Isolation and Identification of Fourteen Microsatellite Markers in Clivia miniata and Clivia nobilis (Amaryllidaceae)
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
3.1. Isolation of Microsatellite Markers
3.2. Detection of Polymorphism and Data Analysis
4. Conclusions
Acknowledgments
References
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Clivia miniata (N = 51) | Hybrid (N = 2) | Clivia nobilis (N = 8) | |||||||
---|---|---|---|---|---|---|---|---|---|
Locus | NA | HE | HO | NA | HE | HO | NA | HE | HO |
CM9 | 3 | 0.17 | 0.18 | 1 | 0.00 | 0.00 | 1 | 0.00 | 0.00 |
CM12 | 4 | 0.22 | 0.18 | 3 | 0.83 | 0.50 | 3 | 0.49 | 0.38 |
CM54 | 1 | 0.00 | 0.00 | 1 | 0.00 | 0.00 | 2 | 0.22 | 0.00 |
CM65 | 2 | 0.09 | 0.10 | 1 | 0.00 | 0.00 | 1 | 0.00 | 0.00 |
CM103 | 4 | 0.48 | 0.53 | 2 | 0.50 | 0.50 | 1 | 0.00 | 0.00 |
CM137 | 2 | 0.04 | 0.04 | 1 | 0.00 | 0.00 | 1 | 0.00 | 0.00 |
CM289 | 3 | 0.44 | 0.65 | 2 | 0.50 | 0.50 | 4 | 0.42 | 0.38 |
CM357 | 5 | 0.56 | 0.76 | 3 | 0.83 | 0.83 | 2 | 0.20 | 0.00 |
CN68 | 4 | 0.23 | 0.16 | 1 | 0.00 | 0.00 | 3 | 0.30 | 0.00 |
CN106 | 2 | 0.50 | 1.00 | 2 | 0.67 | 1.00 | 2 | 0.47 | 0.88 |
Locus | Primer sequences (5′–3′) | Repeat | Size (bp) | Ta | NA | GenBank |
---|---|---|---|---|---|---|
CM9 | F: TTACCTCCTCCAACTCACAG R: ATGAAAACGGCCCGAATTAC | (TG)9 | 251 | 48 | 3 | JQ782265 |
CM12 | F: CAACCTCAAGCTCAGTCTCA R: AGGTGCAGGATGATAATGGT | (AC)6T(AC)2 | 230 | 47 | 4 | JQ782266 |
CM54 | F: GAGCAACTCAGGAAGGGATG R: CCGCTAAGGACTTTACGCAC | (GT)16 | 194 | 46 | 2 | JQ782267 |
CM65 | F: AGGGTGAGAGTGTAGGTGTG R: CTTAGGCATGTATAGTGGCC | (GT)4T(TG)4 | 190 | 48 | 2 | JQ782268 |
CM103 | F: ACCCCTTACACAGACTACCA R: GATGGGTAGAAGGTGGTTGT | (AC)15G(CA)21 | 313 | 47 | 4 | JQ782271 |
CM137 | F: TTCTTGCCGACAATCCCGTA R: TGATGGTCGCAAAATTCACG | (AC)6 | 288 | 47 | 2 | JQ782273 |
CM289 | F: GGATATTATGTTGCCAAGCC R: AAAGTTGAATCGCGGTCCCA | (TG)11 | 207 | 58 | 5 | JQ782275 |
CM357 | F: GCGAATGATAATCGTGAACC R: TCCTCCTTTTCTCACACTTC | (GT)16 | 160 | 46 | 6 | JQ782276 |
CN68 | F: TTCTGACTTACACCTACACA R: ATTATGCACTTGGATCTAGG | (AC)17 | 167 | 47 | 5 | JQ782269 |
CN106 | F: AACAGCAGGAAATTAGGGAG R: TCACAAGCATACACACACAC | (TG)16 | 195 | 48 | 2 | JQ782272 |
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Gao, W.; Zhao, H.; Liu, Y.; Li, M.-R.; Nurmamat, E.; Li, L.-F.; Ren, Y.-Y.; Xiao, H.-X. Isolation and Identification of Fourteen Microsatellite Markers in Clivia miniata and Clivia nobilis (Amaryllidaceae). Int. J. Mol. Sci. 2012, 13, 9609-9614. https://doi.org/10.3390/ijms13089609
Gao W, Zhao H, Liu Y, Li M-R, Nurmamat E, Li L-F, Ren Y-Y, Xiao H-X. Isolation and Identification of Fourteen Microsatellite Markers in Clivia miniata and Clivia nobilis (Amaryllidaceae). International Journal of Molecular Sciences. 2012; 13(8):9609-9614. https://doi.org/10.3390/ijms13089609
Chicago/Turabian StyleGao, Wei, He Zhao, Ying Liu, Ming-Rui Li, Eliyas Nurmamat, Lin-Feng Li, Yue-Ying Ren, and Hong-Xing Xiao. 2012. "Isolation and Identification of Fourteen Microsatellite Markers in Clivia miniata and Clivia nobilis (Amaryllidaceae)" International Journal of Molecular Sciences 13, no. 8: 9609-9614. https://doi.org/10.3390/ijms13089609