Intergeneric Conjugation of DNA from Escherichia coli to Mycelia of the Lincomycin-Producer Streptomyces lincolnensis. Int. J. Mol. Sci. 2012, 13, 4797-4806. https://doi.org/10.3390/ijms13044797
Du L, Liu R-H, Ying L, Zhao G-R. An Efficient Intergeneric Conjugation of DNA from Escherichia coli to Mycelia of the Lincomycin-Producer Streptomyces lincolnensis. International Journal of Molecular Sciences. 2012; 13(4):4797-4806. https://doi.org/10.3390/ijms13044797
Chicago/Turabian StyleDu, Lei, Rui-Hua Liu, Li Ying, and Guang-Rong Zhao. 2012. "An Efficient Intergeneric Conjugation of DNA from Escherichia coli to Mycelia of the Lincomycin-Producer Streptomyces lincolnensis" International Journal of Molecular Sciences 13, no. 4: 4797-4806. https://doi.org/10.3390/ijms13044797